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CD66b Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS212531

Mouse anti Human CD66b antibody, clone 80H3 reacts with the CGM6 gene product of the carcinoembryonic gene family. This is a 95-100kDa GPI anchored molecule. CD66b is expressed strongly by mature granulocytes and metamyelocytes, and weakly by bone marrow… More details

$610 / 0.2 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD66b, Monoclonal Antibody
Full Product Name
MOUSE ANTI HUMAN CD66b
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1
Clone Number
80H3
Host
Mouse
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
38,154 Da
OMIM
114890
3D Structure
ModBase 3D Structure for P31997
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Preparation||Purified IgG prepared by affinity chromatography on Protein A from ascites
Immunogen
CML leucocytes
Histology Positive Control Tissue||Bone Marrow
Fusion Partners||Spleen cells from immunised BALB/c mice were fused with cells of the mouse MOPC 315-43 myeloma cell line.
Buffer Solution||Phosphate buffered saline.
Target Species
Human
Applications Tested/Suitable for anti-CD66b antibody
Immunohistology Frozen*, Flow cytometry (FC/FACS), Functional Assays (FN)*, Western Blot (WB)*
Application Notes for anti-CD66b antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 5 x 105 cells or 100ul whole blood.
Immunohistology - Frozen: Minimum Dilution: 1/25; Maximum Dilution: 1/100; Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Functional Assays: Application Note: This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes
Western Blotting: Application Note: See Feuk-Lagerstedt et al.

Other Notes

Small volumes of anti-CD66b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD66b purchase

MBS212531 is an antibody targeting CD66b. Also known as MOUSE ANTI HUMAN CD66b. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD66b. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
carcinoembryonic antigen-related cell adhesion molecule 8
NCBI Official Synonym Full Names
carcinoembryonic antigen-related cell adhesion molecule 8
NCBI Official Symbol
CEACAM8
NCBI Official Synonym Symbols
CD67; CGM6; CD66b; NCA-95
UniProt Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 8
UniProt Synonym Protein Names
CD67 antigen; Carcinoembryonic antigen CGM6; Non-specific cross-reacting antigen NCA-95; CD_antigen: CD66b
UniProt Gene Name
CEACAM8
UniProt Synonym Gene Names
CGM6
UniProt Entry Name
CEAM8_HUMAN
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 8; CD67 antigen; carcinoembryonic antigen CGM6; carcinoembryonic antigen gene family member 6; non-specific cross-reacting antigen NCA-95

UniProt Comments for CD66b

CEACAM8: Belongs to the immunoglobulin superfamily. CEA family.

Protein type: Membrane protein, GPI anchor; Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Biological Process: immune response; blood coagulation; leukocyte migration
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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