• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CD16 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS212047

MBS212047 is an antibody targeting CD16. Also known as MOUSE ANTI HUMAN CD16. Host: Mouse. More details

$550 / 0.2 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD16, Monoclonal Antibody
Full Product Name
MOUSE ANTI HUMAN CD16
Product Gene Name
Product Synonym Names
CD16A, CD16B, FCG3, FCGR3, IGFR3, FcRIII
Clonality
Monoclonal
Isotype
IgG1
Clone Number
LNK16
Host
Mouse
Form/Format
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Specificity
Mouse anti Human CD16 antibody, clone LNK16 recognizes CD16, a 50-65kDa cell surface molecule which exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes.

CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Mouse anti Human CD16 antibody, clone LNK16 has been reported to block the binding of IgG to the CD16 molecule (Tamm and Schmidt 1996).
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C. Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
OMIM
610665
Target Species
Human
Preparation
Purified IgG prepared by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
Normal human peripheral blood granulocytes.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
Applications Tested/Suitable for anti-CD16 antibody
Flow cytometry (FC), Immunoprecipitation (IP)
Application Notes for anti-CD16 antibody
FC - Suggested Dilution: 1/10 - 1/20
Use 10 ul of the suggested working dilution to label 106 cells or 100ul human whole blood.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Testing Data of anti-CD16 antibody

Staining of human peripheral blood granulocytes with Mouse anti Human CD16: Biotin (MBS210312)
Mouse Anti-CD16 Monoclonal Antibody – Cat# MBS212047

Testing Data of anti-CD16 antibody

Staining of human peripheral blood lymphocytes with Mouse anti Human CD16: Low Endotoxin (MBS211192)
Mouse Anti-CD16 Monoclonal Antibody, Testing Data – Cat# MBS212047

Testing Data of anti-CD16 antibody

Staining of human peripheral blood granulocytes with Mouse anti Human CD16
Mouse Anti-CD16 Monoclonal Antibody, Testing Data – Cat# MBS212047

Testing Data of anti-CD16 antibody

Staining of human peripheral blood granulocytes with Mouse anti Human CD16: FITC (MBS210627)
Mouse Anti-CD16 Monoclonal Antibody, Testing Data – Cat# MBS212047

Testing Data of anti-CD16 antibody

Staining of human peripheral blood granulocytes with Mouse anti Human CD16:Alexa Fluor 647 (MBS212047)
Mouse Anti-CD16 Monoclonal Antibody, Testing Data – Cat# MBS212047

Other Notes

Small volumes of anti-CD16 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD16. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
low affinity immunoglobulin gamma Fc region receptor III-B isoform 2
NCBI Official Synonym Full Names
Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
NCBI Official Symbol
FCGR3B
NCBI Official Synonym Symbols
CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor III-B
UniProt Synonym Protein Names
Fc-gamma RIII-beta; Fc-gamma RIII; Fc-gamma RIIIb; FcRIII; FcRIIIb; FcR-10; IgG Fc receptor III-1; CD_antigen: CD16b
Protein Family
UniProt Gene Name
FCGR3B
UniProt Synonym Gene Names
CD16B; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIb; FcRIII; FcRIIIb
UniProt Entry Name
FCG3B_HUMAN
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor III-B; Fc-gamma receptor IIIb (CD 16); fc-gamma RIII-beta; fc-gamma RIIIb; igG Fc receptor III-1

NCBI Summary for CD16

The protein encoded by this gene is a low affinity receptor for the Fc region of gamma immunoglobulins (IgG). The encoded protein acts as a monomer and can bind either monomeric or aggregated IgG. This gene may function to capture immune complexes in the peripheral circulation. Several transcript variants encoding different isoforms have been found for this gene. A highly-similar gene encoding a related protein is also found on chromosome 1. [provided by RefSeq, Aug 2012]
Show more

UniProt Comments for CD16

Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD16 antibody

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000