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CD47 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS212449

Clone OX-101 recognizes the rat homologue of human CD47, which has been identified as the ligand for the rat signal regulatory protein (SIRP). Rat CD47 is expressed primarily by lymphocytes and macrophages but has also been detected at high levels on brain… More details

$670 / 0.25 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD47, Monoclonal Antibody
Full Product Name
MOUSE ANTI RAT CD47
Product Gene Name
anti-CD47 antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
OX-101
Host
Mouse
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Specificity
Clone OX-101 recognizes the rat homologue of human CD47, which has been identified as the ligand for the rat signal regulatory protein (SIRP). Rat CD47 is expressed primarily by lymphocytes and macrophages but has also been detected at high levels on brain endothelial cells.Clone OX-101 has been reported to block the interaction of CD47 and SIRP.
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
34,776 Da
3D Structure
ModBase 3D Structure for P97829
Perservative Stabilisers
0.09% Sodium Azide
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Rat thymocytes
Buffer Solution||Phosphate buffered saline
Target Species
Rat
Applications Tested/Suitable for anti-CD47 antibody
Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-CD47 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Testing Data of anti-CD47 antibody

Staining of Rat Spleen cells with Mouse anti Rat CD47:FITC (MBS235335)
Mouse Anti-CD47 Monoclonal Antibody – Cat# MBS212449

Testing Data of anti-CD47 antibody

Staining of Rat Spleen cells with Mouse anti Rat CD47:Alexa Fluor 647 (MBS212449A647)
Mouse Anti-CD47 Monoclonal Antibody, Testing Data – Cat# MBS212449

Testing Data of anti-CD47 antibody

Staining of Rat Spleen cells with Mouse anti Rat CD47:RPE (MBS213705)
Mouse Anti-CD47 Monoclonal Antibody, Testing Data – Cat# MBS212449

Other Notes

Small volumes of anti-CD47 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD47 purchase

MBS212449 is an antibody targeting CD47. Also known as MOUSE ANTI RAT CD47. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD47. It may not necessarily be applicable to this product.

NCBI GI #
9506469
NCBI GeneID
29364
NCBI Accession #
NP_062068.1
UniProt Primary Accession #
P97829
UniProt Secondary Accession #
O35294
UniProt Related Accession #
P97829
GenBank Nucleotide #
NM_019195.2
NCBI Official Full Name
leukocyte surface antigen CD47
NCBI Official Synonym Full Names
Cd47 molecule
NCBI Official Symbol
Cd47
NCBI Official Synonym Symbols
Itgp
UniProt Protein Name
Leukocyte surface antigen CD47
UniProt Synonym Protein Names
Integrin-associated protein; IAP; CD_antigen: CD47
Protein Family
Leukocyte surface antigen
UniProt Gene Name
Cd47
UniProt Synonym Gene Names
IAP
UniProt Entry Name
CD47_RAT
NCBI Protein Information
leukocyte surface antigen CD47; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); IAP; integrin-associated protein

NCBI Summary for CD47

protein that may be involved in regulating renal cell growth; overexpression is associated with Fe-NTA-induced renal damage and tumor progression and metastasis [RGD, Feb 2006]

UniProt Comments for CD47

CD47: Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Membrane protein, multi-pass

Cellular Component: integral to plasma membrane; integral to membrane; plasma membrane

Biological Process: integrin-mediated signaling pathway; cell migration; response to bacterium; positive regulation of phagocytosis; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; positive regulation of T cell activation; opsonization; cell adhesion; positive regulation of inflammatory response
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Research Articles on CD47

1. The CD47-binding peptide of thrombospondin-1 induces defenestration of liver sinusoidal endothelial cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD47 antibody

 Products by Pathway  Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Integrin Cell Surface Interactions Pathway antibodies  Integrin Cell Surface Interactions Pathway Diagram
 Signal Regulatory Protein (SIRP) Family Interactions Pathway antibodies  Signal Regulatory Protein (SIRP) Family Interactions Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram

Diseases associated with anti-CD47 antibody

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >87 publications with CD47 and Immune System Diseases
 Inflammation Antibodies  >83 publications with CD47 and Inflammation
 Disease Models, Animal Antibodies  >48 publications with CD47 and Disease Models, Animal
 Anemia Antibodies  >43 publications with CD47 and Anemia
 Necrosis Antibodies  >43 publications with CD47 and Necrosis
 Nervous System Diseases Antibodies  >34 publications with CD47 and Nervous System Diseases
 Brain Diseases Antibodies  >30 publications with CD47 and Brain Diseases
 Neoplasms, Experimental Antibodies  >23 publications with CD47 and Neoplasms, Experimental
 Respiratory Tract Diseases Antibodies  >14 publications with CD47 and Respiratory Tract Diseases
 Diabetes Mellitus Antibodies  >11 publications with CD47 and Diabetes Mellitus

Organs/Tissues associated with anti-CD47 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >58 publications with CD47 and Brain
 Lung Antibodies  >28 publications with CD47 and Lung
 Liver Antibodies  >28 publications with CD47 and Liver
 Spleen Antibodies  >27 publications with CD47 and Spleen
 Kidney Antibodies  >17 publications with CD47 and Kidney
 Heart Antibodies  >13 publications with CD47 and Heart
 Connective Tissue Antibodies  >12 publications with CD47 and Connective Tissue
 Thymus Antibodies  >11 publications with CD47 and Thymus
 Embryonic Tissue Antibodies  >11 publications with CD47 and Embryonic Tissue
 Ovary Antibodies  >10 publications with CD47 and Ovary
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