CD117 Antibody – Rat Monoclonal

The c-Kit proto-oncogene is the cellular homolog of the transforming gene of a feline retrovirus (v-Kit). The c-kit protein includes characteristics of a protein kinase transmembrane receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Kit: a receptor tyrosine kinase and a member of the subfamily that includes PDGF, CSF-1 and FLT-3/flk-2 receptors. Receptor for stem cell factor. Plays a critical role in hematopoietic stem cell, mast cell, melanocyte and germ cell development. Ligand binding induces autophosphorylation, dimerization and activation, leading to the recruitment and phosphorylation of downstream SH2-containing signaling components including PLC-gamma, PI3 kinase p85, SHP2 and CrkL, linking c-Kit to various cell signaling pathways. Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders, while mutations that constitutively activate c-Kit can lead to hyperplasia and tumorigenesis. Activating mutations cause >90% of gastrointestinal stromal tumors (GIST); successfully treated with inhibitors Gleevec (imatinib, Glivec) and Sutent (Sutinib, SU11248). Activating mutations also induce mastocytosis. Autocrine/paracrine stimulation may drive some lung and other tumors. Loss of expression associated with melanoma progression. Familial loss of function mutations cause piebaldism, with defects in hair and skin pigmentation due to lack of melanocytes.

Protein type: Protein kinase, TK; Oncoprotein; Protein kinase, tyrosine (receptor); Membrane protein, integral; EC 2.7.10.1; Kinase, protein; TK group; PDGFR family

Cellular Component: extracellular space; internal side of plasma membrane; cell surface; mast cell granule; membrane; cytoplasm; plasma membrane; acrosome; integral to membrane; intercellular junction; intracellular; external side of plasma membrane

Molecular Function: protein homodimerization activity; protease binding; metal ion binding; nucleotide binding; stem cell factor receptor activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; protein kinase activity; transferase activity; protein binding; cytokine binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; kinase activity; ATP binding

Biological Process: activation of MAPK activity; somatic stem cell maintenance; germ cell programmed cell death; positive regulation of JAK-STAT cascade; lymphoid progenitor cell differentiation; positive regulation of long-term neuronal synaptic plasticity; protein amino acid phosphorylation; germ cell development; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of MAP kinase activity; regulation of cell shape; epithelial cell proliferation; germ cell migration; somatic stem cell division; erythrocyte differentiation; T cell differentiation; mast cell chemotaxis; embryonic hemopoiesis; stem cell differentiation; detection of mechanical stimulus involved in sensory perception of sound; positive regulation of tyrosine phosphorylation of Stat1 protein; immature B cell differentiation; glycosphingolipid metabolic process; regulation of pigmentation during development; positive regulation of transcription factor activity; mast cell cytokine production; transmembrane receptor protein tyrosine kinase signaling pathway; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; pigmentation during development; chemotaxis; response to radiation; myeloid progenitor cell differentiation; ovarian follicle development; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; melanocyte differentiation; negative regulation of programmed cell death; visual learning; hemopoiesis; cell differentiation; inflammatory response; positive regulation of Notch signaling pathway; lamellipodium biogenesis; dendritic cell cytokine production; cytokine and chemokine mediated signaling pathway; mast cell degranulation; positive regulation of pseudopodium formation; positive regulation of tyrosine phosphorylation of Stat5 protein; pigmentation; gut development; actin cytoskeleton reorganization; myeloid leukocyte differentiation; spermatogenesis; phosphorylation; spermatid development; positive regulation of cell migration