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Desmoglein 3 (DSG3) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2152197
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MBS2152197 is an antibody targeting DSG3. Also known as Monoclonal Antibody to Desmoglein 3 (DSG3). Reactive in Human. Host: Mouse. More details

$210 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Desmoglein 3 (DSG3), Monoclonal Antibody
Full Product Name
Monoclonal Antibody to Desmoglein 3 (DSG3)
Product Gene Name
Product Synonym Names
Desmoglein 3; CDHF6; PVA; 130 kDa pemphigus ulgaris antigen; Cadherin family member 6
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
C17
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Purity/Purification
Protein A + Protein G affinity chromatography
Concentration
1mg/mL (lot specific)
Specificity
The antibody is a mouse monoclonal antibody raised against DSG3. It has been selected for its ability to recognize DSG3 in immunohistochemical staining and western blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Store at -20 degree C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Matching Pairs
Unconjugated Antibody: DSG3 Clone #C17 (MBS2152197)
HRP Conjugated Antibody: DSG3 Clone #C17 (MBS2141140)
Molecular Weight
107,533 Da
Immunogen Sequence Positions
Ala334~Pro604
OMIM
169615
3D Structure
ModBase 3D Structure for P32926
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant Desmoglein 3 (DSG3)
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-DSG3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes for anti-DSG3 antibody
WB: 0.5-2ug/mL
IHC: 5-20ug/mL
ICC: 5-20ug/mL

Western Blot (WB) of anti-DSG3 antibody

Western Blot; Sample: Hela cell lysate Primary Ab: 1.5 ug/ml Mouse Anti-human DSG3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (#MBS2086035)
Mouse Anti-DSG3 Monoclonal Antibody – Cat# MBS2152197

Other Notes

Small volumes of anti-DSG3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DSG3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Desmoglein-3
NCBI Official Synonym Full Names
desmoglein 3
NCBI Official Symbol
DSG3
NCBI Official Synonym Symbols
PVA; CDHF6
UniProt Protein Name
Desmoglein-3
UniProt Synonym Protein Names
130 kDa pemphigus vulgaris antigen; PVA; Cadherin family member 6
Protein Family
UniProt Gene Name
DSG3
UniProt Synonym Gene Names
CDHF6; PVA
UniProt Entry Name
DSG3_HUMAN
NCBI Protein Information
desmoglein-3; cadherin family member 6; pemphigus vulgaris antigen; 130-kD pemphigus vulgaris antigen; 130 kDa pemphigus vulgaris antigen

NCBI Summary for DSG3

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris. [provided by RefSeq, Jul 2008]
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UniProt Comments for DSG3

DSG3: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 18q12.1

Cellular Component: desmosome; plasma membrane; integral to membrane; cytosol

Molecular Function: calcium ion binding

Biological Process: apoptosis; homophilic cell adhesion; cell structure disassembly during apoptosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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