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CD25 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS213821

MBS213821 is an antibody targeting CD25. Also known as MOUSE ANTI RAT CD25:RPE. Host: Mouse. More details

$640 / 100 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD25, Monoclonal Antibody
Full Product Name
MOUSE ANTI RAT CD25:RPE
Product Gene Name
anti-CD25 antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
OX-39
Host
Mouse
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Molecular Weight
30,557 Da
Reconstitution
Reconstitute with 1 ml distilled water
Perservative Stabilisers||0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Target Species
Rat
Applications Tested/Suitable for anti-CD25 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD25 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: Neat

Testing Data of anti-CD25 antibody

Staining of Con A activated rat spleen cells with Mouse anti Rat CD25: Biotin (MBS210477)
Mouse Anti-CD25 Monoclonal Antibody – Cat# MBS213821

Testing Data of anti-CD25 antibody

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody , red in A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power
Mouse Anti-CD25 Monoclonal Antibody, Testing Data – Cat# MBS213821

Testing Data of anti-CD25 antibody

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody , red in A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power
Mouse Anti-CD25 Monoclonal Antibody, Testing Data – Cat# MBS213821

Testing Data of anti-CD25 antibody

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. Medium power
Mouse Anti-CD25 Monoclonal Antibody, Testing Data – Cat# MBS213821

Testing Data of anti-CD25 antibody

Staining of stimulated rat spleen cells with Mouse anti Rat CD25:FITC (MBS216315)
Mouse Anti-CD25 Monoclonal Antibody, Testing Data – Cat# MBS213821

Testing Data of anti-CD25 antibody

Published clone specific image: The percentage of Ly49s3+ NK cells increases in the spleen and decreases in the bone marrow following L. monocytogenes infection. A) Mononuclear leukocytes were harvested from spleen, blood, and bone marrow from uninfected and infected animals 24 and 48 hrs p.i. Cells were stained with antibodies to NKR-P1A, CD3, and Ly49s3. NK cells were gated as NKR-P1A+CD3- cells, and the percentage of Ly49s3+ NK cells was analysed by flow cytometry. The data represent the mean +/- SEM from three independent experiments, with 2 animals at each time point. Data were analyzed with the one-way ANOVA with Tukey's multiple comparisons test. B) The activation status of NK cells from uninfected (filled histogram) or infected animals 24 (grey line) and 48 hrs p.i. (black line) was tested by flow cytometry. An antibody towards the activation marker CD25 was employed.From: Shegarfi H, Naper C, Rolstad B, Inngjerdingen M (2010) Listeria monocytogenes Infection Affects a Subset of Ly49-Expressing NK Cells in the Rat. PLoS ONE 5(12): e15579.
Mouse Anti-CD25 Monoclonal Antibody, Testing Data – Cat# MBS213821

Testing Data of anti-CD25 antibody

Staining of rat spleen cells with Mouse anti Rat CD25: FITC (MBS210996)
Mouse Anti-CD25 Monoclonal Antibody, Testing Data – Cat# MBS213821

Testing Data of anti-CD25 antibody

Published customer image: Analysis of graft-infiltrating T cells. Activated CD25+ T cells and CD4+ and CD8+ T cell subsets were stained at the time points of corneal allograft rejection and calculated within the graft. A, C, and E show representative histological staining for CD25, CD4, and CD8 in grafts of treated and control animals, respectively. CD25+ (B) and CD4+ (D) cells infiltrated to a statistically significantly stronger extent in 3.2.3-treated animals when compared to control treated animals (*p
Mouse Anti-CD25 Monoclonal Antibody, Testing Data – Cat# MBS213821

Testing Data of anti-CD25 antibody

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody , red in A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Medium power
Mouse Anti-CD25 Monoclonal Antibody, Testing Data – Cat# MBS213821

Testing Data of anti-CD25 antibody

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. High power
Mouse Anti-CD25 Monoclonal Antibody, Testing Data – Cat# MBS213821

Other Notes

Small volumes of anti-CD25 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD25. It may not necessarily be applicable to this product.

NCBI GI #
7710038
NCBI GeneID
25704
NCBI Accession #
NP_037295.1
UniProt Related Accession #
P26897
GenBank Nucleotide #
NM_013163.1
NCBI Official Full Name
interleukin-2 receptor subunit alpha
NCBI Official Synonym Full Names
interleukin 2 receptor, alpha
NCBI Official Symbol
Il2ra
NCBI Official Synonym Symbols
IL2RAC
UniProt Protein Name
Interleukin-2 receptor subunit alpha
UniProt Synonym Protein Names
CD_antigen: CD25
Protein Family
Interleukin-2 receptor
UniProt Gene Name
Il2ra
UniProt Synonym Gene Names
IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
UniProt Entry Name
IL2RA_RAT
NCBI Protein Information
interleukin-2 receptor subunit alpha; IL-2 receptor alpha subunit; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; interleukin 2 receptor, alpha chain; interleukin-2 receptor alpha chain

NCBI Summary for CD25

alpha chain of the interleukin 2 receptor; human homolog homodimerizes to form low affinity receptor [RGD, Feb 2006]

UniProt Comments for CD25

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

Research Articles on CD25

1. Deletion of antigen-specific T cells by apoptosis and active suppression mediated by CD4(+)CD25(+) T-regulatory cells has an important role in the induction of antigen specific immune tolerance

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD25 antibody

 Reference Product  PubMed Publications
 Il2rg antibody  >116 publications with CD25 and Il2rg
 Il2 antibody  >114 publications with CD25 and Il2
 Jak3 antibody  >25 publications with CD25 and Jak3
 Jak1 antibody  >16 publications with CD25 and Jak1
 Jak2 antibody  >10 publications with CD25 and Jak2
 Tyk2 antibody  >10 publications with CD25 and Tyk2
 Socs3 antibody  >5 publications with CD25 and Socs3
 Syk antibody  >5 publications with CD25 and Syk
 Shc1 antibody  >2 publications with CD25 and Shc1

Pathways associated with anti-CD25 antibody

 Products by Pathway  Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 G Beta:gamma Signalling Through PI3Kgamma Pathway antibodies  G Beta:gamma Signalling Through PI3Kgamma Pathway Diagram
 G-protein Beta:gamma Signalling Pathway antibodies  G-protein Beta:gamma Signalling Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPVI-mediated Activation Cascade Pathway antibodies  GPVI-mediated Activation Cascade Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram

Diseases associated with anti-CD25 antibody

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >1295 publications with CD25 and Immune System Diseases
 Inflammation Antibodies  >541 publications with CD25 and Inflammation
 Necrosis Antibodies  >343 publications with CD25 and Necrosis
 Lung Diseases Antibodies  >198 publications with CD25 and Lung Diseases
 Liver Diseases Antibodies  >159 publications with CD25 and Liver Diseases
 Diabetes Mellitus, Type 1 Antibodies  >149 publications with CD25 and Diabetes Mellitus, Type 1
 Multiple Sclerosis Antibodies  >139 publications with CD25 and Multiple Sclerosis
 Cardiovascular Diseases Antibodies  >128 publications with CD25 and Cardiovascular Diseases
 Arthritis, Rheumatoid Antibodies  >117 publications with CD25 and Arthritis, Rheumatoid
 Kidney Diseases Antibodies  >95 publications with CD25 and Kidney Diseases

Organs/Tissues associated with anti-CD25 antibody

 Organ/Tissue Name  Pubmed Publications
 Mixed Antibodies  >177 publications with CD25 and Mixed
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