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CD16 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS214375

Mouse anti Human CD16 antibody, clone DJ130c recognizes human CD16, also known as Low affinity immunoglobulin gamma Fc region receptor III-A or Fc-gamma RIIIa. CD16a is a 254 amino acid ~50-65kDa single pass type 1 transmembrane glycoprotein bearing two… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CD16, Monoclonal Antibody
Full Product Name
MOUSE ANTI HUMAN CD16:RPE
Product Gene Name
anti-CD16 antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
DJ130c
Host
Mouse
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of reconstitution.
Molecular Weight
26,216 Da
OMIM
610665
Reconstitution
Reconstitute with 1.0ml distilled water
Perservative Stabilisers||0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Target Species
Human
Applications Tested/Suitable for anti-CD16 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD16 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10

Testing Data of anti-CD16 antibody

Staining of human peripheral blood lymphocytes with Mouse anti Human CD16 (MBS216231)
Mouse Anti-CD16 Monoclonal Antibody – Cat# MBS214375

Testing Data of anti-CD16 antibody

Staining of human peripheral blood lymphocytes with Mouse anti Human CD16:Alexa Fluor 647 (MBS214373A647T)
Mouse Anti-CD16 Monoclonal Antibody, Testing Data – Cat# MBS214375

Other Notes

Small volumes of anti-CD16 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD16 purchase

MBS214375 is an antibody targeting CD16. Also known as MOUSE ANTI HUMAN CD16:RPE. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD16. It may not necessarily be applicable to this product.

NCBI GI #
132814489
NCBI GeneID
2215
NCBI Accession #
NP_000561.3
UniProt Related Accession #
O75015
GenBank Nucleotide #
NM_000570.4
NCBI Official Full Name
low affinity immunoglobulin gamma Fc region receptor III-B isoform 2
NCBI Official Synonym Full Names
Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
NCBI Official Symbol
FCGR3B
NCBI Official Synonym Symbols
CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor III-B
UniProt Synonym Protein Names
Fc-gamma RIII-beta; Fc-gamma RIII; Fc-gamma RIIIb; FcRIII; FcRIIIb; FcR-10; IgG Fc receptor III-1; CD_antigen: CD16b
Protein Family
CD160 antigen
UniProt Gene Name
FCGR3B
UniProt Synonym Gene Names
CD16B; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIb; FcRIII; FcRIIIb
UniProt Entry Name
FCG3B_HUMAN
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor III-B; Fc-gamma receptor IIIb (CD 16); fc-gamma RIII-beta; fc-gamma RIIIb; igG Fc receptor III-1

NCBI Summary for CD16

The protein encoded by this gene is a low affinity receptor for the Fc region of gamma immunoglobulins (IgG). The encoded protein acts as a monomer and can bind either monomeric or aggregated IgG. This gene may function to capture immune complexes in the peripheral circulation. Several transcript variants encoding different isoforms have been found for this gene. A highly-similar gene encoding a related protein is also found on chromosome 1. [provided by RefSeq, Aug 2012]
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UniProt Comments for CD16

Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
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Research Articles on CD16

1. In Taiwanese individuals, a low FCGR3A copy number is a common risk factor for systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA), while a low FCGR3B copy number confers a risk of SLE but not RA.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD16 antibody

 Reference Product  PubMed Publications
 SYK antibody  >4 publications with CD16 and SYK
 LILRB3 antibody  >1 publications with CD16 and LILRB3

Pathways associated with anti-CD16 antibody

 Products by Pathway  Pathway Diagram
 Leishmaniasis Pathway antibodies  Leishmaniasis Pathway Diagram
 Leishmaniasis Pathway antibodies  Leishmaniasis Pathway Diagram
 Natural Killer Cell Mediated Cytotoxicity Pathway antibodies  Natural Killer Cell Mediated Cytotoxicity Pathway Diagram
 Natural Killer Cell Mediated Cytotoxicity Pathway antibodies  Natural Killer Cell Mediated Cytotoxicity Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Staphylococcus Aureus Infection Pathway antibodies  Staphylococcus Aureus Infection Pathway Diagram
 Staphylococcus Aureus Infection Pathway antibodies  Staphylococcus Aureus Infection Pathway Diagram

Diseases associated with anti-CD16 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >69 publications with CD16 and Inflammation
 Cardiovascular Diseases Antibodies  >60 publications with CD16 and Cardiovascular Diseases
 Necrosis Antibodies  >57 publications with CD16 and Necrosis
 Syndrome Antibodies  >34 publications with CD16 and Syndrome
 Lupus Nephritis Antibodies  >17 publications with CD16 and Lupus Nephritis
 Sepsis Antibodies  >9 publications with CD16 and Sepsis
 Pain Antibodies  >8 publications with CD16 and Pain
 Agranulocytosis Antibodies  >7 publications with CD16 and Agranulocytosis
 Granulomatosis with Polyangiitis Antibodies  >7 publications with CD16 and Granulomatosis with Polyangiitis
 Pleural Effusion Antibodies  >3 publications with CD16 and Pleural Effusion

Organs/Tissues associated with anti-CD16 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >355 publications with CD16 and Blood
 Kidney Antibodies  >20 publications with CD16 and Kidney
 Lung Antibodies  >15 publications with CD16 and Lung
 Uterus Antibodies  >11 publications with CD16 and Uterus
 Liver Antibodies  >8 publications with CD16 and Liver
 Placenta Antibodies  >4 publications with CD16 and Placenta
 Brain Antibodies  >4 publications with CD16 and Brain
 Prostate Antibodies  >3 publications with CD16 and Prostate
 Pancreas Antibodies  >2 publications with CD16 and Pancreas
 Stomach Antibodies  >2 publications with CD16 and Stomach
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