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CD11b Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS213874

MBS213874 is an antibody targeting CD11b. Also known as RAT ANTI MOUSE CD11b:RPE. Host: Rat. More details

$605 / 100 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD11b, Monoclonal Antibody
Full Product Name
RAT ANTI MOUSE CD11b:RPE
Product Gene Name
anti-CD11b antibody
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
M1/70.15
Host
Rat
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Molecular Weight
115,196 Da
Reconstitution
Reconstitute with 1 ml distilled water
Perservative Stabilisers||0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
T cells enriched splenocytes from B10 mice.
Fusion Partners||Spleen cells from an immunised DA rat were fused with cells of the mouse NS1/1.Ag4.1 myeloma cell line.
Buffer Solution||Phosphate buffered saline
Target Species
Mouse
Applications Tested/Suitable for anti-CD11b antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD11b antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10

Testing Data of anti-CD11b antibody

Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195). Medium power
Rat Anti-CD11b Monoclonal Antibody – Cat# MBS213874

Testing Data of anti-CD11b antibody

Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b: Alexa Fluor 647
Rat Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS213874

Testing Data of anti-CD11b antibody

Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195). High power
Rat Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS213874

Testing Data of anti-CD11b antibody

Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD11b:FITC (MBS211086)
Rat Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS213874

Testing Data of anti-CD11b antibody

Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b:RPE (MBS213875)
Rat Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS213874

Testing Data of anti-CD11b antibody

Published customer image: Immunocytochemical characterisation of cultures of mouse microglia. Cultures of microglia were immunostained with anti-CD11b (a) and anti-GFAP (c). Cultures of astrocytes were immunostained with anti-CD11b (c) and anti-GFAP (d). All cells were also counterstained with DAPI (blue) to identify the cells' nuclei.From: Ferger et al. Journal of Neuroinflammation 2010 7:45
Rat Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS213874

Testing Data of anti-CD11b antibody

Staining of total mouse peritoneal exudate cells demonstrating labelling of macrophages with Rat anti Mouse CD11b:RPE
Rat Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS213874

Testing Data of anti-CD11b antibody

Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD11b:Alexa Fluor 488
Rat Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS213874

Testing Data of anti-CD11b antibody

Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b (MBS213144)
Rat Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS213874

Testing Data of anti-CD11b antibody

Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b (MBS213143)
Rat Anti-CD11b Monoclonal Antibody, Testing Data – Cat# MBS213874

Other Notes

Small volumes of anti-CD11b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD11b. It may not necessarily be applicable to this product.

NCBI GI #
124956
NCBI GeneID
16409
NCBI Accession #
P05555.2
UniProt Secondary Accession #
Q8CA73
UniProt Related Accession #
P05555
NCBI Official Full Name
Integrin alpha-M
NCBI Official Synonym Full Names
integrin alpha M
NCBI Official Symbol
Itgam
NCBI Official Synonym Symbols
CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
UniProt Protein Name
Integrin alpha-M
UniProt Synonym Protein Names
CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; CD_antigen: CD11b
Protein Family
Integrin
UniProt Gene Name
Itgam
UniProt Entry Name
ITAM_MOUSE
NCBI Protein Information
integrin alpha-M; CD11 antigen-like family member B; CD11B (p170); CR-3 alpha chain; Mac-1 alpha; cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component receptor 3 alpha-a; complement receptor type 3; leukocyte adhesion receptor MO1; macrophage antigen alpha

UniProt Comments for CD11b

ITGAM: a single-pass type I membrane protein of the integrin alpha chain family. Implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. Also known as CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. A receptor for fibrinogen, factor X and ICAM1. Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2. Predominantly expressed in monocytes and granulocytes.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; nucleus; integrin complex; external side of plasma membrane

Molecular Function: heparin binding; heparan sulfate proteoglycan binding; opsonin binding; protein heterodimerization activity; metal ion binding; glycoprotein binding

Biological Process: integrin-mediated signaling pathway; neutrophil chemotaxis; cell-cell adhesion; leukocyte adhesion; microglia development; cellular extravasation; activated T cell proliferation; cell adhesion; leukocyte migration during inflammatory response
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Research Articles on CD11b

1. These findings indicate that inhibition of indoleamine 2,3-dioxygenase-1 reduces mortality from peritonitis and sepsis after CLP via recruitment of neutrophils and mononuclear cells by chemokine production in peritoneal CD11b(+) cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD11b antibody

 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram

Organs/Tissues associated with anti-CD11b antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >4528 publications with CD11b and Blood
 Bone Marrow Antibodies  >798 publications with CD11b and Bone Marrow
 Brain Antibodies  >631 publications with CD11b and Brain
 Lung Antibodies  >545 publications with CD11b and Lung
 Spleen Antibodies  >519 publications with CD11b and Spleen
 Skin Antibodies  >248 publications with CD11b and Skin
 Heart Antibodies  >211 publications with CD11b and Heart
 Spinal Cord Antibodies  >164 publications with CD11b and Spinal Cord
 Intestine Antibodies  >161 publications with CD11b and Intestine
 Eye Antibodies  >152 publications with CD11b and Eye
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