• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CD19 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS212150

MBS212150 is an antibody targeting CD19. Also known as RAT ANTI MOUSE CD19. Host: Rat. More details

$420 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD19, Monoclonal Antibody
Full Product Name
RAT ANTI MOUSE CD19
Product Gene Name
anti-CD19 antibody
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
6D5
Host
Rat
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
60,149 Da
Perservative Stabilisers
0.09% Sodium Azide
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Human K562 cell line transfected with murine CD19
Fusion Partners||Spleen cells from immunised rats were fused with cells of the P3X63.Ag8.653 myeloma cell line.
Buffer Solution||Phosphate buffered saline
Target Species
Mouse
Applications Tested/Suitable for anti-CD19 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes for anti-CD19 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/50

Testing Data of anti-CD19 antibody

Staining of mouse spleen cells with Rat anti Mouse CD19:RPE (MBS213605)
Rat Anti-CD19 Monoclonal Antibody – Cat# MBS212150

Testing Data of anti-CD19 antibody

Staining of mouse spleen cells with Rat anti Mouse CD19:RPE-Alexa Fluor 647 (MBS212149P647)
Rat Anti-CD19 Monoclonal Antibody, Testing Data – Cat# MBS212150

Testing Data of anti-CD19 antibody

Staining of mouse spleen cells with Rat anti Mouse CD19:FITC (MBS210692)
Rat Anti-CD19 Monoclonal Antibody, Testing Data – Cat# MBS212150

Testing Data of anti-CD19 antibody

Staining of mouse spleen cells with Rat anti Mouse CD19:APC (MBS210012)
Rat Anti-CD19 Monoclonal Antibody, Testing Data – Cat# MBS212150

Testing Data of anti-CD19 antibody

Immunoperoxidase staining of a murine lymph node with Rat anti Mouse CD19 antibody, clone 6D5 (MBS212149) forllowerd by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195). High power
Rat Anti-CD19 Monoclonal Antibody, Testing Data – Cat# MBS212150

Testing Data of anti-CD19 antibody

Staining of mouse spleen cells with Rat anti Mouse CD19 (MBS212149)
Rat Anti-CD19 Monoclonal Antibody, Testing Data – Cat# MBS212150

Testing Data of anti-CD19 antibody

Immunoperoxidase staining of a murine lymph node with Rat anti Mouse CD19 antibody, clone 6D5 (MBS212149) forllowerd by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195). Low power
Rat Anti-CD19 Monoclonal Antibody, Testing Data – Cat# MBS212150

Testing Data of anti-CD19 antibody

Immunofluorescence staining of a mouse lymph node cryosection with Rat anti MouseCD19 antibody, clone 6D5 (MBS212149), green in A and Rat anti Mlouse CD8 antibody, clone YTS105.18 , red in B. Merged image in C with nuclei counterstained blue using DAPI. High power
Rat Anti-CD19 Monoclonal Antibody, Testing Data – Cat# MBS212150

Testing Data of anti-CD19 antibody

Staining of mouse spleen cells with Rat anti Mouse CD19:RPE-Alexa Fluor 750 (MBS212149P750)
Rat Anti-CD19 Monoclonal Antibody, Testing Data – Cat# MBS212150

Testing Data of anti-CD19 antibody

Immunofluorescence staining of a mouse lymph node cryosection with Rat anti MouseCD19 antibody, clone 6D5 (MBS212149), green in A and Rat anti Mlouse CD8 antibody, clone YTS105.18 , red in B. Merged image in C with nuclei counterstained blue using DAPI. Low power
Rat Anti-CD19 Monoclonal Antibody, Testing Data – Cat# MBS212150

Other Notes

Small volumes of anti-CD19 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD19. It may not necessarily be applicable to this product.

