Published customer image: MLN and spleen cell IL-12 responses to R-848 stimulation as a function of age. MLN and spleen cells from 6- to 14-day-old neonates (closed circles), 20-day-old lambs (closed diamonds) and adults (open squares) were cultured in vitro for 48 h, with or without 0.5 ug/ml R-848. At the end of the culture period, supernatants were harvested and ELISA carried out to assess IL-12 secretion by MLN cells (A) or spleen cells (B). Medians are shown for each stimulus. Non-parametric Mann-Whitney tests were used to compare data for neonates, 20-day-old lambs and adults: *p=0.01; **p=0.005; ***p=0.001; ****p=0.0005.From: Ferret-Bernard S, Remot A, Lacroix-Lamand© S, Metton C, Bernardet N, et al. (2010) Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5(10): e13705.

Published customer image: Comparative IL-12 and IFN&gamma responses of neonatal and adult MLN cells following TLR agonist stimulation. Neonatal (closed circles) and adult (open squares) MLN cells were cultured in vitro with or without 12.5 ug/ml polyI:C, 0.5 ug/ml R-848 or 5 ug/ml Gardiquimod. Supernatants were harvested after 48 h of culture and ELISA was carried out for IL-12 (A) and IFN? (B) secretion. Non-specific cell stimulation was also carried out with 50 ng/ml PMA combined with 500 ng/ml ionomycin, with the supernatants assayed for IFN? detection 48 h later (C). Each circle or square represents one neonate or one adult, respectively. Medians are shown for each stimulus. Non-parametric Mann-Whitney tests were used to compare data for neonates and adults: *p=0.01; **p=0.005; ***p=0.001; ****p=0.0005.From: Ferret-Bernard S, Remot A, Lacroix-Lamand© S, Metton C, Bernardet N, et al. (2010) Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5(10): e13705.

Published customer image: IFN-gamma response (log10 scale) in mesenteric lymph nodes, Peyer's Patches, popliteal LN and PBMC against GRA7, TLA and MIC3 in function of time.From: Verhelst D, De Craeye S, Entrican G, Dorny P, Cox E. Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep. BMC Vet Res. 2014 Dec 16;10(1):293.

Published customer image: Roles of TGFbeta1 and IL-10 in regulating IL-12 responses to R-848. MLN cells from neonates (closed circles) and adults (open squares) were cultured in vitro for 48 h, with or without 0.5 ug/ml R-848, in the presence of rhTGFbeta1 (n = 7) (A) or rovIL-10 (n = 5) (B). Supernatants were harvested and ELISA was carried out to assess IL-12 secretion. The mean +/- SEM level of IL-12 secretion is shown (A, B). RNA was extracted and purified from freshly isolated MLN cells. IL-10 mRNA levels were determined by quantitative RT-PCR. Median normalised values are presented for neonates (closed circles) and adults (open squares) (C). MLN cells from neonates (closed circles) and adults (open squares) were stimulated in vitro for 48 h with or without 0.5 ug/ml R-848. Supernatants were harvested and ELISA carried out to assess IL-10 secretion. Medians are indicated. Non-parametric Mann-Whitney tests were used to compare data for neonates and adults: **p=0.001 (D). RNA was extracted and purified from freshly isolated MLN cells. Foxp3 mRNA levels were determined by quantitative RT-PCR. Median normalised values are presented for neonates (closed circles) and adults (open squares) (E). Comparison of the IL-12 response of neonate MLN cells to R-848 stimulation in culture medium supplemented with 10%FCS, 10% neonate autologous plasma or 10% adult plasma. Paired t-test between neonate and adult plasma were non-significant (F).From: Ferret-Bernard S, Remot A, Lacroix-Lamand© S, Metton C, Bernardet N, et al. (2010) Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5(10): e13705.