Rat Anti-CD8 ALPHA Monoclonal Antibody – Cat# MBS214452

CD8 ALPHA Antibody – Rat Monoclonal

Name: CD8 ALPHA Antibody; Monoclonal; Rat; MyBioSource
Brand: MyBioSource
SKU: MBS214452
Price: 540 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS214452

Rat anti Mouse CD8 alpha antibody, clone 53-6.7 detects mouse CD8a, the alpha chain of the CD8 antigen. CD8 is expressed on T-cells, and exists either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. CD8… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

CD8 ALPHA, Monoclonal Antibody

Full Product Name

RAT ANTI MOUSE CD8 ALPHA

Product Gene Name

anti-CD8 ALPHA antibody

Clonality

Monoclonal

Isotype

IgG2a

Clone Number

53-6.7

Host

Rat

Form/Format

Purified
Purified IgG - liquid

Concentration

IgG concentration 0.5mg/ml (lot specific)

Preparation and Storage

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Molecular Weight

27,456 Da

3D Structure

ModBase 3D Structure for P01731

Perservative Stabilisers

0.09% Sodium Azide (NaN3)
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen

Mouse thymus or spleen.
Buffer Solution||Phosphate buffered saline

Target Species

Mouse

Applications Tested/Suitable for anti-CD8 ALPHA antibody

Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP)

Application Notes for anti-CD8 ALPHA antibody

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.
Immunohistology: This item has been reported as working well on Zinc-fixed paraffin-embedded sections. However, inconsistent results have been reported on formalin-fixed paraffin-embedded tissue sections.
Flow Cytometry: Minimum Dilution: 1/20

Other Notes

Small volumes of anti-CD8 ALPHA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD8 ALPHA purchase

MBS214452 is an antibody targeting CD8 ALPHA. Also known as RAT ANTI MOUSE CD8 ALPHA. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD8 ALPHA. It may not necessarily be applicable to this product.

NCBI GI #

124487437

NCBI GeneID

12525

NCBI Accession #

NP_001074579.1

UniProt Primary Accession #

P01731

UniProt Related Accession #

P01731

GenBank Nucleotide #

NM_001081110.2

NCBI Official Full Name

T-cell surface glycoprotein CD8 alpha chain isoform 1

NCBI Official Synonym Full Names

CD8 antigen, alpha chain

NCBI Official Symbol

Cd8a

NCBI Official Synonym Symbols

Ly-2; Ly-B; Ly-35; Lyt-2; BB154331

UniProt Protein Name

T-cell surface glycoprotein CD8 alpha chain

UniProt Synonym Protein Names

T-cell surface glycoprotein Lyt-2; CD_antigen: CD8a

Protein Family

T-cell surface glycoprotein

UniProt Gene Name

Cd8a

UniProt Synonym Gene Names

Lyt-2; Lyt2

UniProt Entry Name

CD8A_MOUSE

NCBI Protein Information

T-cell surface glycoprotein CD8 alpha chain; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell surface glycoprotein Lyt-2

UniProt Comments for CD8 ALPHA

CD8A: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; protein homodimerization activity; protein kinase binding

Biological Process: cell surface receptor linked signal transduction; T cell activation; immune system process; T cell mediated immunity; positive regulation of calcium-mediated signaling; cytotoxic T cell differentiation; defense response to virus

Research Articles on CD8 ALPHA

1. CD8alpha DCs, rather than CD8 T cells, are responsible for enhanced viral latency and recurrences.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD8 ALPHA antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Antigen Processing And Presentation Pathway antibodies	Antigen Processing And Presentation Pathway Diagram
Antigen Processing And Presentation Pathway antibodies	Antigen Processing And Presentation Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway antibodies	Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway Diagram
Primary Immunodeficiency Pathway antibodies	Primary Immunodeficiency Pathway Diagram

Diseases associated with anti-CD8 ALPHA antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>19 publications with CD8 ALPHA and Inflammation
Necrosis Antibodies	>12 publications with CD8 ALPHA and Necrosis
Cardiovascular Diseases Antibodies	>9 publications with CD8 ALPHA and Cardiovascular Diseases
Neoplasms, Experimental Antibodies	>8 publications with CD8 ALPHA and Neoplasms, Experimental
Carcinoma Antibodies	>7 publications with CD8 ALPHA and Carcinoma
Liver Diseases Antibodies	>7 publications with CD8 ALPHA and Liver Diseases
Kidney Diseases Antibodies	>5 publications with CD8 ALPHA and Kidney Diseases
Fibrosis Antibodies	>4 publications with CD8 ALPHA and Fibrosis
Liver Neoplasms Antibodies	>3 publications with CD8 ALPHA and Liver Neoplasms
Heart Diseases Antibodies	>3 publications with CD8 ALPHA and Heart Diseases

Organs/Tissues associated with anti-CD8 ALPHA antibody

Organ/Tissue Name	Pubmed Publications
Spleen Antibodies	>26 publications with CD8 ALPHA and Spleen
Thymus Antibodies	>15 publications with CD8 ALPHA and Thymus
Bone Marrow Antibodies	>12 publications with CD8 ALPHA and Bone Marrow
Thyroid Antibodies	>3 publications with CD8 ALPHA and Thyroid
Embryonic Tissue Antibodies	>2 publications with CD8 ALPHA and Embryonic Tissue