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IFN GAMMA (IFNG) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS215513

Mouse anti Bovine interferon gamma antibody, clone CC330 recognizes bovine interferon gamma, a 143 amino acid ~20kDa immunomodulatory cytokine secreted predominantly by T lymphocytes in response to infection by invading miro-organisms. It enhances cytotoxic… More details

$845 / 0.5 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IFN GAMMA (IFNG), Monoclonal Antibody
Full Product Name
MOUSE ANTI BOVINE INTERFERON GAMMA
Product Gene Name
anti-IFNG antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
CC330
Host
Mouse
Species Reactivity
Sheep
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
19,393 Da
3D Structure
ModBase 3D Structure for P07353
Perservative Stabilisers
0.09% Sodium Azide
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Recombinant bovine IFN-gamma
Buffer Solution||Phosphate buffered saline
Target Species
Bovine
Applications Tested/Suitable for anti-IFNG antibody
ELISA (EIA), ELISpot (ES), Flow cytometry (FC/FACS)*
Application Notes for anti-IFNG antibody
ELISA: This clone may be used in a sandwich ELISA in combination with clone CC302 and recombinant bovine IFN gamma.
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
ELISA: Maximum Dilution: 5ug/ml as capture antibody
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.

Testing Data of anti-IFNG antibody

Published customer image: Bovine gamma delta T cells impair B. abortus replication in autologous macrophages via IFN- gamma . A. -F. Bovine macrophages were infected with B. abortus (30 bacteria:1 macrophage) and then fresh media or media containing autologous gamma delta T cells were added to infected macrophages. A., C., E. Macrophage colonization (triplicate wells/treatment) was monitored over time. *P
Mouse Anti-IFNG Monoclonal Antibody – Cat# MBS215513

Testing Data of anti-IFNG antibody

Published customer image: IFN-gamma response (log10 scale) in mesenteric lymph nodes, Peyer's Patches, popliteal LN and PBMC against GRA7, TLA and MIC3 in function of time.From: Verhelst D, De Craeye S, Entrican G, Dorny P, Cox E. Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep. BMC Vet Res. 2014 Dec 16;10(1):293.
Mouse Anti-IFNG Monoclonal Antibody, Testing Data – Cat# MBS215513

Testing Data of anti-IFNG antibody

Published customer image: gamma delta T cells require TNF-alpha to protect against B. abortus infection. C57BL/6 mice treated with anti-TCR gamma delta mAb or hamster IgG on day -1 and day 3 post-infection with 5x104 CFUs of B. abortus 2308. Some mice were also neutralized of their TNF-alpha on days -1 and 3. Seven days after infection, A. splenic weights and B. extent of brucellae colonization were determined. The mean +/- SEM of 10 mice/group is depicted; *P
Mouse Anti-IFNG Monoclonal Antibody, Testing Data – Cat# MBS215513

Testing Data of anti-IFNG antibody

Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P
Mouse Anti-IFNG Monoclonal Antibody, Testing Data – Cat# MBS215513

Other Notes

Small volumes of anti-IFNG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IFNG purchase

MBS215513 is an antibody targeting IFNG. Also known as MOUSE ANTI BOVINE INTERFERON GAMMA. Reactive in Sheep. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for IFNG. It may not necessarily be applicable to this product.

NCBI GI #
31982949
NCBI GeneID
281237
NCBI Accession #
NP_776511.1
UniProt Primary Accession #
P07353
UniProt Secondary Accession #
A7L687; A9QWQ4
UniProt Related Accession #
P07353
GenBank Nucleotide #
NM_174086.1
NCBI Official Full Name
interferon gamma
NCBI Official Synonym Full Names
interferon, gamma
NCBI Official Symbol
IFNG
UniProt Protein Name
Interferon gamma
Protein Family
Interferon
UniProt Gene Name
IFNG
UniProt Synonym Gene Names
BoIFNG; IFN-gamma
UniProt Entry Name
IFNG_BOVIN
NCBI Protein Information
interferon gamma; IFN-gamma; boIFNG; interferon gamma type 2; interferon-gamma

UniProt Comments for IFNG

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
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Research Articles on IFNG

1. These findings indicate that IFN-gamma production correlates negatively and the production of antibodies against N. caninum is uncorrelated with plasma pregnancy-associated glycoproteins levels.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-IFNG antibody

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 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram

Diseases associated with anti-IFNG antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >155 publications with IFNG and Necrosis
 Autoimmune Diseases Antibodies  >90 publications with IFNG and Autoimmune Diseases
 Respiratory Tract Diseases Antibodies  >71 publications with IFNG and Respiratory Tract Diseases
 Hepatitis Antibodies  >34 publications with IFNG and Hepatitis
 Lymphoproliferative Disorders Antibodies  >21 publications with IFNG and Lymphoproliferative Disorders
 Diabetes Mellitus, Type 1 Antibodies  >17 publications with IFNG and Diabetes Mellitus, Type 1
 HIV Infections Antibodies  >17 publications with IFNG and HIV Infections
 Hepatitis C Antibodies  >16 publications with IFNG and Hepatitis C
 Arthritis, Rheumatoid Antibodies  >15 publications with IFNG and Arthritis, Rheumatoid
 Leishmaniasis Antibodies  >11 publications with IFNG and Leishmaniasis

Organs/Tissues associated with anti-IFNG antibody

 Organ/Tissue Name  Pubmed Publications
 Mixed Antibodies  >12 publications with IFNG and Mixed
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