Mouse Anti-CA125 Monoclonal Antibody – Cat# MBS214597

CA125 Antibody – Mouse Monoclonal

Name: CA125 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS214597
Price: 605 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS214597

MBS214597 is an antibody targeting CA125. Also known as MOUSE ANTI HUMAN CA125. Reactive in Human. Host: Mouse. More details

$605 / 0.2 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CA125, Monoclonal Antibody

Full Product Name

MOUSE ANTI HUMAN CA125

Product Gene Name

anti-CA125 antibody

Product Synonym Names

MUCIN 16

Clonality

Monoclonal

Isotype

IgG1

Clone Number

X75

Host

Mouse

Species Reactivity

Human

Form/Format

Purified IgG - liquid

Purity/Purification

Purified IgG prepared by ion exchange chromatography

Concentration

IgG concentration 1.0mg/ml (lot specific)

Specificity

Mouse anti Human CA125 antibody, clone X75 recognizes the ovarian cancer mucin CA125 (also known as MUCIN 16). CA125 consists of a short cytoplasmic tail, a transmembrane domain and an exceptionally large glycosylated extracellular domain. The extracellular domain is dominated by a large number of 156-amino acid repeat units, over 60 in all. These repeats are known to bind the antibodies OC125 and M-11.
CA125 is present within normal ovarian, endometrium, endocervix and fallopian tissue, but levels are elevated in over 90% of women with advanced ovarian cancer. CA125 levels may also be elevated in other cancers and non-cancerous conditions such as peritonitis, endometriosis and liver cirrhosis. Levels may also fluctuate during the menstrual cycle and occasionally increases in pregnancy, making CA125 an unreliable marker for ovarian cancer in premenopausal women. The function of CA125 in healthy tissue is unknown but it is thought to provide a protective lubricating barrier against particles and infectious agents at mucosal surfaces.
CA125 was concluded by the International Society of Oncology and BioMarkers (ISOBM) to contain two major antigenic domains. Mouse anti Human CA125 antibody, clone X75 recognizes the epitope specificity group B, similar to the M-11 antibody (Nustad et al. 2002). Mouse anti Human CA125 antibody, clone X75 has been used successfully as both a capture and detection reagent in the development of sensitive ELISA assays for the measurement of CA125 antigen in human plasma samples.

Preparation and Storage

Store at +4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody.

OMIM

606154

3D Structure

ModBase 3D Structure for Q8WXI7

Target Species

Human

Immunogen

CA125 antigen purified from human ovarian carcinoma.

Buffer Solution

TRIS buffered saline

Preservative Stabilizers

0.09% Sodium Azide (NaN₃)

Fusion Partners

Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.

Applications Tested/Suitable for anti-CA125 antibody

ELISA, Western Blot (WB)

Application Notes for anti-CA125 antibody

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Other Notes

Small volumes of anti-CA125 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CA125. It may not necessarily be applicable to this product.

NCBI GI #

83367077

NCBI GeneID

94025

NCBI Accession #

NP_078966.2

UniProt Primary Accession #

Q8WXI7

UniProt Secondary Accession #

Q6ZQW5; Q96RK2

UniProt Related Accession #

Q8WXI7

GenBank Nucleotide #

NM_024690.2

NCBI Official Full Name

mucin-16

NCBI Official Synonym Full Names

mucin 16, cell surface associated

NCBI Official Symbol

MUC16

NCBI Official Synonym Symbols

CA125

UniProt Protein Name

Mucin-16

UniProt Synonym Protein Names

Ovarian cancer-related tumor marker CA125; CA-125; Ovarian carcinoma antigen CA125

Protein Family

Mucin

UniProt Gene Name

MUC16

UniProt Synonym Gene Names

CA125; MUC-16; CA-125

UniProt Entry Name

MUC16_HUMAN

NCBI Protein Information

mucin-16; CA125 ovarian cancer antigen; ovarian cancer-related tumor marker CA125; ovarian carcinoma antigen CA125

UniProt Comments for CA125

MUC16: Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.

Protein type: Cell adhesion; Membrane protein, peripheral; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: extracellular space; extrinsic to membrane; Golgi lumen; integral to membrane; plasma membrane; vesicle

Molecular Function: protein binding

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; cell adhesion; post-translational protein modification

Research Articles on CA125

1. High serum CA125 levels are associated with low response to therapy in ovarian cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CA125 antibody

Reference Product	PubMed Publications
MUC1 antibody	>83 publications with CA125 and MUC1
MUC4 antibody	>55 publications with CA125 and MUC4
MUC2 antibody	>19 publications with CA125 and MUC2
MUC7 antibody	>11 publications with CA125 and MUC7
MUC13 antibody	>10 publications with CA125 and MUC13
MUC20 antibody	>7 publications with CA125 and MUC20
MUC15 antibody	>7 publications with CA125 and MUC15
LGALS1 antibody	>3 publications with CA125 and LGALS1
MSLN antibody	>3 publications with CA125 and MSLN

Pathways associated with anti-CA125 antibody

Products by Pathway	Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
O-linked Glycosylation Pathway antibodies	O-linked Glycosylation Pathway Diagram
O-linked Glycosylation Of Mucins Pathway antibodies	O-linked Glycosylation Of Mucins Pathway Diagram
Post-translational Protein Modification Pathway antibodies	Post-translational Protein Modification Pathway Diagram
Termination Of O-glycan Biosynthesis Pathway antibodies	Termination Of O-glycan Biosynthesis Pathway Diagram

Diseases associated with anti-CA125 antibody

Disease Name	Pubmed Publications
Ovarian Neoplasms Antibodies	>211 publications with CA125 and Ovarian Neoplasms
Neoplasm Metastasis Antibodies	>28 publications with CA125 and Neoplasm Metastasis
Ascites Antibodies	>25 publications with CA125 and Ascites
Respiratory Tract Diseases Antibodies	>21 publications with CA125 and Respiratory Tract Diseases
Lung Neoplasms Antibodies	>19 publications with CA125 and Lung Neoplasms
Inflammation Antibodies	>16 publications with CA125 and Inflammation
Lung Diseases Antibodies	>14 publications with CA125 and Lung Diseases
Skin Diseases Antibodies	>13 publications with CA125 and Skin Diseases
Heart Diseases Antibodies	>11 publications with CA125 and Heart Diseases
Necrosis Antibodies	>10 publications with CA125 and Necrosis

Organs/Tissues associated with anti-CA125 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>221 publications with CA125 and Blood
Eye Antibodies	>56 publications with CA125 and Eye
Lung Antibodies	>33 publications with CA125 and Lung
Ovary Antibodies	>29 publications with CA125 and Ovary
Lymph Node Antibodies	>26 publications with CA125 and Lymph Node
Uterus Antibodies	>15 publications with CA125 and Uterus
Epididymis Antibodies	>14 publications with CA125 and Epididymis
Intestine Antibodies	>8 publications with CA125 and Intestine
Cervix Antibodies	>7 publications with CA125 and Cervix
Pancreas Antibodies	>7 publications with CA125 and Pancreas