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CD8 BETA Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS214554

Rat anti Mouse CD8 beta antibody, clone YTS156.7.7 recognizes the murine T-cell surface glycoprotein CD8 beta chain, also known as CD8beta, LY-3 or Lyt-3. CD8beta is a 213 amino acid ~25kDa single pass type-1 transmembrane glycoprotein bearing a single… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CD8 BETA, Monoclonal Antibody
Full Product Name
RAT ANTI MOUSE CD8 BETA:RPE
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
YTS156.7.7
Host
Rat
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Concentration
IgG concentration 0.1mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch.
Molecular Weight
24,288 Da
3D Structure
ModBase 3D Structure for P10300
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation||Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Target Species
Mouse
Applications Tested/Suitable for anti-CD8 BETA antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD8 BETA antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/5

Other Notes

Small volumes of anti-CD8 BETA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD8 BETA purchase

MBS214554 is an antibody targeting CD8 BETA. Also known as RAT ANTI MOUSE CD8 BETA:RPE. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD8 BETA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
T-cell surface glycoprotein CD8 beta chain
NCBI Official Synonym Full Names
CD8 antigen, beta chain 1
NCBI Official Symbol
Cd8b1
NCBI Official Synonym Symbols
Cd8b; Ly-3; Ly-C; Lyt-3
UniProt Protein Name
T-cell surface glycoprotein CD8 beta chain
UniProt Synonym Protein Names
Lymphocyte antigen 3; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein Lyt-3; CD_antigen: CD8b
UniProt Gene Name
Cd8b
UniProt Synonym Gene Names
Cd8b1; Ly-3; Lyt-3; Lyt3
UniProt Entry Name
CD8B_MOUSE
NCBI Protein Information
T-cell surface glycoprotein CD8 beta chain; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein Lyt-3; lymphocyte antigen 3

UniProt Comments for CD8 BETA

CD8B: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding

Biological Process: immune system process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-CD8 BETA antibody

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