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Pi-3 KINASE P85 SUBUNIT ALPHA (PIK3R1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS214826

Mouse anti PI-3 Kinase p85 Subunit alpha antibody, clone U5 recognizes the bcr-homology domain of the p85 alpha subunit of bovine PI 3 kinase. More details

$605 / 0.2 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PI-3 KINASE p85 SUBUNIT ALPHA (PIK3R1), Monoclonal Antibody
Full Product Name
MOUSE ANTI PI-3 KINASE p85 SUBUNIT ALPHA
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1
Clone Number
U5
Host
Mouse
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
83,497 Da
Perservative Stabilisers
0.09% Sodium Azide
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Baculovirus expressed (Recombinant) bovine p85 alpha subunit of PI-3 kinase.
Fusion Partners||Spleen cells from immunised mice were fused with cells of the NS1 mouse myeloma cell line.
Buffer Solution||Phosphate buffered saline
Target Species
Bovine
Applications Tested/Suitable for anti-PIK3R1 antibody
Immunohistology Frozen, Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-PIK3R1 antibody
Western Blotting: Minimum Dilution: 1/100; Maximum Dilution: 1/1000

Testing Data of anti-PIK3R1 antibody

Jurkat Whole Cell lysate probed with Mouse anti PI-3 Kinase p85 Alpha subunit
Mouse Anti-PIK3R1 Monoclonal Antibody – Cat# MBS214826

Other Notes

Small volumes of anti-PIK3R1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PIK3R1 purchase

MBS214826 is an antibody targeting PIK3R1. Also known as MOUSE ANTI PI-3 KINASE p85 SUBUNIT ALPHA. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PIK3R1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
phosphatidylinositol 3-kinase regulatory subunit alpha
NCBI Official Symbol
PIK3R1
UniProt Protein Name
Phosphatidylinositol 3-kinase regulatory subunit alpha
UniProt Synonym Protein Names
Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha
UniProt Gene Name
PIK3R1
UniProt Synonym Gene Names
PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha
UniProt Entry Name
P85A_BOVIN
NCBI Protein Information
phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase p85 subunit alpha; PI3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphoinositide-3-kinase, regulatory subunit 1 (p85 alpha); phosphoinositide-3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); ptdIns-3-kinase p85-alpha; ptdIns-3-kinase regulatory subunit alpha; ptdIns-3-kinase regulatory subunit p85-alpha

UniProt Comments for PIK3R1

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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