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CD28 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS213865

Rat anti Human CD28 antibody, clone YTH913.12 recognizes human CD28, a ~44kDa single pass type 1 trans-membrane protein expressed as a homodimer on a major subset of human T-cells (Thompson et al. 1989), responsible for activation of these cells via… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CD28, Monoclonal Antibody
Full Product Name
RAT ANTI HUMAN CD28:RPE
Product Gene Name
anti-CD28 antibody
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
YTH913.12
Host
Rat
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Molecular Weight
26,186 Da
OMIM
186760
3D Structure
ModBase 3D Structure for P10747
Reconstitution
Reconstitute with 1 ml distilled water
Perservative Stabilisers||0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Target Species
Human
Applications Tested/Suitable for anti-CD28 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD28 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul human whole blood.
Flow Cytometry: Maximum Dilution: Neat

Testing Data of anti-CD28 antibody

Staining of human peripheral blood lymphocytes with Rat anti Human CD28: Biotin (MBS210509)
Rat Anti-CD28 Monoclonal Antibody – Cat# MBS213865

Testing Data of anti-CD28 antibody

Staining of human peripheral blood lymphocytes with Rat anti Human CD28:RPE
Rat Anti-CD28 Monoclonal Antibody, Testing Data – Cat# MBS213865

Testing Data of anti-CD28 antibody

Staining of human peripheral blood lymphocytes with Rat anti Human CD28: Alexa Fluor 647 (MBS2141859A647)
Rat Anti-CD28 Monoclonal Antibody, Testing Data – Cat# MBS213865

Testing Data of anti-CD28 antibody

Staining of human peripheral blood lymphocytes with Rat anti Human CD28 (MBS213120)
Rat Anti-CD28 Monoclonal Antibody, Testing Data – Cat# MBS213865

Other Notes

Small volumes of anti-CD28 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD28 purchase

MBS213865 is an antibody targeting CD28. Also known as RAT ANTI HUMAN CD28:RPE. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD28. It may not necessarily be applicable to this product.

NCBI GI #
340545508
NCBI GeneID
940
NCBI Accession #
NP_001230006.1
UniProt Primary Accession #
P10747
UniProt Secondary Accession #
Q13964; Q52M23; Q70WG0; Q8NI54; Q8NI55; Q8NI56; Q8WXJ2; Q9BYV0; A8KAC1
UniProt Related Accession #
P10747
GenBank Nucleotide #
NM_001243077.1
NCBI Official Full Name
T-cell-specific surface glycoprotein CD28 isoform 2
NCBI Official Synonym Full Names
CD28 molecule
NCBI Official Symbol
CD28
NCBI Official Synonym Symbols
Tp44
UniProt Protein Name
T-cell-specific surface glycoprotein CD28
UniProt Synonym Protein Names
TP44; CD_antigen: CD28
Protein Family
T-cell-specific surface glycoprotein
UniProt Gene Name
CD28
UniProt Entry Name
CD28_HUMAN
NCBI Protein Information
T-cell-specific surface glycoprotein CD28; CD28 antigen

NCBI Summary for CD28

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

UniProt Comments for CD28

CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: integral to plasma membrane; plasma membrane; immunological synapse; cytosol; external side of plasma membrane

Molecular Function: identical protein binding; protein binding; protease binding; SH3/SH2 adaptor activity; coreceptor activity

Biological Process: positive regulation of isotype switching to IgG isotypes; viral reproduction; nerve growth factor receptor signaling pathway; positive regulation of translation; cytokine biosynthetic process; positive regulation of interleukin-2 biosynthetic process; T cell receptor signaling pathway; positive regulation of inflammatory response to antigenic stimulus; cell surface receptor linked signal transduction; positive regulation of T cell proliferation; regulatory T cell differentiation; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of viral genome replication; positive regulation of mitosis; negative thymic T cell selection; regulation of defense response to virus by virus; humoral immune response; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; T cell costimulation; innate immune response; positive regulation of alpha-beta T cell proliferation; positive regulation of transcription from RNA polymerase II promoter
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Research Articles on CD28

1. Studies suggest that CD28 T > C polymorphism (rs3116496) may have an increased risk of cancer in Asians.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD28 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 CD28 Co-stimulation Pathway antibodies  CD28 Co-stimulation Pathway Diagram
 CD28 Dependent PI3K/Akt Signaling Pathway antibodies  CD28 Dependent PI3K/Akt Signaling Pathway Diagram
 CD28 Dependent Vav1 Pathway antibodies  CD28 Dependent Vav1 Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram

Diseases associated with anti-CD28 antibody

 Disease Name  Pubmed Publications
 Autoimmune Diseases Antibodies  >1237 publications with CD28 and Autoimmune Diseases
 Necrosis Antibodies  >849 publications with CD28 and Necrosis
 Inflammation Antibodies  >806 publications with CD28 and Inflammation
 HIV Infections Antibodies  >453 publications with CD28 and HIV Infections
 Arthritis, Rheumatoid Antibodies  >358 publications with CD28 and Arthritis, Rheumatoid
 Lung Diseases Antibodies  >313 publications with CD28 and Lung Diseases
 Lymphoma Antibodies  >282 publications with CD28 and Lymphoma
 Carcinoma Antibodies  >233 publications with CD28 and Carcinoma
 Asthma Antibodies  >199 publications with CD28 and Asthma
 Liver Diseases Antibodies  >163 publications with CD28 and Liver Diseases

Organs/Tissues associated with anti-CD28 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >8126 publications with CD28 and Blood
 Thymus Antibodies  >343 publications with CD28 and Thymus
 Brain Antibodies  >121 publications with CD28 and Brain
 Embryonic Tissue Antibodies  >56 publications with CD28 and Embryonic Tissue
 Pharynx Antibodies  >34 publications with CD28 and Pharynx
 Connective Tissue Antibodies  >24 publications with CD28 and Connective Tissue
 Placenta Antibodies  >19 publications with CD28 and Placenta
 Uterus Antibodies  >19 publications with CD28 and Uterus
 Trachea Antibodies  >11 publications with CD28 and Trachea
 Adipose Tissue Antibodies  >7 publications with CD28 and Adipose Tissue
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