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Cytokeratin 14 (KRT14) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS213190

Mouse anti Human Cytokeratin 14 antibody, clone LL002 recognizes cytokeratin 14, a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express… More details

$605 / 0.2 mg +1 FREE 8GB USB
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CYTOKERATIN 14 (KRT14), Monoclonal Antibody
Full Product Name
MOUSE ANTI HUMAN CYTOKERATIN 14
Product Gene Name
anti-KRT14 antibody
Clonality
Monoclonal
Isotype
IgG3
Clone Number
LL002
Host
Mouse
Species Reactivity
Dog, Elephant, Lion, Pig
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
51,561 Da
OMIM
125595
3D Structure
ModBase 3D Structure for P02533
Perservative Stabilisers
0.09% Sodium Azide
Preparation||Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Histology Positive Control Tissue
Skin
Buffer Solution||Phosphate buffered saline
Target Species
Human
Applications Tested/Suitable for anti-KRT14 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS)*, Immunofluorescence (IF), Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-KRT14 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Maximum Dilution: 1/100; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.
Immunohistology - Paraffin: Maximum Dilution: 1/200; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Testing Data of anti-KRT14 antibody

Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human cytokeratin 14 followed by HRP polymer detection and DAB substrate development (high power) following heat mediated antigen retrieval using citrate buffer at pH6.2
Mouse Anti-KRT14 Monoclonal Antibody – Cat# MBS213190

Testing Data of anti-KRT14 antibody

Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human cytokeratin 14 followed by HRP polymer detection and DAB substrate development (low power) following heat mediated antigen retrieval using citrate buffer at pH6.2
Mouse Anti-KRT14 Monoclonal Antibody, Testing Data – Cat# MBS213190

Testing Data of anti-KRT14 antibody

Immunoperoxidase staining of formalin fixed, paraffin embedded human tonsil with Mouse anti Human cytokeratin 14 folllowing heat mediated antigen retrieval using citrate buffer, pH6.0
Mouse Anti-KRT14 Monoclonal Antibody, Testing Data – Cat# MBS213190

Other Notes

Small volumes of anti-KRT14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KRT14 purchase

MBS213190 is an antibody targeting KRT14. Also known as MOUSE ANTI HUMAN CYTOKERATIN 14. Reactive in Dog, Elephant, Lion, Pig. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT14. It may not necessarily be applicable to this product.

NCBI GI #
15431310
NCBI GeneID
3861
NCBI Accession #
NP_000517.2
UniProt Primary Accession #
P02533
UniProt Secondary Accession #
Q14715; Q53XY3; Q9BUE3; Q9UBN2; Q9UBN3; Q9UCY4
UniProt Related Accession #
P02533
GenBank Nucleotide #
NM_000526.4
NCBI Official Full Name
keratin, type I cytoskeletal 14
NCBI Official Synonym Full Names
keratin 14, type I
NCBI Official Symbol
KRT14
NCBI Official Synonym Symbols
K14; NFJ; CK14; EBS3; EBS4
UniProt Protein Name
Keratin, type I cytoskeletal 14
UniProt Synonym Protein Names
Cytokeratin-14; CK-14; Keratin-14; K14
Protein Family
Keratin
UniProt Gene Name
KRT14
UniProt Synonym Gene Names
CK-14; K14
UniProt Entry Name
K1C14_HUMAN
NCBI Protein Information
keratin, type I cytoskeletal 14; CK-14; cytokeratin 14; cytokeratin-14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)

NCBI Summary for KRT14

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]
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UniProt Comments for KRT14

K14: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K5.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: keratin filament; cytoplasm; intermediate filament; cytosol; nucleus

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: hemidesmosome assembly; epidermis development; epithelial cell differentiation; response to zinc ion; response to ionizing radiation; intermediate filament bundle assembly; aging; hair cycle

Disease: Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Simplex, Generalized; Dermatopathia Pigmentosa Reticularis; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Naegeli Syndrome; Epidermolysis Bullosa Simplex, Dowling-meara Type
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Research Articles on KRT14

1. plectin interacts with keratins 5 and 14 in a process associated with epidermolysis bullosa simplex

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KRT14 antibody

 Reference Product  PubMed Publications
 KRT5 antibody  >93 publications with KRT14 and KRT5
 EGFR antibody  >7 publications with KRT14 and EGFR
 DST antibody  >3 publications with KRT14 and DST
 IVL antibody  >1 publications with KRT14 and IVL
 CREBBP antibody  >1 publications with KRT14 and CREBBP

Pathways associated with anti-KRT14 antibody

 Products by Pathway  Pathway Diagram
 Corticotropin-releasing Hormone Pathway antibodies  Corticotropin-releasing Hormone Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram

Diseases associated with anti-KRT14 antibody

 Disease Name  Pubmed Publications
 Epidermolysis Bullosa Simplex Antibodies  >94 publications with KRT14 and Epidermolysis Bullosa Simplex
 Carcinoma Antibodies  >60 publications with KRT14 and Carcinoma
 Carcinoma, Squamous Cell Antibodies  >38 publications with KRT14 and Carcinoma, Squamous Cell
 Disease Models, Animal Antibodies  >23 publications with KRT14 and Disease Models, Animal
 Hyperplasia Antibodies  >21 publications with KRT14 and Hyperplasia
 Breast Neoplasms Antibodies  >18 publications with KRT14 and Breast Neoplasms
 Necrosis Antibodies  >13 publications with KRT14 and Necrosis
 Cell Transformation, Neoplastic Antibodies  >13 publications with KRT14 and Cell Transformation, Neoplastic
 Neoplasms, Experimental Antibodies  >12 publications with KRT14 and Neoplasms, Experimental
 Inflammation Antibodies  >12 publications with KRT14 and Inflammation

Organs/Tissues associated with anti-KRT14 antibody

 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >153 publications with KRT14 and Skin
 Blood Antibodies  >25 publications with KRT14 and Blood
 Mouth Antibodies  >16 publications with KRT14 and Mouth
 Lung Antibodies  >10 publications with KRT14 and Lung
 Eye Antibodies  >8 publications with KRT14 and Eye
 Esophagus Antibodies  >8 publications with KRT14 and Esophagus
 Mammary Gland Antibodies  >7 publications with KRT14 and Mammary Gland
 Connective Tissue Antibodies  >6 publications with KRT14 and Connective Tissue
 Liver Antibodies  >6 publications with KRT14 and Liver
 Cervix Antibodies  >5 publications with KRT14 and Cervix
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