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CD152 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS212485

Mouse anti Rat CD152 antibody, clone WKH203 recognizes rat CD152, also known as cytoxic-T-lymphocyte antigen-4 (CTLA-4), which is similar in structure to CD28 and also binds ligands CD80 and CD86. CD152 is expressed by activated T-lymphocytes and studies also… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CD152, Monoclonal Antibody
Full Product Name
MOUSE ANTI RAT CD152
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1
Clone Number
WKH203
Host
Mouse
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Perservative Stabilisers
0.09% Sodium Azide
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Purified rCTLA-4hIg fusion protein.
Fusion Partners||Spleen cells from immunised Balb/c were fused with cells of the X63-Ag8-653 myeloma cell line.
Buffer Solution||Phosphate buffered saline
Target Species
Rat
Applications Tested/Suitable for anti-CD152 antibody
ELISA (EIA), Flow cytometry (FC/FACS)*, Western Blot (WB)
Application Notes for anti-CD152 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/20; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.

Testing Data of anti-CD152 antibody

Staining of rat CD152 transfected cell line using Mouse anti Rat CD152 following permeabilisation with Leucoperm
Mouse Anti-CD152 Monoclonal Antibody – Cat# MBS212485

Other Notes

Small volumes of anti-CD152 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD152 purchase

MBS212485 is an antibody targeting CD152. Also known as MOUSE ANTI RAT CD152. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD152. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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