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CD52 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS216359

MBS216359 reacts with the human CD52 antigen, also known as CAMPATH-1. The CD52 antigen is a remarkably small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on… More details

$460 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD52, Monoclonal Antibody
Full Product Name
RAT ANTI HUMAN CD52
Product Gene Name
Clonality
Monoclonal
Isotype
IgM
Clone Number
YTH66.9HL
Host
Rat
Form/Format
Purified
Ig fraction - liquid
Concentration
IgM concentration 2 mg/ml (lot specific)
Specificity
This item reacts with the human CD52 antigen, also known as CAMPATH-1. The CD52 antigen is a remarkably small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29kDa. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable. Humanised versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies.
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
6,614 Da
OMIM
114280
3D Structure
ModBase 3D Structure for P31358
Perservative Stabilisers
0.09% Sodium Azide
Preparation||Ig fraction prepared by ammonium sulphate precipitation from tissue culture supernatant.
Immunogen
Human T lymphocytes
Fusion Partners||Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag.1.2.3 myeloma cell line.
Buffer Solution||Phosphate buffered saline
Target Species
Human
Applications Tested/Suitable for anti-CD52 antibody
Immunohistology Frozen, Cytotoxic Assays (CT), Flow cytometry (FC/FACS), Western Blot (WB)
Application Notes for anti-CD52 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology - Frozen: Maximum Dilution: 1/20
Flow Cytometry: Maximum Dilution: 1/20

Other Notes

Small volumes of anti-CD52 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD52 purchase

MBS216359 is an antibody targeting CD52. Also known as RAT ANTI HUMAN CD52. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD52. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
CAMPATH-1 antigen
NCBI Official Synonym Full Names
CD52 molecule
NCBI Official Symbol
CD52
NCBI Official Synonym Symbols
CDW52
UniProt Protein Name
CAMPATH-1 antigen
UniProt Synonym Protein Names
CDw52; Cambridge pathology 1 antigen; Epididymal secretory protein E5; Human epididymis-specific protein 5; He5; CD_antigen: CD52
Protein Family
UniProt Gene Name
CD52
UniProt Synonym Gene Names
CDW52; HE5; He5
UniProt Entry Name
CD52_HUMAN
NCBI Protein Information
CAMPATH-1 antigen; CD52 antigen (CAMPATH-1 antigen); CDW52 antigen (CAMPATH-1 antigen); HEL-S-171mP; cambridge pathology 1 antigen; epididymal secretory protein E5; epididymis secretory sperm binding protein Li 171mP; he5; human epididymis-specific protein 5

UniProt Comments for CD52

CD52: May play a role in carrying and orienting carbohydrate, as well as having a more specific role.

Protein type: Membrane protein, GPI anchor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: membrane; integral to plasma membrane

Biological Process: respiratory burst; elevation of cytosolic calcium ion concentration
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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