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HISTIDINE Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS212126

Mouse anti Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged… More details

$965 / 1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HISTIDINE TAG, Monoclonal Antibody
Full Product Name
MOUSE ANTI HISTIDINE TAG
Clonality
Monoclonal
Isotype
IgG1
Clone Number
AD1.1.10
Host
Mouse
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
PAX6 transcription factor linked to histidine tag
Fusion Partners||Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Buffer Solution||Phosphate buffered saline
Target Species
Synthetic Peptide
Applications Tested/Suitable for anti-HISTIDINE TAG antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-HISTIDINE TAG antibody
Western Blotting: Minimum Dilution: 1/500; Maximum Dilution: 1/1000

Testing Data of anti-HISTIDINE TAG antibody

Western blot of HIS tagged protein probed with Mouse anti Histidine tag : Biotin (MBS210332) and visualised with Streptavidin : HRP (MBS238416)
Mouse Anti-HISTIDINE Monoclonal Antibody – Cat# MBS212126

Testing Data of anti-HISTIDINE TAG antibody

Western blot of HIS tagged protein probed with Mouse anti Histidine tag:Dylight800 (MBS212126D800GA). Visualised using a LI-COR Odyssey infrared imaging system
Mouse Anti-HISTIDINE Monoclonal Antibody, Testing Data – Cat# MBS212126

Testing Data of anti-HISTIDINE TAG antibody

Human anti Human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualised with Mouse anti-HIS tag: FITC antibody (MBS210678)
Mouse Anti-HISTIDINE Monoclonal Antibody, Testing Data – Cat# MBS212126

Testing Data of anti-HISTIDINE TAG antibody

Western blot of HIS-tagged protein probed with Mouse anti Histidine tag (MBS216898) visualised with Rabbit anti Mouse IgG : HRP (MBS235192)
Mouse Anti-HISTIDINE Monoclonal Antibody, Testing Data – Cat# MBS212126

Testing Data of anti-HISTIDINE TAG antibody

His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag
Mouse Anti-HISTIDINE Monoclonal Antibody, Testing Data – Cat# MBS212126

Other Notes

Small volumes of anti-HISTIDINE TAG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HISTIDINE TAG purchase

MBS212126 is an antibody targeting HISTIDINE TAG. Also known as MOUSE ANTI HISTIDINE TAG. Host: Mouse.
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