Mouse Anti-AST Monoclonal Antibody – Cat# MBS2152449

Western Blot; Sample: Lane1: Rat Heart lysate; Lane2: Rat Liver lysate; Lane3: Rat Kidney lysate Primary Ab: 0.4ug/ml Mouse Anti-Rat AST Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (#MBS2086035)

Aspartate Aminotransferase (AST) Antibody – Mouse Monoclonal

Name: AST Antibody; Monoclonal; Mouse; Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS2152449
Price: 225 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2152449

MBS2152449 is an antibody targeting AST. Also known as Monoclonal Antibody to Aspartate Aminotransferase (AST). Reactive in Rat. Host: Mouse. More details

$225 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Aspartate Aminotransferase (AST), Monoclonal Antibody

Full Product Name

Monoclonal Antibody to Aspartate Aminotransferase (AST)

Product Gene Name

anti-AST antibody

Product Synonym Names

Aspartate Aminotransferase; cCAT; AAT; ASAT; SGOT; GOT1; Cysteine transaminase, cytoplasmic; Transaminase A; Aspartate Transaminase 1,Cytoplasmic; Glutamic-Oxaloacetic Transaminase 1,Soluble

Clonality

Monoclonal

Isotype

IgG1 Kappa

Clone Number

Host

Mouse

Species Reactivity

Rat

Form/Format

Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Purity/Purification

Protein A + Protein G affinity chromatography

Concentration

1mg/mL (lot specific)

Specificity

The antibody is a mouse monoclonal antibody raised against AST. It has been selected for its ability to recognize AST in immunohistochemical staining and western blotting.

Preparation and Storage

Store at 4 degree C for frequent use. Store at -20 degree C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Matching Pairs

Antibody: AST Clone #C6 (MBS2152449)
Immunogen: AST (MBS2009670)

Matching Pairs

Unconjugated Antibody: AST Clone #C6 (MBS2152449)
HRP Conjugated Antibody: AST Clone #C6 (MBS2141392)

Molecular Weight

46,429 Da

Immunogen Sequence Positions

Met1~Gln413

3D Structure

ModBase 3D Structure for P13221

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Immunogen

Recombinant Aspartate Aminotransferase (AST)

Product Categories/Family for anti-AST antibody

Monoclonal Antibodies (Primary Abs); Monoclonal antibodies; Enzyme & Kinase

ISO Certification

Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Applications Tested/Suitable for anti-AST antibody

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Application Notes for anti-AST antibody

WB: 0.5-2ug/mL
IHC: 5-20ug/mL
ICC: 5-20ug/mL

Other Notes

Small volumes of anti-AST antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AST. It may not necessarily be applicable to this product.

NCBI GI #

401461792

NCBI GeneID

24401

NCBI Accession #

NP_036703.2

UniProt Primary Accession #

P13221

UniProt Secondary Accession #

Q64570; Q6P721

UniProt Related Accession #

P13221

GenBank Nucleotide #

NM_012571.2

NCBI Official Full Name

aspartate aminotransferase, cytoplasmic

NCBI Official Synonym Full Names

glutamic-oxaloacetic transaminase 1

NCBI Official Symbol

Got1

NCBI Official Synonym Symbols

ASAT; cCAT; Aspat; Gaspat; cAspAT

UniProt Protein Name

Aspartate aminotransferase, cytoplasmic

UniProt Synonym Protein Names

Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A

Protein Family

Allatostatin

UniProt Gene Name

Got1

UniProt Synonym Gene Names

cAspAT; cCAT

NCBI Protein Information

aspartate aminotransferase, cytoplasmic

NCBI Summary for AST

cytosolic aspartate aminotransferase isozyme; enzyme activity in liver and kidney increase in response to glucocorticoids [RGD, Feb 2006]

UniProt Comments for AST

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.

Research Articles on AST

1. Hydrogen sulfide (H2 S) is synthesized in peripheral neurons through two mitochondrial enzymes, mercaptopyruvate sulfurtransferase (MPST)and cysteine aminotransferase (CAT).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AST antibody

Reference Product	PubMed Publications
Mdh1 antibody	>5 publications with AST and Mdh1
Ldhb antibody	>3 publications with AST and Ldhb
Mdh2 antibody	>2 publications with AST and Mdh2

Pathways associated with anti-AST antibody

Products by Pathway	Pathway Diagram
2-Oxocarboxylic Acid Metabolism Pathway antibodies	2-Oxocarboxylic Acid Metabolism Pathway Diagram
2-Oxocarboxylic Acid Metabolism Pathway antibodies	2-Oxocarboxylic Acid Metabolism Pathway Diagram
Alanine And Aspartate Metabolism Pathway antibodies	Alanine And Aspartate Metabolism Pathway Diagram
Alanine, Aspartate And Glutamate Metabolism Pathway antibodies	Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
Alanine, Aspartate And Glutamate Metabolism Pathway antibodies	Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies	Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Arginine Biosynthesis Pathway antibodies	Arginine Biosynthesis Pathway Diagram
Arginine Biosynthesis Pathway antibodies	Arginine Biosynthesis Pathway Diagram

Diseases associated with anti-AST antibody

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>6 publications with AST and Disease Models, Animal
Nervous System Diseases Antibodies	>6 publications with AST and Nervous System Diseases
Lung Neoplasms Antibodies	>6 publications with AST and Lung Neoplasms
Liver Diseases Antibodies	>5 publications with AST and Liver Diseases
Fatty Liver Antibodies	>3 publications with AST and Fatty Liver
Congenital Abnormalities Antibodies	>3 publications with AST and Congenital Abnormalities
Cardiovascular Diseases Antibodies	>3 publications with AST and Cardiovascular Diseases
Liver Neoplasms Antibodies	>2 publications with AST and Liver Neoplasms
Carcinoma, Hepatocellular Antibodies	>2 publications with AST and Carcinoma, Hepatocellular
Inflammation Antibodies	>2 publications with AST and Inflammation

Organs/Tissues associated with anti-AST antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>9 publications with AST and Liver
Lung Antibodies	>7 publications with AST and Lung
Brain Antibodies	>6 publications with AST and Brain
Small Intestine Antibodies	>4 publications with AST and Small Intestine
Muscle Antibodies	>4 publications with AST and Muscle
Heart Antibodies	>4 publications with AST and Heart
Spinal Cord Antibodies	>3 publications with AST and Spinal Cord
Artery Antibodies	>2 publications with AST and Artery
Olfactory Mucosa Antibodies	>1 publications with AST and Olfactory Mucosa
Adrenal Gland Antibodies	>1 publications with AST and Adrenal Gland