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Nerve Growth Factor (NGF) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2151918
SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

MBS2151918 is an antibody targeting NGF. Also known as Monoclonal Antibody to Nerve Growth Factor (NGF). Reactive in Human, Rat. Host: Mouse. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Nerve Growth Factor (NGF), Monoclonal Antibody
Full Product Name
Monoclonal Antibody to Nerve Growth Factor (NGF)
Product Gene Name
Product Synonym Names
Nerve Growth Factor; NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
C2
Host
Mouse
Species Reactivity
Human, Rat
Form/Format
Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
1mg/mL (lot specific)
Specificity
The antibody is a mouse monoclonal antibody raised against NGF. It has been selected for its ability to recognize NGF in immunohistochemical staining and western blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Store at -20 degree C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Matching Pairs
Unconjugated Antibody: NGF Clone #C2 (MBS2151918)
HRP Conjugated Antibody: NGF Clone #C2 (MBS2140860)
Molecular Weight
27,010 Da
Immunogen Sequence Positions
Glu19~Gly241
3D Structure
ModBase 3D Structure for P25427
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant Nerve Growth Factor (NGF)
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-NGF antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes for anti-NGF antibody
WB: 0.5-2ug/mL
IHC: 5-20ug/mL
ICC: 5-20ug/mL

Immunohistochemistry (IHC) of anti-NGF antibody

DAB staining on IHC-P; Sample: Rat CerebuM Tissue; Primary Ab: 20ug/ml Mouse Anti-Rat NGF Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (#MBS2086035)
Mouse Anti-NGF Monoclonal Antibody – Cat# MBS2151918

Immunohistochemistry (IHC) of anti-NGF antibody

DAB staining on IHC-P;Sample: Rat CerebuM TissuePrimary Ab: 20ug/ml Mouse Anti-Rat NGF AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody(#MBS2086035)
Mouse Anti-NGF Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2151918

Western Blot (WB) of anti-NGF antibody

Western Blot; Sample: Hela cell lysate Primary Ab: 2ug/ml Mouse Anti-Rat NGF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (#MBS2086035)
Mouse Anti-NGF Monoclonal Antibody, Western Blot (WB) – Cat# MBS2151918

Other Notes

Small volumes of anti-NGF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NGF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
Beta-nerve growth factor
NCBI Official Synonym Full Names
nerve growth factor (beta polypeptide)
NCBI Official Symbol
Ngf
NCBI Official Synonym Symbols
Ngfb; beta-NGF
UniProt Protein Name
Beta-nerve growth factor
UniProt Gene Name
Ngf
UniProt Synonym Gene Names
Ngfb; Beta-NGF
UniProt Entry Name
NGF_RAT
NCBI Protein Information
beta-nerve growth factor

UniProt Comments for NGF

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-NGF antibody

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