NCBI GI #
146149173
NCBI GeneID
12478
NCBI Accession #
NP_033974.2
UniProt Secondary Accession #
Q542B2
UniProt Related Accession #
P25918
GenBank Nucleotide #
NM_009844.2
NCBI Official Full Name
B-lymphocyte antigen CD19
NCBI Official Synonym Full Names
CD19 antigen
NCBI Official Symbol
Cd19
NCBI Official Synonym Symbols
AW495831
UniProt Protein Name
B-lymphocyte antigen CD19
UniProt Synonym Protein Names
Differentiation antigen CD19; CD_antigen: CD19
Protein Family
B-lymphocyte antigen
UniProt Gene Name
Cd19
UniProt Entry Name
CD19_MOUSE
NCBI Protein Information
B-lymphocyte antigen CD19; differentiation antigen CD19

UniProt Comments for CD19

CD19: a cell surface molecule which assembles with the antigen receptor of B lymphocytes. A critical signal transduction molecule that regulates B lymphocyte development, activation, and differentiation. Ligation increases intracellular calcium levels and decreases the threshold for antigen receptor signaling. Germinal center formation is significantly reduced in CD19-deficient mice.

Protein type: Cell surface; Membrane protein, integral

Cellular Component: protein complex; membrane; integral to membrane; external side of plasma membrane

Biological Process: B cell receptor signaling pathway; positive regulation of release of sequestered calcium ion into cytosol
Show more

Research Articles on CD19

1. This inhibitory function of FcgammaRIIB in impairing the spatial-temporal colocalization of BCR and CD19 microclusters in the B cell immunological synapse may help explain the hyper-reactive features of systemic lupus erythematosus

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD19 antibody

 Reference Product  PubMed Publications
 Cd5 antibody  >1056 publications with CD19 and Cd5
 Cd79a antibody  >150 publications with CD19 and Cd79a
 Cd79b antibody  >107 publications with CD19 and Cd79b
 Cd81 antibody  >96 publications with CD19 and Cd81
 Cr2 antibody  >75 publications with CD19 and Cr2
 Lyn antibody  >57 publications with CD19 and Lyn
 Btk antibody  >45 publications with CD19 and Btk
 Vav1 antibody  >13 publications with CD19 and Vav1
 Pik3r1 antibody  >2 publications with CD19 and Pik3r1

Pathways associated with anti-CD19 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway antibodies  Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram

Diseases associated with anti-CD19 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >446 publications with CD19 and Cardiovascular Diseases
 Inflammation Antibodies  >362 publications with CD19 and Inflammation
 Respiratory Tract Diseases Antibodies  >329 publications with CD19 and Respiratory Tract Diseases
 Necrosis Antibodies  >326 publications with CD19 and Necrosis
 Death Antibodies  >265 publications with CD19 and Death
 Lung Diseases Antibodies  >209 publications with CD19 and Lung Diseases
 Liver Diseases Antibodies  >164 publications with CD19 and Liver Diseases
 Cell Transformation, Neoplastic Antibodies  >138 publications with CD19 and Cell Transformation, Neoplastic
 Respiratory Tract Infections Antibodies  >112 publications with CD19 and Respiratory Tract Infections
 Common Variable Immunodeficiency Antibodies  >91 publications with CD19 and Common Variable Immunodeficiency

Organs/Tissues associated with anti-CD19 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >6247 publications with CD19 and Blood
 Bone Marrow Antibodies  >1741 publications with CD19 and Bone Marrow
 Spleen Antibodies  >578 publications with CD19 and Spleen
 Lymph Node Antibodies  >479 publications with CD19 and Lymph Node
 Liver Antibodies  >311 publications with CD19 and Liver
 Thymus Antibodies  >175 publications with CD19 and Thymus
 Intestine Antibodies  >106 publications with CD19 and Intestine
 Embryonic Tissue Antibodies  >95 publications with CD19 and Embryonic Tissue
 Joint Antibodies  >84 publications with CD19 and Joint
 Adipose Tissue Antibodies  >20 publications with CD19 and Adipose Tissue
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000