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CD3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS215210

Mouse anti Dog CD3 antibody, clone CA17.2A12 recognises the canine CD3 cell surface antigen, expressed by thymocytes and mature T lymphocytes. CD3 is engaged in the surface expression of the T-cell antigen receptor (TCR) and the signal transduction pathway… More details

$770 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD3, Monoclonal Antibody
Full Product Name
MOUSE ANTI DOG CD3:FITC
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1
Clone Number
CA17.2A12
Host
Mouse
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
22,642 Da
3D Structure
ModBase 3D Structure for P27597
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation||Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Target Species
Dog
Applications Tested/Suitable for anti-CD3 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD3 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. N.B.. This item should not be used with mouse anti canine B-cells, in dual colour flow cytometry, due to non-specific interactions between the two reagents.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10

Testing Data of anti-CD3 antibody

Published customer image: CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.
Mouse Anti-CD3 Monoclonal Antibody – Cat# MBS215210

Testing Data of anti-CD3 antibody

Staining of canine peripheral blood lymphocytes with Mouse anti Canine CD3 (MBS215211)
Mouse Anti-CD3 Monoclonal Antibody, Testing Data – Cat# MBS215210

Other Notes

Small volumes of anti-CD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD3 purchase

MBS215210 is an antibody targeting CD3. Also known as MOUSE ANTI DOG CD3:FITC. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
T-cell surface glycoprotein CD3 epsilon chain
NCBI Official Symbol
CD3E
UniProt Protein Name
T-cell surface glycoprotein CD3 epsilon chain
UniProt Synonym Protein Names
CD_antigen: CD3e
Protein Family
UniProt Gene Name
CD3E
UniProt Entry Name
CD3E_CANFA
NCBI Protein Information
T-cell surface glycoprotein CD3 epsilon chain; CD3E antigen, epsilon polypeptide (TiT3 complex)

UniProt Comments for CD3

The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. Required for normal immune responses.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD3 antibody

Organs/Tissues associated with anti-CD3 antibody

 Organ/Tissue Name  Pubmed Publications
 Female Genital Antibodies  >2 publications with CD3 and Female Genital
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