Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 (MBS215657) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). Medium power

Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 (MBS215657), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. High power

Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 488 (MBS215657A488)

Staining of J774 cell line with Rat anti Mouse CD206 following permeabilisation with Leucoperm

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 (MBS215657) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). Low power

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 (MBS215657) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). High power

Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 (MBS215657), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. Low power

Published customer image: Western blot analysis to determine type of activation of adult primary microglial cells produced from wild-type and IL-1 KO mice stimulated with IL-4, IL-13 or IL-4/IL-13 with or without IL-1beta. (A) Representative western blotting data of primary microglial cells produced from wild-type (wild) and IL-1 KO mice and exposed for 24 hours to IL-4, IL-13 or IL-4 plus IL-13 (IL-4/IL-13) with or without IL-1beta. Each lane expected to CD206 blotting were applied 7 ug of reduced samples. Non-reduced samples (5 ug) were applied to detect CD206. Densitometric analysis of COX2 (B), Ym1 (C), Arg-1 (D) and CD206 (E) (n = 3 each group). (B) COX2 levels are increased by exposure of cells to IL-1beta and are not influenced by IL-4 or IL-13 alone. The COX2 level was slightly enhanced by IL-1beta and IL-4 co-treatment. (C) Ym1 levels are increased by exposure of cells to IL-4 and IL-4/Il-13 and are synergistically increased by co-treatment with IL-1beta. However, only a low level of Ym1 is seen upon exposure of cells to IL-13, and is significantly less than that seen in response to exposure of cells to IL-4. (D) Arg-1 shows similar levels in response to exposure to IL-4 and IL-4/IL-13; these are synergistically increased by co-treatment with IL-1beta. However, low levels of Arg-1 are seen for exposure of cells to IL-13. (G) CD206 was detected in response to exposure of cells to both IL-4 and IL-4/IL-13 with or without IL-1beta; however, CD206 levels in IL-13-exposed samples were lower than those seen with the other treatments. Data are expressed as mean +/- SD (n = 3). *: P < 0.05, **: P < 0.01, ***: P < 0.001 compared with the IL-4-treated group without IL-1beta for each genotype (one-way ANOVA followed by Dunnett post-hoc test). ANOVA, analysis of variance; arg-1, arginase 1; COX 2, cyclooxygenase 2.From: Sato A, Ohtaki H, Tsumuraya T, Song D, Ohara K, Asano M, Iwakura Y, Atsumi T, Shioda S. Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury. J Neuroinflammation. 2012 Apr 7;9:65.

Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p