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NEURON SPECIFIC ENOLASE (ENO2) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS211833

MBS211833 is an antibody targeting ENO2. Also known as MOUSE ANTI HUMAN NEURON SPECIFIC ENOLASE. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
NEURON SPECIFIC ENOLASE (ENO2), Monoclonal Antibody
Full Product Name
MOUSE ANTI HUMAN NEURON SPECIFIC ENOLASE
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
5G10
Host
Mouse
Species Reactivity
Human
Form/Format
Purified (Purified IgG - liquid)
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Specificity
NEURON SPECIFIC ENOLASE
This item is specific for human neuron specific enolase (NSE), a glycolytic enzyme that is expressed in neuronal tissue and is released as a result of damage to neural tissue. An increase in serum levels of NSE has been found in patients with tumours such as neuroblastoma.
Preparation and Storage
Store at +4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of dispatch.
Molecular Weight
47,269 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (7023614..7032859). Location: 12p13
OMIM
131360
3D Structure
ModBase 3D Structure for P09104
Immunogen
Native, from brain
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
Buffer Solution
Phosphate buffered saline
Applications Tested/Suitable for anti-ENO2 antibody
Immunohistology - Paraffin (IHC), ELISA (EIA)
Application Notes for anti-ENO2 antibody
Immunohistology - Paraffin: Min Dilution: 1/100
Max Dilution: 1/400
Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Histology Positive Control Tissue: Brain cortex

Testing Data #1 of anti-ENO2 antibody

Human Cortical Neurons stained with Mouse anti Human Neuron Specific Enolase (MBS211833)
Mouse Anti-ENO2 Monoclonal Antibody – Cat# MBS211833

Other Notes

Small volumes of anti-ENO2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ENO2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
gamma-enolase
NCBI Official Synonym Full Names
enolase 2 (gamma, neuronal)
NCBI Official Symbol
ENO2
NCBI Official Synonym Symbols
NSE
UniProt Protein Name
Gamma-enolase
UniProt Synonym Protein Names
2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase
UniProt Gene Name
ENO2
UniProt Synonym Gene Names
NSE
UniProt Entry Name
ENOG_HUMAN
NCBI Protein Information
gamma-enolase; neural enolase; neuron-specific enolase; neurone-specific enolase; neuron specific gamma enolase; 2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ENO2 antibody

 Reference Product  PubMed Publications
 HK1 antibody  >1 publications with ENO2 and HK1
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