Mouse Anti-CDw328 Monoclonal Antibody – Cat# MBS214368

Peripheral Human Monocytes stained with Mouse anti Human CDw328:RPE (MBS216227)

CDw328 Antibody – Mouse Monoclonal

Name: CDw328 Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS214368
Price: 565 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS214368

Mouse anti Human CDw328 antibody, clone 5-386 recognizes human CDw328, a type I transmembrane glycoprotein and member of the Siglec (sialic acid binding Ig-like lectin) family, designated Siglec-7, originally identified as an inhibitory NK cell receptor (NKR)… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

CDw328, Monoclonal Antibody

Full Product Name

MOUSE ANTI HUMAN CDw328

Product Gene Name

anti-CDw328 antibody

Clonality

Monoclonal

Isotype

IgG1

Clone Number

5-386

Host

Mouse

Form/Format

Purified
Purified IgG - liquid

Concentration

IgG concentration 1.0mg/ml (lot specific)

Preparation and Storage

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Molecular Weight

16,740 Da

OMIM

604410

3D Structure

ModBase 3D Structure for Q9Y286

Perservative Stabilisers

0.09% Sodium Azide (NaN3)
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen

Monocyte-derived dendritic cells
Histology Positive Control Tissue||Human tonsil.
Fusion Partners||Spleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Buffer Solution||Phosphate buffered saline

Target Species

Human

Applications Tested/Suitable for anti-CDw328 antibody

Immunohistology Frozen, Flow cytometry (FC/FACS)

Application Notes for anti-CDw328 antibody

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Immunohistology - Frozen: Maximum Dilution: 1/100
Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200

Other Notes

Small volumes of anti-CDw328 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDw328 purchase

MBS214368 is an antibody targeting CDw328. Also known as MOUSE ANTI HUMAN CDw328. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CDw328. It may not necessarily be applicable to this product.

NCBI GI #

469469047

NCBI GeneID

27036

NCBI Accession #

NP_001264130.1

UniProt Primary Accession #

Q9Y286

UniProt Secondary Accession #

Q9NZQ1; Q9UJ86; Q9UJ87; Q9Y502

UniProt Related Accession #

Q9Y286

GenBank Nucleotide #

NM_001277201.1

NCBI Official Full Name

sialic acid-binding Ig-like lectin 7 isoform 3

NCBI Official Synonym Full Names

sialic acid binding Ig-like lectin 7

NCBI Official Symbol

SIGLEC7

NCBI Official Synonym Symbols

p75; QA79; AIRM1; CD328; CDw328; D-siglec; SIGLEC-7; SIGLECP2; SIGLEC19P; p75/AIRM1

UniProt Protein Name

Sialic acid-binding Ig-like lectin 7

UniProt Synonym Protein Names

Adhesion inhibitory receptor molecule 1; AIRM-1; CDw328; D-siglec; QA79 membrane protein; p75; CD_antigen: CD328

Protein Family

Sialic acid-binding Ig-like lectin

UniProt Gene Name

SIGLEC7

UniProt Synonym Gene Names

AIRM1; Siglec-7; AIRM-1

UniProt Entry Name

SIGL7_HUMAN

NCBI Protein Information

sialic acid-binding Ig-like lectin 7; AIRM-1; QA79 membrane protein; adhesion inhibitory receptor molecule 1, siglec-7; sialic acid binding immunoglobulin-like lectin 7

UniProt Comments for CDw328

SIGLEC7: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: integral to plasma membrane

Molecular Function: receptor activity; carbohydrate binding

Biological Process: cell adhesion

Research Articles on CDw328

1. Siglec-7 plays a significant role in inhibiting IgE-mediated mast cell activation, but only moderately affects IgE-mediated activation in primary human basophils.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CDw328 antibody

Reference Product	PubMed Publications
PTPN11 antibody	>3 publications with CDw328 and PTPN11
SYK antibody	>1 publications with CDw328 and SYK

Diseases associated with anti-CDw328 antibody

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>4 publications with CDw328 and Kidney Diseases
Lung Neoplasms Antibodies	>2 publications with CDw328 and Lung Neoplasms
Nervous System Diseases Antibodies	>2 publications with CDw328 and Nervous System Diseases
Colorectal Neoplasms Antibodies	>2 publications with CDw328 and Colorectal Neoplasms
Peripheral Nervous System Diseases Antibodies	>1 publications with CDw328 and Peripheral Nervous System Diseases
Cell Transformation, Neoplastic Antibodies	>1 publications with CDw328 and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-CDw328 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>31 publications with CDw328 and Blood
Liver Antibodies	>2 publications with CDw328 and Liver
Spleen Antibodies	>2 publications with CDw328 and Spleen
Eye Antibodies	>1 publications with CDw328 and Eye
Adrenal Gland Antibodies	>1 publications with CDw328 and Adrenal Gland