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CD22 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS213743

Rat anti Mouse CD22 antibody, clone OX-97 recognizes domain 2 of the murine cell surface glycoprotein CD22, which is a member of the sialoadhesin family. CD22 is expressed on a subpopulation of mature B-lymphocytes and may modulate signalling through the… More details

$605 / 100 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD22, Monoclonal Antibody
Full Product Name
RAT ANTI MOUSE CD22:RPE
Product Gene Name
anti-CD22 antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
OX-97
Host
Rat
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Molecular Weight
92,857 Da
3D Structure
ModBase 3D Structure for P35329
Reconstitution
Reconstitute with 1.0 ml distilled water
Perservative Stabilisers||0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Target Species
Mouse
Applications Tested/Suitable for anti-CD22 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD22 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/5

Testing Data of anti-CD22 antibody

Staining of mouse spleen cells with Rat anti Mouse CD22:FITC (MBS210869)
Rat Anti-CD22 Monoclonal Antibody – Cat# MBS213743

Testing Data of anti-CD22 antibody

Staining of mouse spleen with Rat anti Mouse CD22: Alexa Fluor 647 (MBS212553A647)
Rat Anti-CD22 Monoclonal Antibody, Testing Data – Cat# MBS213743

Other Notes

Small volumes of anti-CD22 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD22 purchase

MBS213743 is an antibody targeting CD22. Also known as RAT ANTI MOUSE CD22:RPE. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD22. It may not necessarily be applicable to this product.

NCBI GI #
112807203
NCBI GeneID
12483
NCBI Accession #
NP_001036782.1
UniProt Primary Accession #
P35329
UniProt Secondary Accession #
Q9JHL2; Q9JJX9; Q9JJY0; Q9JJY1; Q9R056; Q9R094; Q9WU51
UniProt Related Accession #
P35329
GenBank Nucleotide #
NM_001043317.2
NCBI Official Full Name
B-cell receptor CD22
NCBI Official Synonym Full Names
CD22 antigen
NCBI Official Symbol
Cd22
NCBI Official Synonym Symbols
Lyb8; Lyb-8; A530093D23
UniProt Protein Name
B-cell receptor CD22
UniProt Synonym Protein Names
B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; CD_antigen: CD22
Protein Family
B-cell receptor
UniProt Gene Name
Cd22
UniProt Synonym Gene Names
Lyb-8; Siglec2; BL-CAM; Siglec-2
UniProt Entry Name
CD22_MOUSE
NCBI Protein Information
B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; T-cell surface antigen Leu-14; sialic acid-binding Ig-like lectin 2; siglec-2

UniProt Comments for CD22

CD22: Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface

Cellular Component: membrane; integral to plasma membrane; integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; coreceptor activity; carbohydrate binding

Biological Process: cell surface receptor linked signal transduction; cell adhesion
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Research Articles on CD22

1. These data assign a key role to CCL22 in Treg recruitment in the protection of NOD mice against type 1 diabetes following the treatment with G-CSF.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD22 antibody

 Products by Pathway  Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway antibodies  XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway Diagram

Diseases associated with anti-CD22 antibody

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >967 publications with CD22 and Immune System Diseases
 Neoplasms, Experimental Antibodies  >105 publications with CD22 and Neoplasms, Experimental
 Death Antibodies  >64 publications with CD22 and Death
 Inflammation Antibodies  >56 publications with CD22 and Inflammation
 Necrosis Antibodies  >52 publications with CD22 and Necrosis
 Respiratory Tract Diseases Antibodies  >46 publications with CD22 and Respiratory Tract Diseases
 Cell Transformation, Neoplastic Antibodies  >23 publications with CD22 and Cell Transformation, Neoplastic
 Kidney Diseases Antibodies  >22 publications with CD22 and Kidney Diseases
 Liver Diseases Antibodies  >20 publications with CD22 and Liver Diseases
 Lung Diseases Antibodies  >18 publications with CD22 and Lung Diseases

Organs/Tissues associated with anti-CD22 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1059 publications with CD22 and Blood
 Bone Marrow Antibodies  >290 publications with CD22 and Bone Marrow
 Spleen Antibodies  >122 publications with CD22 and Spleen
 Lymph Node Antibodies  >111 publications with CD22 and Lymph Node
 Skin Antibodies  >47 publications with CD22 and Skin
 Embryonic Tissue Antibodies  >13 publications with CD22 and Embryonic Tissue
 Nasopharynx Antibodies  >5 publications with CD22 and Nasopharynx
 Inner Ear Antibodies  >1 publications with CD22 and Inner Ear
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