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CD169 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS212987

Mouse anti rat CD169 antibody, clone ED3 recognises the rat CD169 cell surface antigen, a 185kD molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The… More details

$605 / 0.25 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD169, Monoclonal Antibody
Full Product Name
MOUSE ANTI RAT CD169
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
ED3
Host
Mouse
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 0.5 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Perservative Stabilisers
0.09% Sodium Azide
Preparation||Purified IgG prepared by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline
Target Species
Rat
Applications Tested/Suitable for anti-CD169 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes for anti-CD169 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology - Frozen: Minimum Dilution: 1/50; Maximum Dilution: 1/250
Flow Cytometry: Maximum Dilution: 1/500

Testing Data of anti-CD169 antibody

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. Low power
Mouse Anti-CD169 Monoclonal Antibody – Cat# MBS212987

Testing Data of anti-CD169 antibody

Published customer image: Immunostaining of rat spleen. Serial-cut frozen sections were stained with mAbs towards A, rat MCL B, rat CD169 C, rat MHC class II D, human MHC class I (negative control) and visualized with peroxidase-conjugated secondary antibody and DAB substrate. RP: red pulp. PALS: periarteriolar lymphoid sheath. FOLL: follicle. MZ: marginal zone.From: Lobato-Pascual A, Saether PC, Dahle MK, Gaustad P, Dissen E, et al. (2013) Rat Macrophage C-Type Lectin Is an Activating Receptor Expressed by Phagocytic Cells. PLoS ONE 8(2): e57406.
Mouse Anti-CD169 Monoclonal Antibody, Testing Data – Cat# MBS212987

Testing Data of anti-CD169 antibody

Published customer image: Systemically administered liposomes home primarily to splenic marginal zone and red pulp macrophages. (a,b) Healthy rats were injected with 5 mg/kg DiI-labeled PCLs and PSLs. Splenic cryosections were stained with CD169 (a, marginal metallophilic and marginal zone macrophages) and CD68 (b, red pulp macrophages). One representative experiment is shown (20x magnification).From: Bogie JF, Jorissen W, Mailleux J, Nijland PG, Zelcer N, Vanmierlo T, Van Horssen J, Stinissen P, Hellings N, Hendriks JJ. Myelin alters the inflammatory phenotype of macrophages by activating PPARs. Acta Neuropathol Commun. 2013 Aug 2;1(1):43.
Mouse Anti-CD169 Monoclonal Antibody, Testing Data – Cat# MBS212987

Testing Data of anti-CD169 antibody

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a (MBS235411) as a detection reagent. Medium power
Mouse Anti-CD169 Monoclonal Antibody, Testing Data – Cat# MBS212987

Testing Data of anti-CD169 antibody

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. High power
Mouse Anti-CD169 Monoclonal Antibody, Testing Data – Cat# MBS212987

Testing Data of anti-CD169 antibody

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. Medium power
Mouse Anti-CD169 Monoclonal Antibody, Testing Data – Cat# MBS212987

Testing Data of anti-CD169 antibody

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a (MBS235411) as a detection reagent. Low power
Mouse Anti-CD169 Monoclonal Antibody, Testing Data – Cat# MBS212987

Testing Data of anti-CD169 antibody

Frozen rat spleen stained with Mouse anti Rat CD169 followed by Goat anti Mouse IgG:HRP (Rat Adosorbed, MBS235196)
Mouse Anti-CD169 Monoclonal Antibody, Testing Data – Cat# MBS212987

Testing Data of anti-CD169 antibody

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a (MBS235411) as a detection reagent. High power
Mouse Anti-CD169 Monoclonal Antibody, Testing Data – Cat# MBS212987

Other Notes

Small volumes of anti-CD169 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD169 purchase

MBS212987 is an antibody targeting CD169. Also known as MOUSE ANTI RAT CD169. Host: Mouse.
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