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Phenylalanine Hydroxylase (PAH) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2139179

MBS2139179 is an antibody targeting PAH. Also known as Monoclonal Antibody to Phenylalanine Hydroxylase (PAH). Reactive in Human, Pig. Host: Mouse. More details

$225 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Phenylalanine Hydroxylase (PAH), Monoclonal Antibody
Full Product Name
Monoclonal Antibody to Phenylalanine Hydroxylase (PAH)
Product Gene Name
Product Synonym Names
PAH/Phenylalanine Hydroxylase; PKU; PKU1; Phe4monooxygenase
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
C10
Host
Mouse
Species Reactivity
Human, Pig
Form/Format
Liquid; 0.01M PBS, pH7.4, containing 0.05% Proclin300, 50% glycerol.
Purity/Purification
Protein A + Protein G affinity chromatography
Concentration
1mg/mL (lot specific)
Specificity
The antibody is a mouse monoclonal antibody raised against PAH. It has been selected for its ability to recognize PAH in immunohistochemical staining and western blotting.
Preparation and Storage
Store at 2 degree C to 8 degree C for frequent use, 20 degree C for 24 months
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: PAH Clone #C10 (MBS2139179)
APC-CY7 Conjugated Antibody: PAH Clone #C10 (MBS2135363)
Matching Pairs
Unconjugated Antibody: PAH Clone #C10 (MBS2139179)
PE Conjugated Antibody: PAH Clone #C10 (MBS2135365)
Molecular Weight
452
OMIM
phenotype 612349
3D Structure
ModBase 3D Structure for P00439
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen Type
Prokaryotic Recombinant proteins/RP
Sequence of Immunogen
Glu228~Lys452
Conjugation
Unconjugated

Product Categories/Family for anti-PAH antibody

ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-PAH antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes for anti-PAH antibody
WB: 0.52ug/mL
IHC: 520ug/mL
ICC: 520ug/mL

Immunohistochemistry (IHC) of anti-PAH antibody

DAB staining on IHCP; Sample: Human Kidney Tissue; Primary Ab: 20ug/ml Mouse AntiHuman PAH Antibody Second Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Mouse Anti-PAH Monoclonal Antibody – Cat# MBS2139179

Other Notes

Small volumes of anti-PAH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PAH. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Phenylalanine-4-hydroxylase
NCBI Official Synonym Full Names
phenylalanine hydroxylase
NCBI Official Symbol
PAH
NCBI Official Synonym Symbols
PH; PKU; PKU1
UniProt Protein Name
Phenylalanine-4-hydroxylase
UniProt Synonym Protein Names
Phe-4-monooxygenase
UniProt Gene Name
PAH
UniProt Synonym Gene Names
PAH
UniProt Entry Name
PH4H_HUMAN
NCBI Protein Information
phenylalanine-4-hydroxylase; phe-4-monooxygenase; phenylalanine 4-monooxygenase

NCBI Summary for PAH

PAH encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria. [provided by RefSeq, Jul 2008]

UniProt Comments for PAH

PAH: phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.

Protein type: EC 1.14.16.1; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Oxidoreductase

Chromosomal Location of Human Ortholog: 12q22-q24.2

Cellular Component: cytosol

Molecular Function: amino acid binding; iron ion binding; phenylalanine 4-monooxygenase activity

Biological Process: L-phenylalanine catabolic process; catecholamine biosynthetic process; amino acid biosynthetic process; neurotransmitter biosynthetic process

Disease: Phenylketonuria
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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