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CD14 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS212217

MBS212217 is an antibody targeting CD14. Also known as MOUSE ANTI HUMAN CD14. Reactive in Bovine, Cat, Cynomolgus monkey, Dog, Goat, Llama, Mink, Pig, Rabbit, Sheep. Host: Mouse. More details

$580 / 0.2 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD14, Monoclonal Antibody
Full Product Name
MOUSE ANTI HUMAN CD14
Product Gene Name
anti-CD14 antibody
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
TÜK4
Host
Mouse
Species Reactivity
Bovine, Cat, Cynomolgus monkey, Dog, Goat, Llama, Mink, Pig, Rabbit, Sheep
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
40,076 Da
OMIM
158120
Perservative Stabilisers
0.09% Sodium Azide
Preparation||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Target Species
Human
Applications Tested/Suitable for anti-CD14 antibody
ELISA (EIA), Flow cytometry (FC/FACS), Western Blot (WB)
Application Notes for anti-CD14 antibody
Flow Cytometry: Use 5ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/200

Testing Data of anti-CD14 antibody

Staining of human peripheral blood monocytes with Mouse anti Human CD14:Pacific Blue (MBS212217PB)
Mouse Anti-CD14 Monoclonal Antibody – Cat# MBS212217

Testing Data of anti-CD14 antibody

Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE-Alexa Fluor 647 (MBS212217P647)
Mouse Anti-CD14 Monoclonal Antibody, Testing Data – Cat# MBS212217

Testing Data of anti-CD14 antibody

Published customer image: Immunophenotyping gating strategy and morphological analysis for MDSC identification in peripheral blood of dogs. PBMCs from healthy dogs and dogs with cancer were stained for the myeloid marker CD11b, monocytic marker CD14 and MHC II. (A) Representative flow cytometric analysis of forward and side scatter and gated CD11b+CD14-MHCII- cells from dogs with advanced or metastatic tumors compared to dogs with early stage non-metastatic tumors and healthy control dogs. Plots are representative of dog with advanced metastatic hemangiosarcoma (top), early stage bladder transitional cell carcinoma (middle) and a healthy dog. (B) FACS sorted CD11b+CD14-MHCII- cells were stained with diff-quick for cell morphology evaluation. A representative example of polymorphonuclear granulocyte morphology of CD11b+CD14-MHCII- cells is shown at 63x magnification.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.
Mouse Anti-CD14 Monoclonal Antibody, Testing Data – Cat# MBS212217

Testing Data of anti-CD14 antibody

Published customer image: CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.
Mouse Anti-CD14 Monoclonal Antibody, Testing Data – Cat# MBS212217

Testing Data of anti-CD14 antibody

Staining of human peripheral blood monocytes with Mouse anti Human CD14:Biotin (MBS219364)
Mouse Anti-CD14 Monoclonal Antibody, Testing Data – Cat# MBS212217

Testing Data of anti-CD14 antibody

Staining of human peripheral blood monocytes with Mouse anti Human CD14 (MBS212218)
Mouse Anti-CD14 Monoclonal Antibody, Testing Data – Cat# MBS212217

Testing Data of anti-CD14 antibody

Published customer image: CD11b+CD14+MHCII- cells demonstrate ability to suppressive T cell proliferation. (A) CD11b+CD14+MHCII- cells were sorted from peripheral blood sample of an osteosarcoma dog (B) and co-cultured with healthy dog PBMCs in the presence of mitogen for 72 hs. Non-stimulated PBMCs were used as negative control and PBMCs co-cultured with healthy PMNs were used to control for the effect of adding cells to the assay. Proliferative responses were measured by 3H-thymidine incorporation. CPM, counts per minute. The experiment was performed in triplicate. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.
Mouse Anti-CD14 Monoclonal Antibody, Testing Data – Cat# MBS212217

Testing Data of anti-CD14 antibody

Staining of human peripheral blood monocytes with Mouse anti Human CD14 (MBS211203)
Mouse Anti-CD14 Monoclonal Antibody, Testing Data – Cat# MBS212217

Testing Data of anti-CD14 antibody

Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE-Alexa Fluor 750 (MBS212217P750)
Mouse Anti-CD14 Monoclonal Antibody, Testing Data – Cat# MBS212217

Testing Data of anti-CD14 antibody

Staining of human peripheral blood monocytes probed with Mouse anti Human CD14:FITC (MBS210731)
Mouse Anti-CD14 Monoclonal Antibody, Testing Data – Cat# MBS212217

Other Notes

Small volumes of anti-CD14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD14. It may not necessarily be applicable to this product.

