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CLASS I RT1A (MHC) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS213069

Mouse anti Rat MHC Class I RT1A antibody, clone OX-18 recognizes a monomorphic determinant of rat MHC Class I (RT1A), expressed by all rat strains. However, quantitative measurements suggest that not all of the class I molecules are recognized. Mouse anti Rat… More details

$440 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MHC CLASS I RT1A, Monoclonal Antibody
Full Product Name
MOUSE ANTI RAT MHC CLASS I RT1A
Clonality
Monoclonal
Isotype
IgG1
Clone Number
OX-18
Host
Mouse
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Perservative Stabilisers
0.09% Sodium Azide
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Rat spleen glycoproteins
Fusion Partners||Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
Buffer Solution||Phosphate buffered saline
Target Species
Rat
Applications Tested/Suitable for anti-MHC CLASS I RT1A antibody
Immunohistology Frozen*, ELISA (EIA), Flow cytometry (FC/FACS)*, Immunoprecipitation (IP)
Application Notes for anti-MHC CLASS I RT1A antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology: Acetone fixation recommended - the antigen is sensitive to fixation with paraformaldehyde.
Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100

Testing Data of anti-MHC CLASS I RT1A antibody

Staining of rat spleen cells with Mouse anti Rat MHC Class I (RT1A) Monomorphic: FITC (MBS216456)
Mouse Anti-MHC Monoclonal Antibody – Cat# MBS213069

Other Notes

Small volumes of anti-MHC CLASS I RT1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MHC CLASS I RT1A purchase

MBS213069 is an antibody targeting MHC CLASS I RT1A. Also known as MOUSE ANTI RAT MHC CLASS I RT1A. Host: Mouse.
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