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CD4 Antibody – Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS214751

Rat anti Dog CD4 antibody (YKIX302.9) is a monoclonal antibody specific for the canine CD4 cell surface antigen. Clone YKIX302.9 was clustered at the first Canine Leukocyte Antigen Workshop (CLAW) [Cobbold et al. 1992] along with clone CA13.1E4. Rat anti Dog… More details

$865 / 100 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD4, Monoclonal Antibody
Full Product Name
RAT ANTI DOG CD4:RPE
Product Gene Name
anti-CD4 antibody
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
YKIX302.9
Form/Format
RPE; Purified IgG conjugate ro R. Phycoerythrin (RPE)- lyophilized
Specificity
CD4
Preparation and Storage
Prior to reconstitution store at 4°C. Following reconstitution store at 4°C. Do not freeze. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommended microcentrifugation before use.
3D Structure
ModBase 3D Structure for P33705
Target Species
Dog
Reconstitution
Reconstitute with 1.0ml distilled
Max Ex/Em
Fluorophore: RPE 488nm laser; Excitation Max (nm): 496; Emission Max (nm): 578
Immunogen
Canine concanavilin A activated T cell blast.
Preparation
Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilizer
0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
Fusion Partners
Spleen cells from immunised DA rats were fused with cells of the Y3/Ag 1.2.3 rat myeloma cell line.
Applications Tested/Suitable for anti-CD4 antibody
Flow Cytometry (FC)
Application Notes for anti-CD4 antibody
FC: Neat-1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It isrecommended that the user titrates the antibody for use in their own system using appropriatenegative/positive controls

Flow Cytometry||Use 10ul of the suggested working dilution to label 10^6 cells or 100ul whole blood.

Testing Data of anti-CD4 antibody

Staining of canine peripheral blood lymphocytes with Rat anti Canine CD4: APC
Anti-CD4 Monoclonal Antibody – Cat# MBS214751

Testing Data of anti-CD4 antibody

Staining of canine peripheral blood lymphocytes with Rat anti Canine CD4: FITC (MBS214750)
Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS214751

Testing Data of anti-CD4 antibody

Published customer image: Survival rates of animals with canine mammary carcinoma. Kaplan-Meier survival curves for animals for All (MC-BMT and MC) animals categorized according to the relative percentage of CD4+ T-cells (33.3%) (A). Animals were submitted to quarterly follow-ups during twelve months and survival rates expressed in days between the surgical excisions of the end of follow-up as described in Methods. The survival curves were estimated with the Kaplan-Meier method followed by Log-rank test. Correlation analysis highlighted the significant association between the percentages of CD4+ T-cells and CD8+ T-cells with the animal survival in days (B).From: Estrela-Lima et al. BMC Cancer 2010 10:256.
Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS214751

Testing Data of anti-CD4 antibody

Published customer image: Immunophenotypic profile of tumor infiltrating lymphocyte in canine mammary carcinomas. Analysis of tumor infiltrating T-cells, B-lymphocytes and T-cell subsets from MC-BMT or MC (A), further subcategorized according to the absence (-) or presence (+) of lymph node metastasis (-) (B). Lymphocyte populations and subsets were identified by flow cytometric immunostaining as described in Material and Methods. Data were expressed as percentage of positive cells within gated lymphocytes and CD4+/CD8+ T-cell ratio. Significant differences at p < 0.05 are highlighted by asterisk.From: Estrela-Lima et al. BMC Cancer 2010 10:256.
Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS214751

Testing Data of anti-CD4 antibody

Published customer image: CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.
Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS214751

Testing Data of anti-CD4 antibody

Staining of canine peripheral blood lymphocytes with Rat anti Canine CD4: Alexa Fluor 488
Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS214751

Other Notes

Small volumes of anti-CD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD4 purchase

MBS214751 is an antibody targeting CD4. Also known as RAT ANTI DOG CD4:RPE.

Additional Details

NCBI/Uniprot data below describe general gene information for CD4. It may not necessarily be applicable to this product.

NCBI GI #
55742794
NCBI GeneID
403931
NCBI Accession #
NP_001003252.1
UniProt Primary Accession #
P33705
UniProt Related Accession #
P33705
GenBank Nucleotide #
NM_001003252.1
NCBI Official Full Name
T-cell surface glycoprotein CD4
NCBI Official Symbol
CD4
UniProt Protein Name
T-cell surface glycoprotein CD4
UniProt Synonym Protein Names
T-cell surface antigen T4/Leu-3; CD_antigen: CD4
Protein Family
T-cell surface glycoprotein
UniProt Gene Name
CD4
UniProt Entry Name
CD4_CANFA
NCBI Protein Information
T-cell surface glycoprotein CD4; CD4 antigen (p55); T-cell surface antigen T4/Leu-3

UniProt Comments for CD4

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD4 antibody

 Reference Product  PubMed Publications
 PTPRC antibody  >2890 publications with CD4 and PTPRC
 CXCR4 antibody  >2112 publications with CD4 and CXCR4
 LCK antibody  >797 publications with CD4 and LCK
 IFNG antibody  >160 publications with CD4 and IFNG
 HLA-DMA antibody  >56 publications with CD4 and HLA-DMA

Pathways associated with anti-CD4 antibody

 Products by Pathway  Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cytokines And Inflammatory Response Pathway antibodies  Cytokines And Inflammatory Response Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Primary Immunodeficiency Pathway antibodies  Primary Immunodeficiency Pathway Diagram
 Primary Immunodeficiency Pathway antibodies  Primary Immunodeficiency Pathway Diagram
 T Cell Receptor Signaling Pathway antibodies  T Cell Receptor Signaling Pathway Diagram

Diseases associated with anti-CD4 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >12218 publications with CD4 and Inflammation
 Disease Models, Animal Antibodies  >9067 publications with CD4 and Disease Models, Animal
 Necrosis Antibodies  >7648 publications with CD4 and Necrosis
 Lung Diseases Antibodies  >6768 publications with CD4 and Lung Diseases
 Liver Diseases Antibodies  >4684 publications with CD4 and Liver Diseases
 AIDS-Related Opportunistic Infections Antibodies  >4364 publications with CD4 and AIDS-Related Opportunistic Infections
 Carcinoma Antibodies  >3428 publications with CD4 and Carcinoma
 Cardiovascular Diseases Antibodies  >3187 publications with CD4 and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >2980 publications with CD4 and Neoplasms, Experimental
 Vascular Diseases Antibodies  >2496 publications with CD4 and Vascular Diseases

Organs/Tissues associated with anti-CD4 antibody

 Organ/Tissue Name  Pubmed Publications
 Uncharacterized Tissue Antibodies  >20 publications with CD4 and Uncharacterized Tissue
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