Atrial Natriuretic Peptide-Converting Enzyme (ANPCE) ELISA Kit for Rat

Name: Rat ANPCE ELISA Kit; Atrial Natriuretic Peptide-Converting Enzyme; MyBioSource
Brand: MyBioSource
SKU: MBS021270
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS021270

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated ANPCE concentrations in Rat serum, plasma and other body fluids. Using Purified Rat ANPCE antibody to coat Microelisa Stripplate wells… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Atrial Natriuretic Peptide-Converting Enzyme (ANPCE), ELISA Kit

Full Product Name

Rat Atrial Natriuretic Peptide-Converting Enzyme ELISA Kit

Product Gene Name

ANPCE elisa kit

Species Reactivity

Rat

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenate, Feces and Urine

Detection Range

0.25ng/ml-8ng/ml.

Sensitivity

0.1ng/ml.

Specificity

No significant cross-reactivity or interference between Rat ANPCE and analogues was observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

116,486 Da

Chromosome Location

Chromosome: 4; NC_000004.11 (47596018..47840059, complement). Location: 4p13-p12

OMIM

605236

3D Structure

ModBase 3D Structure for Q9Y5Q5

Intended Uses

This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated ANPCE concentrations in Rat serum, plasma and other body fluids. Using Purified Rat ANPCE antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ANPCE and ANPCE antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ANPCE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Other Notes

Small volumes of ANPCE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ANPCE purchase

MBS021270 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Atrial Natriuretic Peptide-Converting Enzyme (ANPCE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ANPCE. The ELISA analytical biochemical technique of the MBS021270 kit is based on ANPCE antibody-ANPCE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANPCE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANPCE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ANPCE. It may not necessarily be applicable to this product.

NCBI GI #

29244926

NCBI GeneID

10699

NCBI Accession #

NP_006578.2

UniProt Primary Accession #

Q9Y5Q5

UniProt Secondary Accession #

Q2TBD2; Q4W5E5; Q4W5G6; Q9UHY2; B0ZBE3

UniProt Related Accession #

Q9Y5Q5

GenBank Nucleotide #

NM_006587.3

NCBI Official Full Name

atrial natriuretic peptide-converting enzyme isoform 1

NCBI Official Synonym Full Names

corin, serine peptidase

NCBI Official Symbol

CORIN

NCBI Official Synonym Symbols

CRN; ATC2; Lrp4; PEE5; TMPRSS10

UniProt Protein Name

Atrial natriuretic peptide-converting enzyme

UniProt Synonym Protein Names

Corin; Heart-specific serine proteinase ATC2; Pro-ANP-converting enzyme; Transmembrane protease serine 10

Protein Family

Atrial natriuretic peptide-converting enzyme

UniProt Gene Name

CORIN

UniProt Synonym Gene Names

CRN; TMPRSS10

UniProt Entry Name

CORIN_HUMAN

NCBI Protein Information

atrial natriuretic peptide-converting enzyme; pro-ANP-convertase; pro-ANP-converting enzyme; transmembrane protease serine 10; heart-specific serine proteinase ATC2

NCBI Summary for ANPCE

This gene encodes a member of the type II transmembrane serine protease class of the trypsin superfamily. Members of this family are composed of multiple structurally distinct domains. The encoded protein converts pro-atrial natriuretic peptide to biologically active atrial natriuretic peptide, a cardiac hormone that regulates blood volume and pressure. This protein may also function as a pro-brain-type natriuretic peptide convertase. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

UniProt Comments for ANPCE

CORIN: Serine-type endopeptidase involved in atrial natriuretic peptide hormone (NPPA) processing. Converts through proteolytic cleavage the non-functional propeptide NPPA into the active hormone, thereby regulating blood pressure in heart and promoting natriuresis, diuresis and vasodilation. Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Also acts as a regulator of sodium reabsorption in kidney. May also process pro-NPPB the B-type natriuretic peptide. Defects in CORIN are the cause of pre-eclampsia/eclampsia 5 (PEE5); also known as gestational proteinuric hypertension. Preeclampsia is a pregnancy-associated disease with maternal symptoms but placental origin. Unlike most other human disorders, it impacts 2 individuals, the mother and her child, both of whom can be severely affected. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.-; Protease; Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 4p12

Cellular Component: cell surface; integral to plasma membrane; plasma membrane; integral to membrane; extracellular region

Molecular Function: serine-type endopeptidase activity

Biological Process: regulation of blood pressure; peptide hormone processing; female pregnancy; proteolysis; regulation of systemic arterial blood pressure by atrial natriuretic peptide

Disease: Preeclampsia/eclampsia 5

Research Articles on ANPCE

1. human CORIN gene mutations causing impaired corin activity may be an underlying mechanism in hypertension

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ANPCE elisa kit

Reference Product	PubMed Publications
NPPA elisa kit	>1 publications with ANPCE and NPPA

Pathways associated with ANPCE elisa kit

Products by Pathway	Pathway Diagram
Myometrial Relaxation And Contraction Pathways antibodies	Myometrial Relaxation And Contraction Pathways Diagram

Diseases associated with ANPCE elisa kit

Disease Name	Pubmed Publications
Heart Diseases Antibodies	>58 publications with ANPCE and Heart Diseases
Hypertrophy Antibodies	>22 publications with ANPCE and Hypertrophy
Cardiomegaly Antibodies	>21 publications with ANPCE and Cardiomegaly
Neoplasms Antibodies	>19 publications with ANPCE and Neoplasms
Brain Diseases Antibodies	>13 publications with ANPCE and Brain Diseases
Nervous System Diseases Antibodies	>11 publications with ANPCE and Nervous System Diseases
Carcinoma Antibodies	>9 publications with ANPCE and Carcinoma
Fibrosis Antibodies	>8 publications with ANPCE and Fibrosis
Kidney Diseases Antibodies	>7 publications with ANPCE and Kidney Diseases
Congenital Abnormalities Antibodies	>5 publications with ANPCE and Congenital Abnormalities

Organs/Tissues associated with ANPCE elisa kit

Organ/Tissue Name	Pubmed Publications
Heart Antibodies	>73 publications with ANPCE and Heart
Muscle Antibodies	>40 publications with ANPCE and Muscle
Eye Antibodies	>17 publications with ANPCE and Eye
Bone Antibodies	>15 publications with ANPCE and Bone
Connective Tissue Antibodies	>12 publications with ANPCE and Connective Tissue
Skin Antibodies	>7 publications with ANPCE and Skin
Embryonic Tissue Antibodies	>3 publications with ANPCE and Embryonic Tissue
Uterus Antibodies	>3 publications with ANPCE and Uterus
Intestine Antibodies	>1 publications with ANPCE and Intestine
Testis Antibodies	>1 publications with ANPCE and Testis