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CD45RO Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS213831

MBS213831 is an antibody targeting CD45RO. Also known as MOUSE ANTI HUMAN CD45RO:RPE. Reactive in Human. Host: Mouse. More details

$605 / 100 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD45RO, Monoclonal Antibody
Full Product Name
MOUSE ANTI HUMAN CD45RO:RPE
Product Gene Name
Product Synonym Gene Name
CD45
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
UCHL1
Host
Mouse
Species Reactivity
Human
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Specificity
CD45RO
Preparation and Storage
Prior to reconstitution store at +4 degree C. Following reconstitution store at +4 degree C.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Stability: 12 months from date of reconstitution.
Molecular Weight
147,254 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (198608137..198726545). Location: 1q31-q32
OMIM
126200
3D Structure
ModBase 3D Structure for P08575
Specificity Note
This product recognises the low molecular weight isoform (180kD) of the leucocyte common antigen (LCA). The antigen is expressed by a functional subset of T cell with memory phenotype. In peripheral blood UCHL1 stains 40-80% of lymphocytes, and all monocytes and granulocytes.
Immunogen
Human IL-2 dependent T cells
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse P3/NS1/1-Ag4-1 myeloma cell line.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preservative Stabilisers
0.09% - Sodium Azide
1% - Bovine Serum Albumin
Reconstitution
Reconstitute with 1 ml distilled water
Applications Tested/Suitable for anti-CD45RO antibody
Flow Cytometry
Application Notes for anti-CD45RO antibody
Flow Cytometry: Neat
Flow Cytometry Note: Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Testing Data #1 of anti-CD45RO antibody

Staining of human peripheral blood lymphocytes with Mouse anti Human CD45RO (MBS213021)
Mouse Anti-CD45RO Monoclonal Antibody – Cat# MBS213831

Testing Data #2 of anti-CD45RO antibody

Staining of human peripheral blood lymphocytes with Mouse anti Human CD45RO (MBS213022)
Mouse Anti-CD45RO Monoclonal Antibody, Testing Data #2 – Cat# MBS213831

Testing Data #3 of anti-CD45RO antibody

Staining of human peripheral blood lymphocytes with Mouse anti Human CD45RO:Azide Free
Mouse Anti-CD45RO Monoclonal Antibody, Testing Data #3 – Cat# MBS213831

Product References and Citations for anti-CD45RO antibody

1. Smith, S.H. et al. (1986) Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. Immunology 58: 63-70.
2. Norton, A.J. et al. (1986) Monoclonal antibody (UCHL1) that recognises normal and neoplastic T-cells in routinely fixed tissues. J. Clin. Pathol. 39: 399-405.
3. Beverley, P.C.L. et al. (1986) T-cell subsets and function. Progress in Immunology VI. Cinader, B., Miller, G.G., eds. Academic Press Orlando pp 941-948.
4. Beverley, P.C.L. (1987) Human Tcell subsets. Immunology Letters 14: 263-267.
5. Terry. L.A. et al. (1987) Phenotypic heterogeneity of the CD4+ and CD8+ subsets. Leucocyte Typing III. McMichael, A.J., Beverley, P.C.L. et al. eds. University Press. pp 225-227.
6. Akbar, A.N. et al. (1988) Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T-cells. J. Immunol. 140: 2171 - 2178
7. Terry, L.A. et al. (1988) The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte-common antigen, CD45 Immunology 64: 331-336.
8. Beverley, P.C.L. et al. (1988) Phenotypic diversity of the CD45 antigen and its relation to function. Immunology, Suppl. 1: 3-5.
9. Merkenschlager, M. et al. (1988) Limited dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T-cell memory formation. Eur. J. Immunol. 18: 1653-1661.

Other Notes

Small volumes of anti-CD45RO antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD45RO. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
receptor-type tyrosine-protein phosphatase C isoform 1
NCBI Official Synonym Full Names
protein tyrosine phosphatase, receptor type, C
NCBI Official Symbol
PTPRC
NCBI Official Synonym Symbols
LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase C
UniProt Synonym Protein Names
Leukocyte common antigen; L-CA; T200
UniProt Gene Name
PTPRC
UniProt Synonym Gene Names
CD45
UniProt Entry Name
PTPRC_HUMAN
NCBI Protein Information
receptor-type tyrosine-protein phosphatase C; CD45 antigen; T200 glycoprotein; OTTHUMP00000033813; OTTHUMP00000033816; OTTHUMP00000033817; OTTHUMP00000038574; leukocyte common antigen; leukocyte-common antigen; T200 leukocyte common antigen; protein tyrosine phosphatase, receptor type, c polypeptide

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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