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CD16 Antibody – Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS215422

Mouse anti Pig CD16, clone G7 recognizes porcine CD16 also known as Fc-gamma RIII or the low affinity IgG (Fc) receptor III. Clone G7 was clustered as CD16 at the Second International Workshop to Define Swine Cluster of Differentiation (CD) Antigens… More details

$780 / 100 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD16, Monoclonal Antibody
Full Product Name
MOUSE ANTI PIG CD16:RPE
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1
Clone Number
G7
Form/Format
RPE; Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Specificity
CD16
Mouse anti Pig CD16, clone G7 recognizes porcine CD16 also known as Fc-gamma RIII or thelow affinity IgG (Fc) receptor III. Clone G7 was clustered as CD16 at the Second International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Saalmuller et al. 1998).

Mouse anti pig CD16 immunoprecipitates a protein of ~40 kDa from porcine neutrophils and NKcells (Wierda et al. 1993). Subsequent cloning and characterization of the G7 molecule indicatedthat G7 was the porcine homologue of Human CD16 (Halloran et al. 1994).
Preparation and Storage
Store at 4°C
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use
3D Structure
ModBase 3D Structure for Q28942
Target Species
Pig
Reconstitution
Reconstitute with 1.0 ml distilled water
Max Ex/Em
Fluorophore: RPE 488nm laser; Excitation Max (nm): 496; Emission Max (nm): 578
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
Immunogen
Porcine peripheral blood leucocytes
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3-X63-Ag8.653myeloma cell line
Applications Tested/Suitable for anti-CD16 antibody
Flow Cytometry (FC)
Application Notes for anti-CD16 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Testing Data of anti-CD16 antibody

Staining of porcine peripheral blood granulocytes with Mouse anti Porcine CD16:RPE
Anti-CD16 Monoclonal Antibody – Cat# MBS215422

Testing Data of anti-CD16 antibody

Staining of porcine peripheral blood granulocytes with Mouse anti Porcine CD16 (MBS212426GA) followed by Goat anti Mouse IgG:FITC (MBS235142)
Anti-CD16 Monoclonal Antibody, Testing Data – Cat# MBS215422

Other Notes

Small volumes of anti-CD16 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD16 purchase

MBS215422 is an antibody targeting CD16. Also known as MOUSE ANTI PIG CD16:RPE.

Additional Details

NCBI/Uniprot data below describe general gene information for CD16. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Low affinity immunoglobulin gamma Fc region receptor III
NCBI Official Symbol
FCGR3B
NCBI Official Synonym Symbols
FCGR3
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor III
UniProt Synonym Protein Names
Cytolytic trigger molecule G7; Fc-gamma RIII; FcRIII; CD_antigen: CD16
Protein Family
UniProt Gene Name
FCGR3
UniProt Synonym Gene Names
IgG Fc receptor III; FcRIII
UniProt Entry Name
FCGR3_PIG
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor III; FcgammaRIII a.1; cytolytic trigger molecule G7; fc-gamma RIII; fcRIII; igG Fc receptor III

UniProt Comments for CD16

Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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