Mouse Anti-GZMB Monoclonal Antibody – Cat# MBS212505

GRANZYME B (GZMB) Antibody – Mouse Monoclonal

Name: GZMB Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS212505
Price: 420 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS212505

Mouse anti Human Granzyme B antibody, clone GB7 recognizes the serine protease Granzyme B, which is expressed within the granules of cytolytic lymphocytes (CTLs) Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target… More details

$420 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GRANZYME B (GZMB), Monoclonal Antibody

Full Product Name

MOUSE ANTI HUMAN GRANZYME B

Product Gene Name

anti-GZMB antibody

Clonality

Monoclonal

Isotype

IgG2a

Clone Number

GB7

Host

Mouse

Form/Format

Purified
Purified IgG - liquid

Concentration

IgG concentration 1.0 mg/ml (lot specific)

Preparation and Storage

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Molecular Weight

27,716 Da

OMIM

123910

3D Structure

ModBase 3D Structure for P10144

Perservative Stabilisers

0.01% Thiomersal
Preparation||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Immunogen

Recombinant Granzyme B
Fusion Partners||Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Buffer Solution||TRIS buffered saline

Target Species

Human

Applications Tested/Suitable for anti-GZMB antibody

Immunohistology Paraffin*, Western Blot (WB)

Application Notes for anti-GZMB antibody

Immunohistology - Paraffin: Minimum Dilution: 1/50; Maximum Dilution: 1/200; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Other Notes

Small volumes of anti-GZMB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GZMB purchase

MBS212505 is an antibody targeting GZMB. Also known as MOUSE ANTI HUMAN GRANZYME B. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GZMB. It may not necessarily be applicable to this product.

NCBI GI #

221625528

NCBI GeneID

3002

NCBI Accession #

NP_004122.2

UniProt Primary Accession #

P10144

UniProt Secondary Accession #

Q8N1D2; Q9UCC1

UniProt Related Accession #

P10144

GenBank Nucleotide #

NM_004131.4

NCBI Official Full Name

granzyme B

NCBI Official Synonym Full Names

granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)

NCBI Official Symbol

GZMB

NCBI Official Synonym Symbols

HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1

UniProt Protein Name

Granzyme B

UniProt Synonym Protein Names

C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E

Protein Family

Granzyme

UniProt Gene Name

GZMB

UniProt Synonym Gene Names

CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP

UniProt Entry Name

GRAB_HUMAN

NCBI Protein Information

granzyme B; C11; CTLA-1; T-cell serine protease 1-3E; cathepsin G-like 1; cytotoxic T-lymphocyte proteinase 2; cytotoxic serine protease B; fragmentin 2; fragmentin-2; human lymphocyte protein

NCBI Summary for GZMB

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq, Jul 2008]

UniProt Comments for GZMB

GZMB: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. By staphylococcal enterotoxin A (SEA) in peripheral blood leukocytes. Inactivated by the serine protease inhibitor diisopropylfluorophosphate. Belongs to the peptidase S1 family. Granzyme subfamily.

Protein type: Protease; Apoptosis; EC 3.4.21.79

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: intracellular membrane-bound organelle; membrane; cytoplasm; immunological synapse; nucleus; cytosol

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: Notch signaling pathway; induction of apoptosis by granzyme; natural killer cell mediated cytotoxicity; apoptosis; cytolysis; immune response; protein processing; proteolysis

Research Articles on GZMB

1. Serum concentrations of granzyme B in patients with ovarian cancer were substantially increased in comparison to concentrations in patients with ovarian cystadenomas or ovarian teratomas.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GZMB antibody

Reference Product	PubMed Publications
PRF1 antibody	>1284 publications with GZMB and PRF1
CASP3 antibody	>146 publications with GZMB and CASP3
BID antibody	>62 publications with GZMB and BID
SERPINB9 antibody	>60 publications with GZMB and SERPINB9
IL2RA antibody	>51 publications with GZMB and IL2RA
CASP7 antibody	>20 publications with GZMB and CASP7
IL2RB antibody	>14 publications with GZMB and IL2RB
IGF2R antibody	>13 publications with GZMB and IGF2R
IL2 antibody	>6 publications with GZMB and IL2

Pathways associated with anti-GZMB antibody

Products by Pathway	Pathway Diagram
Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway antibodies	Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Caspase Cascade In Apoptosis Pathway antibodies	Caspase Cascade In Apoptosis Pathway Diagram
Downstream Signaling In Naive CD8+ T Cells Pathway antibodies	Downstream Signaling In Naive CD8+ T Cells Pathway Diagram

Diseases associated with anti-GZMB antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>318 publications with GZMB and Necrosis
Inflammation Antibodies	>210 publications with GZMB and Inflammation
Disease Models, Animal Antibodies	>123 publications with GZMB and Disease Models, Animal
Neoplasms, Experimental Antibodies	>99 publications with GZMB and Neoplasms, Experimental
Liver Diseases Antibodies	>80 publications with GZMB and Liver Diseases
Hepatitis Antibodies	>55 publications with GZMB and Hepatitis
Brain Diseases Antibodies	>37 publications with GZMB and Brain Diseases
Breast Neoplasms Antibodies	>35 publications with GZMB and Breast Neoplasms
Kidney Diseases Antibodies	>31 publications with GZMB and Kidney Diseases
Atherosclerosis Antibodies	>22 publications with GZMB and Atherosclerosis

Organs/Tissues associated with anti-GZMB antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>2148 publications with GZMB and Blood
Lung Antibodies	>163 publications with GZMB and Lung
Kidney Antibodies	>148 publications with GZMB and Kidney
Liver Antibodies	>141 publications with GZMB and Liver
Spleen Antibodies	>139 publications with GZMB and Spleen
Bone Marrow Antibodies	>96 publications with GZMB and Bone Marrow
Heart Antibodies	>95 publications with GZMB and Heart
Intestine Antibodies	>66 publications with GZMB and Intestine
Pancreas Antibodies	>34 publications with GZMB and Pancreas
Uterus Antibodies	>21 publications with GZMB and Uterus