NCBI GI #
4557417
NCBI GeneID
929
NCBI Accession #
NP_000582.1
UniProt Secondary Accession #
Q53XT5; Q96FR6; Q96L99; Q9UNS3
UniProt Related Accession #
P08571
GenBank Nucleotide #
NM_000591.3
NCBI Official Full Name
monocyte differentiation antigen CD14
NCBI Official Synonym Full Names
CD14 molecule
NCBI Official Symbol
CD14
UniProt Protein Name
Monocyte differentiation antigen CD14
UniProt Synonym Protein Names
Myeloid cell-specific leucine-rich glycoprotein; CD_antigen: CD14Cleaved into the following 2 chains:Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form
Protein Family
Monocyte differentiation antigen
UniProt Gene Name
CD14
UniProt Entry Name
CD14_HUMAN
NCBI Protein Information
monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein

NCBI Summary for CD14

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]
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UniProt Comments for CD14

CD14: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; cell surface; plasma membrane; extracellular region; endosome membrane; lipid raft

Molecular Function: peptidoglycan receptor activity; protein binding; opsonin receptor activity; lipopolysaccharide binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; apoptosis; MyD88-independent toll-like receptor signaling pathway; positive regulation of endocytosis; toll-like receptor 3 signaling pathway; positive regulation of tumor necrosis factor production; phagocytosis; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to magnesium ion; response to ethanol; cell surface receptor linked signal transduction; response to heat; toll-like receptor signaling pathway; innate immune response; inflammatory response; response to electrical stimulus; toll-like receptor 4 signaling pathway; positive regulation of cytokine secretion
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Research Articles on CD14

1. CD14 -260C/T polymorphism may increase the risk of gastric cancer in H. pylori-infected individuals. (Meta-analysis)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD14 antibody

 Reference Product  PubMed Publications
 TLR4 antibody  >1380 publications with CD14 and TLR4
 TLR2 antibody  >727 publications with CD14 and TLR2
 LBP antibody  >430 publications with CD14 and LBP
 ITGB2 antibody  >317 publications with CD14 and ITGB2
 LY96 antibody  >147 publications with CD14 and LY96
 TLR1 antibody  >101 publications with CD14 and TLR1
 TLR6 antibody  >62 publications with CD14 and TLR6
 LY86 antibody  >9 publications with CD14 and LY86

Pathways associated with anti-CD14 antibody

 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway antibodies  Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 IKK Complex Recruitment Mediated By RIP1 Pathway antibodies  IKK Complex Recruitment Mediated By RIP1 Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram

Diseases associated with anti-CD14 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2319 publications with CD14 and Inflammation
 Necrosis Antibodies  >2310 publications with CD14 and Necrosis
 Cardiovascular Diseases Antibodies  >647 publications with CD14 and Cardiovascular Diseases
 Lung Diseases Antibodies  >531 publications with CD14 and Lung Diseases
 Liver Diseases Antibodies  >360 publications with CD14 and Liver Diseases
 Nervous System Diseases Antibodies  >359 publications with CD14 and Nervous System Diseases
 Disease Models, Animal Antibodies  >330 publications with CD14 and Disease Models, Animal
 Atherosclerosis Antibodies  >311 publications with CD14 and Atherosclerosis
 Heart Diseases Antibodies  >280 publications with CD14 and Heart Diseases
 Asthma Antibodies  >259 publications with CD14 and Asthma

Organs/Tissues associated with anti-CD14 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >7524 publications with CD14 and Blood
 Bone Antibodies  >1329 publications with CD14 and Bone
 Vascular Antibodies  >827 publications with CD14 and Vascular
 Liver Antibodies  >622 publications with CD14 and Liver
 Lung Antibodies  >597 publications with CD14 and Lung
 Kidney Antibodies  >334 publications with CD14 and Kidney
 Brain Antibodies  >307 publications with CD14 and Brain
 Heart Antibodies  >298 publications with CD14 and Heart
 Muscle Antibodies  >281 publications with CD14 and Muscle
 Intestine Antibodies  >238 publications with CD14 and Intestine
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