Rat Anti-CD71 Monoclonal Antibody – Cat# MBS213541

CD71 Antibody – Rat Monoclonal

Name: CD71 Antibody; Monoclonal; Rat; MyBioSource
Brand: MyBioSource
SKU: MBS213541
Price: 590 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS213541

Rat anti Mouse CD71 antibody, clone YTA74.4 recognizes the mouse CD71 cell surface molecule, a glycoprotein homodimer of 95kDa subunits. CD71 is expressed by dividing cells, and functions as a transferrin receptor mediating uptake of iron. This clone has been… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

CD71, Monoclonal Antibody

Full Product Name

RAT ANTI MOUSE CD71:RPE

Product Gene Name

anti-CD71 antibody

Clonality

Monoclonal

Isotype

IgG2a

Clone Number

YTA74.4

Host

Rat

Form/Format

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised

Preparation and Storage

Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.

Molecular Weight

85,731 Da

3D Structure

ModBase 3D Structure for Q62351

Reconstitution

Reconstitute with 1 ml distilled water
Perservative Stabilisers||0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen

Concanavilin A activated mouse spleen cells.
Fusion Partners||Spleen cells from an immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
Buffer Solution||Phosphate buffered saline

Target Species

Mouse

Applications Tested/Suitable for anti-CD71 antibody

Flow cytometry (FC/FACS)

Application Notes for anti-CD71 antibody

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Maximum Dilution: Neat

Other Notes

Small volumes of anti-CD71 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD71 purchase

MBS213541 is an antibody targeting CD71. Also known as RAT ANTI MOUSE CD71:RPE. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD71. It may not necessarily be applicable to this product.

NCBI GI #

11596855

NCBI GeneID

22042

NCBI Accession #

NP_035768.1

UniProt Primary Accession #

Q62351

UniProt Secondary Accession #

Q61560

UniProt Related Accession #

Q62351

GenBank Nucleotide #

NM_011638.4

NCBI Official Full Name

transferrin receptor protein 1

NCBI Official Synonym Full Names

transferrin receptor

NCBI Official Symbol

Tfrc

NCBI Official Synonym Symbols

TR; TFR; p90; CD71; TFR1; Trfr; Mtvr1; Mtvr-1; AI195355; AI426448; AU015758; 2610028K12Rik; E430033M20Rik

UniProt Protein Name

Transferrin receptor protein 1

UniProt Synonym Protein Names

CD_antigen: CD71

UniProt Gene Name

Tfrc

UniProt Synonym Gene Names

Trfr; TR; TfR; TfR1; Trfr

UniProt Entry Name

TFR1_MOUSE

NCBI Protein Information

transferrin receptor protein 1; mammary tumor virus receptor 1

NCBI Summary for CD71

This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Mice that are deficient in this receptor show impaired erythroid development and abnormal iron homeostasis. [provided by RefSeq, Mar 2010]

UniProt Comments for CD71

TfR: the transferrin receptor. Regulates the cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied receptors into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system.

Protein type: Cell surface; Receptor, misc.; Membrane protein, integral

Cellular Component: extracellular space; cell surface; mitochondrion; intracellular membrane-bound organelle; cell; recycling endosome membrane; cytoplasmic membrane-bound vesicle; extracellular region; integral to membrane; coated pit; recycling endosome; membrane; perinuclear region of cytoplasm; plasma membrane; endosome; vesicle; external side of plasma membrane

Molecular Function: identical protein binding; iron ion transmembrane transporter activity; protein binding; transferrin receptor activity; chaperone binding; double-stranded RNA binding; Hsp70 protein binding

Biological Process: receptor-mediated endocytosis; cellular iron ion homeostasis; positive regulation of bone resorption; transferrin transport; endocytosis; osteoclast differentiation

Research Articles on CD71

1. high levels of TfRs such as those found on activated lymphocytes were found to be associated with decreased KLRG1 inhibitory function, indicating that TfRs may sequester KLRG1 from interacting with cadherins.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD71 antibody

Products by Pathway	Pathway Diagram
Clathrin Derived Vesicle Budding Pathway antibodies	Clathrin Derived Vesicle Budding Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram
Golgi Associated Vesicle Biogenesis Pathway antibodies	Golgi Associated Vesicle Biogenesis Pathway Diagram
HIF-1 Signaling Pathway antibodies	HIF-1 Signaling Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Iron Uptake And Transport Pathway antibodies	Iron Uptake And Transport Pathway Diagram
Membrane Trafficking Pathway antibodies	Membrane Trafficking Pathway Diagram
Phagosome Pathway antibodies	Phagosome Pathway Diagram

Diseases associated with anti-CD71 antibody

Disease Name	Pubmed Publications
Iron Metabolism Disorders Antibodies	>29 publications with CD71 and Iron Metabolism Disorders
Anemia Antibodies	>21 publications with CD71 and Anemia
Disease Models, Animal Antibodies	>15 publications with CD71 and Disease Models, Animal
Anemia, Iron-Deficiency Antibodies	>11 publications with CD71 and Anemia, Iron-Deficiency
Brain Diseases Antibodies	>10 publications with CD71 and Brain Diseases
Inflammation Antibodies	>9 publications with CD71 and Inflammation
Liver Diseases Antibodies	>8 publications with CD71 and Liver Diseases
Lung Neoplasms Antibodies	>8 publications with CD71 and Lung Neoplasms
Colorectal Neoplasms Antibodies	>6 publications with CD71 and Colorectal Neoplasms
Breast Neoplasms Antibodies	>6 publications with CD71 and Breast Neoplasms

Organs/Tissues associated with anti-CD71 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>45 publications with CD71 and Blood
Liver Antibodies	>28 publications with CD71 and Liver
Brain Antibodies	>25 publications with CD71 and Brain
Lung Antibodies	>14 publications with CD71 and Lung
Intestine Antibodies	>13 publications with CD71 and Intestine
Bone Antibodies	>12 publications with CD71 and Bone
Kidney Antibodies	>10 publications with CD71 and Kidney
Skin Antibodies	>9 publications with CD71 and Skin
Heart Antibodies	>6 publications with CD71 and Heart
Embryonic Tissue Antibodies	>6 publications with CD71 and Embryonic Tissue