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CD3 Antibody – Hamster Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS216295

Hamster anti Mouse CD3 antibody, clone 145-2C11 detects CD3 epsilon (CD3e), a 20kDa transmembrane protein also known as CD3 or T3. CD3e is a member of the CD3 complex which consists of four subunits, gamma, delta, epsilon and zeta, and these are associated to… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD3, Monoclonal Antibody
Full Product Name
HAMSTER ANTI MOUSE CD3
Product Gene Name
anti-CD3 antibody
Clonality
Monoclonal
Isotype
IgG
Clone Number
145-2C11
Host
Hamster
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 0.5 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
21,393 Da
3D Structure
ModBase 3D Structure for P22646
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
H-2Kb - specific mouse cytotoxic T lymphocyte clone BM10-37.
Buffer Solution||Phosphate buffered saline
Target Species
Mouse
Applications Tested/Suitable for anti-CD3 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-CD3 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/200

Testing Data of anti-CD3 antibody

Mouse splenocytes stained with Hamster anti Mouse CD3:AlexaFluor488 (MBS214442A488)
Hamster Anti-CD3 Monoclonal Antibody – Cat# MBS216295

Testing Data of anti-CD3 antibody

Mouse splenocytes stained with Hamster anti Mouse CD3 (MBS214442)
Hamster Anti-CD3 Monoclonal Antibody, Testing Data – Cat# MBS216295

Other Notes

Small volumes of anti-CD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD3 purchase

MBS216295 is an antibody targeting CD3. Also known as HAMSTER ANTI MOUSE CD3. Host: Hamster.

Additional Details

NCBI/Uniprot data below describe general gene information for CD3. It may not necessarily be applicable to this product.

NCBI GI #
6680886
NCBI GeneID
12501
NCBI Accession #
NP_031674.1
UniProt Primary Accession #
P22646
UniProt Related Accession #
P22646
GenBank Nucleotide #
NM_007648.4
NCBI Official Full Name
T-cell surface glycoprotein CD3 epsilon chain
NCBI Official Synonym Full Names
CD3 antigen, epsilon polypeptide
NCBI Official Symbol
Cd3e
NCBI Official Synonym Symbols
CD3; T3e; AI504783; CD3epsilon
UniProt Protein Name
T-cell surface glycoprotein CD3 epsilon chain
UniProt Synonym Protein Names
T-cell surface antigen T3/Leu-4 epsilon chain; CD_antigen: CD3e
Protein Family
CD302 antigen
UniProt Gene Name
Cd3e
UniProt Entry Name
CD3E_MOUSE
NCBI Protein Information
T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain

UniProt Comments for CD3

CD3E: a T cell surface glycoprotein that is a component of the T cell antigen receptor. The recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Contains 1 immunoglobulin-like domain and 1 ITAM domain.

Protein type: Receptor, misc.; Membrane protein, integral

Cellular Component: T cell receptor complex; membrane; integral to membrane; plasma membrane; immunological synapse; alpha-beta T cell receptor complex; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; signal transducer activity; transmembrane receptor activity; protein heterodimerization activity; SH3 domain binding

Biological Process: positive regulation of interleukin-2 biosynthetic process; lymphocyte activation; positive regulation of calcium-mediated signaling; negative thymic T cell selection; T cell receptor signaling pathway; positive regulation of interleukin-4 production; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of interferon-gamma production; cell surface receptor linked signal transduction; negative regulation of smoothened signaling pathway; T cell costimulation; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell proliferation; positive regulation of T cell activation; positive regulation of T cell anergy; response to nutrient
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Research Articles on CD3

1. these results suggest that CD3zeta can be degraded by two pathways: SLAP/c-Cbl, which targets internalized cell surface CD3zeta dependent on TCR signaling, and LAPTM5, which targets intracellular CD3zeta independent of TCR signaling.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD3 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Downstream TCR Signaling Pathway antibodies  Downstream TCR Signaling Pathway Diagram
 Generation Of Second Messenger Molecules Pathway antibodies  Generation Of Second Messenger Molecules Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram

Diseases associated with anti-CD3 antibody

 Disease Name  Pubmed Publications
 Lymphoma Antibodies  >26 publications with CD3 and Lymphoma
 Inflammation Antibodies  >20 publications with CD3 and Inflammation
 Disease Models, Animal Antibodies  >16 publications with CD3 and Disease Models, Animal
 Immunologic Deficiency Syndromes Antibodies  >14 publications with CD3 and Immunologic Deficiency Syndromes
 Necrosis Antibodies  >13 publications with CD3 and Necrosis
 Neoplasms, Experimental Antibodies  >7 publications with CD3 and Neoplasms, Experimental
 Severe Combined Immunodeficiency Antibodies  >5 publications with CD3 and Severe Combined Immunodeficiency
 Carcinoma Antibodies  >5 publications with CD3 and Carcinoma
 Lung Diseases Antibodies  >5 publications with CD3 and Lung Diseases
 Arthritis, Rheumatoid Antibodies  >5 publications with CD3 and Arthritis, Rheumatoid

Organs/Tissues associated with anti-CD3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >190 publications with CD3 and Blood
 Thymus Antibodies  >46 publications with CD3 and Thymus
 Spleen Antibodies  >35 publications with CD3 and Spleen
 Bone Marrow Antibodies  >26 publications with CD3 and Bone Marrow
 Mammary Gland Antibodies  >1 publications with CD3 and Mammary Gland
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