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CD3 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS213843

MBS213843 is an antibody targeting CD3. Also known as RAT ANTI MOUSE CD3:RPE. Host: Rat. More details

$605 / 100 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD3, Monoclonal Antibody
Full Product Name
RAT ANTI MOUSE CD3:RPE
Product Gene Name
anti-CD3 antibody
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
KT3
Host
Rat
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of reconstitution.
Molecular Weight
21,393 Da
Reconstitution
Reconstitute with 1.0 ml distilled water
Perservative Stabilisers||0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
Preparation||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Target Species
Mouse
Applications Tested/Suitable for anti-CD3 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD3 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Maximum Dilution: Neat

Testing Data of anti-CD3 antibody

Staining of mouse spleen with Rat anti Mouse CD3 Epsilon (T3): RPE - Alexa Fluor 647
Rat Anti-CD3 Monoclonal Antibody – Cat# MBS213843

Testing Data of anti-CD3 antibody

Staining of mouse spleen cells with Rat anti Mouse CD3 Epsilon (T3):RPE
Rat Anti-CD3 Monoclonal Antibody, Testing Data – Cat# MBS213843

Testing Data of anti-CD3 antibody

Staining of mouse spleen with Rat anti Mouse CD3 Epsilon (T3): RPE - Alexa Fluor 750
Rat Anti-CD3 Monoclonal Antibody, Testing Data – Cat# MBS213843

Testing Data of anti-CD3 antibody

Immunoperoxidase staining of Mouse lymph node cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195) as a detection reagent. Low power
Rat Anti-CD3 Monoclonal Antibody, Testing Data – Cat# MBS213843

Testing Data of anti-CD3 antibody

Immunoperoxidase staining of Mouse spleen cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195) as a detection reagent. Low power
Rat Anti-CD3 Monoclonal Antibody, Testing Data – Cat# MBS213843

Testing Data of anti-CD3 antibody

Immunoperoxidase staining of Mouse lymph node cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195) as a detection reagent. Low power
Rat Anti-CD3 Monoclonal Antibody, Testing Data – Cat# MBS213843

Testing Data of anti-CD3 antibody

Immunoperoxidase staining of Mouse spleen cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195) as a detection reagent. Medium power
Rat Anti-CD3 Monoclonal Antibody, Testing Data – Cat# MBS213843

Testing Data of anti-CD3 antibody

Immunoperoxidase staining of Mouse lymph node cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195) as a detection reagent. Low power
Rat Anti-CD3 Monoclonal Antibody, Testing Data – Cat# MBS213843

Testing Data of anti-CD3 antibody

Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD3 Epsilon (T3): endotoxin low (MBS224425)
Rat Anti-CD3 Monoclonal Antibody, Testing Data – Cat# MBS213843

Testing Data of anti-CD3 antibody

Staining of mouse spleen cells with Rat anti Mouse CD3 Epsilon (T3): APC (MBS210024)
Rat Anti-CD3 Monoclonal Antibody, Testing Data – Cat# MBS213843

Other Notes

Small volumes of anti-CD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD3. It may not necessarily be applicable to this product.

NCBI GI #
6680886
NCBI GeneID
12501
NCBI Accession #
NP_031674.1
UniProt Related Accession #
P22646
GenBank Nucleotide #
NM_007648.4
NCBI Official Full Name
T-cell surface glycoprotein CD3 epsilon chain
NCBI Official Synonym Full Names
CD3 antigen, epsilon polypeptide
NCBI Official Symbol
Cd3e
NCBI Official Synonym Symbols
CD3; T3e; AI504783; CD3epsilon
UniProt Protein Name
T-cell surface glycoprotein CD3 epsilon chain
UniProt Synonym Protein Names
T-cell surface antigen T3/Leu-4 epsilon chain; CD_antigen: CD3e
Protein Family
CD302 antigen
UniProt Gene Name
Cd3e
UniProt Entry Name
CD3E_MOUSE
NCBI Protein Information
T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain

UniProt Comments for CD3

CD3E: a T cell surface glycoprotein that is a component of the T cell antigen receptor. The recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Contains 1 immunoglobulin-like domain and 1 ITAM domain.

Protein type: Receptor, misc.; Membrane protein, integral

Cellular Component: T cell receptor complex; membrane; integral to membrane; plasma membrane; immunological synapse; alpha-beta T cell receptor complex; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; signal transducer activity; transmembrane receptor activity; protein heterodimerization activity; SH3 domain binding

Biological Process: positive regulation of interleukin-2 biosynthetic process; lymphocyte activation; positive regulation of calcium-mediated signaling; negative thymic T cell selection; T cell receptor signaling pathway; positive regulation of interleukin-4 production; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of interferon-gamma production; cell surface receptor linked signal transduction; negative regulation of smoothened signaling pathway; T cell costimulation; positive regulation of T cell proliferation; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell activation; positive regulation of T cell anergy; response to nutrient
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Research Articles on CD3

1. these results suggest that CD3zeta can be degraded by two pathways: SLAP/c-Cbl, which targets internalized cell surface CD3zeta dependent on TCR signaling, and LAPTM5, which targets intracellular CD3zeta independent of TCR signaling.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD3 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Downstream TCR Signaling Pathway antibodies  Downstream TCR Signaling Pathway Diagram
 Generation Of Second Messenger Molecules Pathway antibodies  Generation Of Second Messenger Molecules Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram

Diseases associated with anti-CD3 antibody

 Disease Name  Pubmed Publications
 Lymphoma Antibodies  >26 publications with CD3 and Lymphoma
 Inflammation Antibodies  >20 publications with CD3 and Inflammation
 Disease Models, Animal Antibodies  >16 publications with CD3 and Disease Models, Animal
 Immunologic Deficiency Syndromes Antibodies  >14 publications with CD3 and Immunologic Deficiency Syndromes
 Necrosis Antibodies  >13 publications with CD3 and Necrosis
 Neoplasms, Experimental Antibodies  >7 publications with CD3 and Neoplasms, Experimental
 Severe Combined Immunodeficiency Antibodies  >5 publications with CD3 and Severe Combined Immunodeficiency
 Carcinoma Antibodies  >5 publications with CD3 and Carcinoma
 Lung Diseases Antibodies  >5 publications with CD3 and Lung Diseases
 Arthritis, Rheumatoid Antibodies  >5 publications with CD3 and Arthritis, Rheumatoid

Organs/Tissues associated with anti-CD3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >190 publications with CD3 and Blood
 Thymus Antibodies  >46 publications with CD3 and Thymus
 Spleen Antibodies  >35 publications with CD3 and Spleen
 Bone Marrow Antibodies  >26 publications with CD3 and Bone Marrow
 Mammary Gland Antibodies  >1 publications with CD3 and Mammary Gland
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