IgY Antibody – Mouse Monoclonal

Monoclonal

IgG1

16C7

Mouse

Goose
Does not react with Chicken
N.B. Antibody reactivity and working conditions may vary between species.

Purified IgG - liquid

IgG concentration 0.5mg/ml (lot specific)

IgY Heavy Chain

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

0.09% Sodium Azide (NaN3)

Purified Pekin duck yolk IgY.

Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

Phosphate buffered saline

Duck

MBS212832 recognizes both the IgY and IgY(-Fc) Pekin duck heavy chain molecules. The duck IgY molecule, consisting of two heavy (H) and two light (L) chains, has been categorized as the 7.8S full-length IgY form and the 5.7S truncated IgY(-Fc) form. Studies have shown that the IgY(-Fc) molecule possesses the H chain Cu1 and Cu2 constant domains, but is lacking the Cu3 and Cu4 constant region terminal domains, all four of which are present in the full-length IgY molecule. Thus the structure of the IgY(-Fc) molecule resembles that of a F(ab’)2 fragment of IgY.
MBS212832 also detects IgY heavy chain in Mallard ducks, goose and swan.
MBS212832 detects bands of approximately 118kDa and 178-200kDa in Pekin duck cell lysates under non-reducing conditions in Western blotting.