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Semaphorin 5a (SEMA5A) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2151609
SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

MBS2151609 is an antibody targeting SEMA5A. Also known as Monoclonal Antibody to Semaphorin 5A (SEMA5A). Reactive in Human, Rat. Host: Mouse. More details

$230 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Semaphorin 5A (SEMA5A), Monoclonal Antibody
Full Product Name
Monoclonal Antibody to Semaphorin 5A (SEMA5A)
Product Gene Name
Product Synonym Names
Semaphorin 5A; SEMAF; SemF; Semaphorin-F; Sema Domain,Immunogloulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 5A
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
C3
Host
Mouse
Species Reactivity
Human, Rat
Form/Format
Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Purity/Purification
Protein A + Protein G affinity chromatography
Concentration
1mg/mL (lot specific)
Specificity
The antibody is a mouse monoclonal antibody raised against SEMA5A. It has been selected for its ability to recognize SEMA5A in immunohistochemical staining and western blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Store at -20 degree C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Matching Pairs
Unconjugated Antibody: SEMA5A Clone #C3 (MBS2151609)
HRP Conjugated Antibody: SEMA5A Clone #C3 (MBS2140549)
Molecular Weight
120,615 Da
Immunogen Sequence Positions
Val319~Gln668
3D Structure
ModBase 3D Structure for Q13591
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant Semaphorin 5A (SEMA5A)
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-SEMA5A antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes for anti-SEMA5A antibody
WB: 0.5-2ug/mL
IHC: 5-20ug/mL
ICC: 5-20ug/mL

Western Blot (WB) of anti-SEMA5A antibody

Western Blot; Sample: Rat CerebuM lysate Primary Ab: 0.2ug/ml Mouse Anti-human SEMA5A Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (#MBS2086035)
Mouse Anti-SEMA5A Monoclonal Antibody – Cat# MBS2151609

Other Notes

Small volumes of anti-SEMA5A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SEMA5A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
semaphorin-5A
NCBI Official Synonym Full Names
semaphorin 5A
NCBI Official Symbol
SEMA5A
NCBI Official Synonym Symbols
semF; SEMAF
UniProt Protein Name
Semaphorin-5A
UniProt Synonym Protein Names
Semaphorin-F; Sema F
Protein Family
UniProt Gene Name
SEMA5A
UniProt Synonym Gene Names
SEMAF; Sema F
NCBI Protein Information
semaphorin-5A

NCBI Summary for SEMA5A

This gene belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.[provided by RefSeq, Jan 2010]
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UniProt Comments for SEMA5A

Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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