Rat Anti-CD86 Monoclonal Antibody – Cat# MBS213820

Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD86:FITC (MBS210978)

CD86 Antibody – Rat Monoclonal

Name: CD86 Antibody; Monoclonal; Rat; MyBioSource
Brand: MyBioSource
SKU: MBS213820
Price: 605 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS213820

Rat anti mouse CD86 recognizes mouse CD86 (B7-2), an 80kD cell surface glycoprotein which is a member of the CD28/B7 family. In mouse CD86 is expressed at high levels on peripheral blood monocytes and dendritic cells, and at low levels on resting B and… More details

$605 / 100 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD86, Monoclonal Antibody

Full Product Name

RAT ANTI MOUSE CD86:RPE

Product Gene Name

anti-CD86 antibody

Clonality

Monoclonal

Isotype

IgG2b

Clone Number

PO3

Host

Rat

Form/Format

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised

Preparation and Storage

Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.

Molecular Weight

33,962 Da

3D Structure

ModBase 3D Structure for P42082

Reconstitution

Reconstitute with 1.0 ml distilled water
Perservative Stabilisers||0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen

Mouse B-cell line, BCL1.
Fusion Partners||Spleen cells from immunised Sprague Dawley rats were fused with cells of the P3U1 myeloma cell line.
Buffer Solution||Phosphate buffered saline

Target Species

Mouse

Applications Tested/Suitable for anti-CD86 antibody

Flow cytometry (FC/FACS)

Application Notes for anti-CD86 antibody

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10

Other Notes

Small volumes of anti-CD86 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD86 purchase

MBS213820 is an antibody targeting CD86. Also known as RAT ANTI MOUSE CD86:RPE. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD86. It may not necessarily be applicable to this product.

NCBI GI #

161484599

NCBI GeneID

12524

NCBI Accession #

NP_062261.3

UniProt Primary Accession #

P42082

UniProt Related Accession #

P42082

GenBank Nucleotide #

NM_019388.3

NCBI Official Full Name

T-lymphocyte activation antigen CD86

NCBI Official Synonym Full Names

CD86 antigen

NCBI Official Symbol

Cd86

NCBI Official Synonym Symbols

B7; B70; MB7; B7-2; B7.2; CLS1; Ly58; ETC-1; Ly-58; MB7-2; Cd28l2; TS/A-2

UniProt Protein Name

T-lymphocyte activation antigen CD86

UniProt Synonym Protein Names

Activation B7-2 antigen; Early T-cell costimulatory molecule 1; ETC-1; CD_antigen: CD86

Protein Family

T-lymphocyte activation antigen

UniProt Gene Name

Cd86

UniProt Synonym Gene Names

ETC-1

UniProt Entry Name

CD86_MOUSE

NCBI Protein Information

T-lymphocyte activation antigen CD86; activation B7-2 antigen; early T cell costimulatory molecule-1; early T-cell costimulatory molecule 1

UniProt Comments for CD86

CD86: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Cellular Component: cell surface; intracellular membrane-bound organelle; membrane; integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: receptor binding

Biological Process: B cell activation; negative regulation of T cell anergy; immune system process; toll-like receptor signaling pathway; positive regulation of T cell proliferation; positive regulation of activated T cell proliferation; defense response to virus

Research Articles on CD86

1. data show an important role of the adaptive immune system, in particular T cell CD80/86 costimulation molecules, in the physiological regulation of bone resorption and preservation of bone mass

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD86 antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
CD28 Co-stimulation Pathway antibodies	CD28 Co-stimulation Pathway Diagram
CD28 Dependent PI3K/Akt Signaling Pathway antibodies	CD28 Dependent PI3K/Akt Signaling Pathway Diagram
CD28 Dependent Vav1 Pathway antibodies	CD28 Dependent Vav1 Pathway Diagram
CTLA4 Inhibitory Signaling Pathway antibodies	CTLA4 Inhibitory Signaling Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram

Diseases associated with anti-CD86 antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>1159 publications with CD86 and Necrosis
Inflammation Antibodies	>839 publications with CD86 and Inflammation
Nervous System Diseases Antibodies	>287 publications with CD86 and Nervous System Diseases
Lung Diseases Antibodies	>277 publications with CD86 and Lung Diseases
Neoplasms, Experimental Antibodies	>210 publications with CD86 and Neoplasms, Experimental
Liver Diseases Antibodies	>196 publications with CD86 and Liver Diseases
Cardiovascular Diseases Antibodies	>184 publications with CD86 and Cardiovascular Diseases
Brain Diseases Antibodies	>151 publications with CD86 and Brain Diseases
Dermatitis, Contact Antibodies	>111 publications with CD86 and Dermatitis, Contact
Kidney Diseases Antibodies	>90 publications with CD86 and Kidney Diseases

Organs/Tissues associated with anti-CD86 antibody

Organ/Tissue Name	Pubmed Publications
Bone Marrow Antibodies	>1070 publications with CD86 and Bone Marrow
Spleen Antibodies	>693 publications with CD86 and Spleen
Lymph Node Antibodies	>651 publications with CD86 and Lymph Node
Skin Antibodies	>458 publications with CD86 and Skin
Lung Antibodies	>359 publications with CD86 and Lung
Liver Antibodies	>288 publications with CD86 and Liver
Thymus Antibodies	>122 publications with CD86 and Thymus
Uncharacterized Tissue Antibodies	>3 publications with CD86 and Uncharacterized Tissue
Mammary Gland Antibodies	>1 publications with CD86 and Mammary Gland