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CORONAVIRUS Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS212561

Viruses are finicky particles, much like feline attributes. Coronavirus, a member of the influenza virus family, is a major health threat to cats so much research is devoted to FIPV. To accelerated detection assays by flow cytometry, to increase understanding… More details

$695 / 0.25 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CORONAVIRUS, Monoclonal Antibody
Full Product Name
MOUSE ANTI FELINE CORONAVIRUS
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
FIPV3-70
Host
Mouse
Species Reactivity
Feline
Form/Format
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Specificity
Mouse anti Feline Coronavirus antibody, clone FIPV3-70 reacts with feline infectious peritonitis virus (FIPV) type 1 and 2 and is known to be specific for the nucleocapsid. It is also known to react with canine coronavirus (CCV), pig coronavirus transmissible gastroenteritis virus (TGEV) and ferret coronavirus. Some specific activity has been detected against bovine coronavirus (BCV). Mouse anti Feline Coronavirus antibody, clone FIPV3-70 exhibits negative reactivity against Feline Leukemia virus, Feline Immunodeficiency virus, Feline Calcivirus, Feline Herpes virus, Canine Adenovirus (type 2), Canine Distemper virus, Canine Parvovirus and Canine Parainfluenza virus.
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Target Species
Viral
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilizers
<0.1% Sodium Azide (NaN3)
Immunogen
Coronavirus cocktail.
Applications Tested/Suitable for anti-CORONAVIRUS antibody
Flow Cytometry (FC), Immunohistochemistry - Frozen (IHC-Fr), Immunohistochemistry - Paraffin (IHC-P)*, ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes for anti-CORONAVIRUS antibody
WB: Under reducing conditions, MBS212561 detects a specific band of 50-56 kDa that represents the nucleocapsid.
MBS212561 detects specific bands at 56 and 46 kDa in reducing gels with CCV as antigen.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with appropriate negative/positive controls.
(1)This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.

Other Notes

Small volumes of anti-CORONAVIRUS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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