• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CD177 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS212458

Mouse anti Human CD177 antibody, clone MEM-166 recognizes human CD177 (neutrophil glycoprotein NB1). The neutrophil NB1 antigen is expressed by 97% of the caucasian population. Antibodies against NB1 can be involved in neonatal alloimmune neutropenia (NAN). More details

$460 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD177, Monoclonal Antibody
Full Product Name
MOUSE ANTI HUMAN CD177
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1
Clone Number
MEM-166
Host
Mouse
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
15,683 Da
OMIM
162860
3D Structure
ModBase 3D Structure for Q8N6Q3
Perservative Stabilisers
0.09% Sodium Azide
Preparation||Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Target Species
Human
Applications Tested/Suitable for anti-CD177 antibody
Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)*
Application Notes for anti-CD177 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Western Blotting: Maximum Dilution: Non-reducing conditions; Application Note: MEM-166 recognizes CD177 under non-reducing conditions

Testing Data of anti-CD177 antibody

Staining of human peripheral blood lymphocytes with Mouse anti Human CD56:Alexa Fluor 647 (MBS212459A647)
Mouse Anti-CD177 Monoclonal Antibody – Cat# MBS212458

Testing Data of anti-CD177 antibody

Staining of human peripheral blood granulocytes with Mouse anti Human CD177:FITC (MBS210825)
Mouse Anti-CD177 Monoclonal Antibody, Testing Data – Cat# MBS212458

Testing Data of anti-CD177 antibody

Staining of human peripheral blood granulocytes with Mouse anti Human CD177:RPE (MBS213707)
Mouse Anti-CD177 Monoclonal Antibody, Testing Data – Cat# MBS212458

Other Notes

Small volumes of anti-CD177 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD177 purchase

MBS212458 is an antibody targeting CD177. Also known as MOUSE ANTI HUMAN CD177. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD177. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
CD177 antigen
NCBI Official Synonym Full Names
CD177 molecule
NCBI Official Symbol
CD177
NCBI Official Synonym Symbols
NB1; PRV1; HNA2A; PRV-1; HNA-2a; NB1 GP
UniProt Protein Name
CD177 antigen
UniProt Synonym Protein Names
Human neutrophil alloantigen 2a; HNA-2a; NB1 glycoprotein; NB1 GP; Polycythemia rubra vera protein 1; PRV-1; CD_antigen: CD177
Protein Family
UniProt Gene Name
CD177
UniProt Synonym Gene Names
NB1; PRV1; HNA-2a; NB1 GP; PRV-1
UniProt Entry Name
CD177_HUMAN
NCBI Protein Information
CD177 antigen; NB1 glycoprotein; cell surface receptor; human neutrophil alloantigen 2a; polycythemia rubra vera protein 1

NCBI Summary for CD177

This gene encodes a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation. The protein can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in this gene are associated with myeloproliferative diseases. Over-expression of this gene has been found in patients with polycythemia rubra vera. Autoantibodies against the protein may result in pulmonary transfusion reactions, and it may be involved in Wegener's granulomatosis. A related pseudogene, which is adjacent to this gene on chromosome 19, has been identified. [provided by RefSeq, Apr 2014]
Show more

UniProt Comments for CD177

CD177: Induced by CSF3 in resting granulocytes. Induced in patients with polycythemia vera (PV) and with essential thrombocythemia (ET). 3 isoforms of the human protein are produced by alternative splicing

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: plasma membrane

Biological Process: blood coagulation; leukocyte migration
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD177 antibody

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000