• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Substance P (TAC1) Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS213959

Rat anti substance P antibody, clone NC1/34 recognizes the COOH terminal end of substance P, a short polypeptide neurotransmitter that regulates the excitability of dorsal horn nociceptive neurons. It is known to have a role in several physiologic activities… More details

$635 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SUBSTANCE P (TAC1), Monoclonal Antibody
Full Product Name
RAT ANTI SUBSTANCE P
Product Gene Name
Product Synonym Names
NKA, NKNA, TAC2
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
NC1/34
Host
Rat
Form/Format
Tissue Culture Supernatant - liquid
Specificity
SUBSTANCE P
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
11,376 Da
OMIM
162320
3D Structure
ModBase 3D Structure for P20366
Species Cross Reactivity
Reacts with: Pig, Pigeon, Bichir, Rabbit, Human, Bovine, Guinea Pig, Calanus sp., Cancer sp., Panilurus sp., Homarus sp., Mudpuppy, Lamprey, Crayfish, Zebrafish, Bullfrog, Cockroach, Sheep, Cowbird, Rat, Spadefoot toad, Chicken, Chinchilla, Camel.

Based on sequence similarity, is expected to react with: Mammals, Crustacea, Amphibia
N.B. Antibody reactivity and working conditions may vary between species.
Perservative Stabilisers
0.05% Thiomersal
Immunogen
Substance P conjugated to Bovine Serum Albumin
Fusion Partners
Spleen cells from immunised wistar rat were fused with cells of the Ns1/1-Ag 4-1 myeloma cell line.
Applications Tested/Suitable for anti-TAC1 antibody
Immunohistology Frozen, Immunofluorescence (IF), Immunohistology Paraffin
Application Notes for anti-TAC1 antibody
Immunohistology - Frozen: Minimum Dilution: 1/100; Maximum Dilution: 1/200;
Immunohistology - Paraffin: Minimum Dilution: 1/100; Maximum Dilution: 1/200; Application Note: This product does not require antigen retrieval prior to staining of formalin fixed, paraffin embedded sections.

Testing Data of anti-TAC1 antibody

Published customer image: A,B. Tachykinin-like immunoreactivity in cells in inflammatory infiltrate of myositis area. Sections of a specimen from a soleus muscle specimen of the 6w group, non-exercised side. In (A), the section had been immunostained with MBS213959, and in (B), the section was immunostained with MBS213959 after preabsorption with tachykinin (SP) (larger area is covered in B than in A). Immunoreactivity is seen in cells in (A), but no reaction is visible in (B). Arrows point at cells of inflammatory infiltrate. The immunoreactions show partly a granular appearance (A). Magnification x315. c,d. Immunostaining with polyclonal and monoclonal tachykinin antibodies. A part of a focal myositis area of a section of a gastrocnemius muscle specimen from the 1w group, exercised side, double-stained for tachykinin using the sc-14104 (C) and MBS213959 (D) antibodies, shown at low magnification. Note that the cells of an inflammatory infiltrate are immunolabelled in both (C) and (D) (arrows), whereas the wall of a small vessel is only immunostained in (C) (asterisks). Magnification x315.From: Song Y, St¥l PS, Yu J-G, Forsgren S. Bilateral increase in expression and concentration of tachykinin in a unilateral rabbit muscle overuse model that leads to myositis. BMC Musculoskelet Disord [Internet]. Springer Science + Business Media; 2013;14(1):134.
Rat Anti-TAC1 Monoclonal Antibody – Cat# MBS213959

Testing Data of anti-TAC1 antibody

Published customer image: Tachykinin-like immunoreactivity in nerve fascicles. Nerve fascicles and parts of nerve fascicles (N) of sections from gastrocnemius (A-C, E, F) and soleus (D) muscle specimens are shown. The sections were processed for demonstration of tachykinin-like immunoreaction (A-E). In (F) the reaction pattern after preabsorption with SP peptide is shown (the region in F corresponds to the region in E). The antibodies applied were sc-14104 (A-C, E) and MBS213959 (D). The specimens were from the control group (A, B, E, F) and from the 6w group, non-exercised side, focal myositis area (C, D). There are immunoreactivities in nerve fascicles in the form of punctuate reactions (arrows A, C-E). These are grouped, as especially seen in (C), but also to some extent in (D). There are no reactions in the nerve fascicle in (B), which is built up of myelinated nerve fibers, and in the control section in (F). MF refers to muscle fibers. Magnification x200 (B, C), x315 (A, D, E, F).From: Song Y, St¥l PS, Yu J-G, Forsgren S. Bilateral increase in expression and concentration of tachykinin in a unilateral rabbit muscle overuse model that leads to myositis. BMC Musculoskelet Disord [Internet]. Springer Science + Business Media; 2013;14(1):134.
Rat Anti-TAC1 Monoclonal Antibody, Testing Data – Cat# MBS213959

Other Notes

Small volumes of anti-TAC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TAC1 purchase

MBS213959 is an antibody targeting TAC1. Also known as RAT ANTI SUBSTANCE P. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for TAC1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protachykinin-1 isoform beta
NCBI Official Synonym Full Names
tachykinin, precursor 1
NCBI Official Symbol
TAC1
NCBI Official Synonym Symbols
NK2; NPK; NKNA; TAC2; Hs.2563
UniProt Protein Name
Protachykinin-1
UniProt Synonym Protein Names
PPTCleaved into the following 5 chains:Substance P; Neurokinin A; NKAAlternative name(s):Neuromedin L; Substance K
Protein Family
UniProt Gene Name
TAC1
UniProt Synonym Gene Names
NKA; NKNA; TAC2; NKA; NPK
UniProt Entry Name
TKN1_HUMAN
NCBI Protein Information
protachykinin-1; PPT; neurokinin 1; neurokinin 2; neurokinin A; neurokinin alpha; neuromedin L; neuropeptide K; neuropeptide gamma; preprotachykinin; substance K; substance P; tachykinin 2; tachykinin, precursor 1 (substance K, substance P, neurokinin 1, neurokinin 2, neuromedin L, neurokinin alpha, neuropeptide K, neuropeptide gamma)

NCBI Summary for TAC1

This gene encodes four products of the tachykinin peptide hormone family, substance P and neurokinin A, as well as the related peptides, neuropeptide K and neuropeptide gamma. These hormones are thought to function as neurotransmitters which interact with nerve receptors and smooth muscle cells. They are known to induce behavioral responses and function as vasodilators and secretagogues. Substance P is an antimicrobial peptide with antibacterial and antifungal properties. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]
Show more

UniProt Comments for TAC1

TAC1: Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. Belongs to the tachykinin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q21-q22

Cellular Component: extracellular space; cell soma; axon; extracellular region; plasma membrane

Molecular Function: protein binding; substance P receptor binding

Biological Process: tachykinin signaling pathway; negative regulation of heart rate; insemination; response to hormone stimulus; response to morphine; response to lipopolysaccharide; response to pain; sensory perception of pain; positive regulation of saliva secretion; synaptic transmission; elevation of cytosolic calcium ion concentration; cell-cell signaling; positive regulation of acute inflammatory response; positive regulation of lymphocyte proliferation; neuropeptide signaling pathway; regulation of blood pressure; positive regulation of stress fiber formation; positive regulation of action potential; inflammatory response; detection of abiotic stimulus; associative learning; positive regulation of synaptic transmission, cholinergic; positive regulation of synaptic transmission, GABAergic; positive regulation of ossification; long-term memory
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000