Published customer image:Morphology and immunostaining of IL-4 DC and IL-10 DC. Rat BMDC were isolated from cell clusters on day 6 of culture (A). Cells prepared on cytospin slides stained positive for monoclonal antibodies Ox62 (rat DC marker) (B), Ox6 (MHC class II) (C), and CD68 (D). Shown are representative IL-4 DC results, which are similar to those for IL-10 DC. Magnification: x200 (A, C, D) and x400 (B).From: Tiurbe G, Matuschek A, K¤mmerer U, Schneider M, Thiede A, Ulrichs K, Otto C. Inhibitory effects of rat bone marrow-derived dendritic cells on na¯ve and alloantigen-specific CD4+ T cells: a comparison between dendritic cells generated with GM-CSF plus IL-4 and dendritic cells generated with GM-CSF plus IL-10. BMC Res Notes. 2009 Jan 23;2:12.

Published customer image: The expression of OX62+DCs and OX62+CD4+SIRP+DCs of each groups at various development stages (Mean +/- SD). (a) The expression level of OX62+DCs of each group at different stages. (b) The expression level of OX62+CD4+SIRP+ DCs of each group at different stages. (c) Single-cell suspensions of the total PP-DCs in rats were identified by OX62. The difference of OX62+DC among groups at different development stages was not significant. (F = 3.0, 0.587, 3.267, and 1.471, resp.; P >.05). Significant growth occurred in LR group for the number of OX62+CD4+SIRP+DCs at age week 3. Levels of OX62+CD4+SIRP+DC subsets at every age were highly significant in LR group and BR group compared with AR group. Furthermore, the positive cell numbers were higher in LR group than in BR group. The positive cell numbers kept stable in LR group at various ages while the positive cells number increased with age in the other two groups. Significant numbers of OX62+CD4+SIRP+DCs were found in LR and BR group at various ages (Figures 1(b) and 1(c)) compared with AR group (A: BR at W3, B: AR at W3, C: LR at W3, D: BR at W5, E: AR at W5, F: LR at W5, G: BR at W7, H: AR at W7, I: LR at W7, J: BR at W11, K: AR at W11, L: LR at W11). *P <.05.From: Yi-Jun Zhou, Juan Gao, Hua-Mei Yang, Xiang-Liang Yuan, Tong-Xin Chen, and Zhen-Juan He, œThe Role of the Lactadherin in Promoting Intestinal DCs Development In Vivo and Vitro, Clinical and Developmental Immunology, vol. 2010, Article ID 357541, 9 pages, 2010.

Rat Splenocytes stained with Mouse anti Rat OX-62:RPE (MBS213538)

Published customer image: Analysis of innate immune cells into a corneal graft. Ox-62+ DC and CD163+ macrophages were stained at the time points of corneal allograft rejection and calculated within the graft. Additionally CD161+ cells were counted within rejected corneal allografts to finally prove the efficacy of the depletion protocol in the peripheral tissue. Representative histological staining is shown for Ox-62 (A), CD163 (C), and CD161 (E) in NK deficient and control animals. B: No statistical difference was observed for infiltrating Ox-62+ DC. D: CD163+ macrophages infiltrated to a statistically significantly stronger extent in 3.2.3-treated animals when compared to control treated animals (*p