Rat Anti-F4/80 Monoclonal Antibody – Cat# MBS216416

F4/80 Antibody – Rat Monoclonal

Name: F4/80 Antibody; Monoclonal; Rat; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS216416
Price: 255 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS216416

MBS216416 is an antibody targeting F4/80. Also known as RAT ANTI MOUSE F4/80:FITC. Reactive in Mouse. Host: Rat. More details

$255 / 0.025 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

F4/80, Monoclonal Antibody

Full Product Name

RAT ANTI MOUSE F4/80:FITC

Product Gene Name

anti-F4/80 antibody

Product Synonym Names

Gpf480

Clonality

Monoclonal

Isotype

IgG2b

Clone Number

A3-1

Host

Rat

Species Reactivity

Mouse

Form/Format

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Concentration

IgG concentration 0.1 mg/ml (lot specific)

Specificity

Rat anti mouse F4/80 antibody, clone Cl:A3-1 recognises the murine F4/80 antigen, a ~160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).
Expression of F4/80 is heterogeneous and is modulated during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans cells, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen (Hume, et al. 1984). F4/80 antigen is also expressed on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node (Gordon, et al. 1994). The ligands and biological functions of the F4/80 antigen have not been fully determined but a role for F4/80 in the generation of efferent CD8+ve regulatory T cells is proposed (Lin, et al. 2005)
Rat anti mouse F4/80 antibody, clone Cl:A3-1 modulates cytokine levels released in response to Listeria monocytogenes (Warschkau & Kiderlen, 1999).
A Human anti-idiotypic CI:A31 antibody, clone 17867 (HCA154 ) which binds to and blocks activity of Rat anti mouse F4/80 antibody, clone Cl:A3-1 is also available for use as a control in experiments utilizing clone A3-1.

Preparation and Storage

Store at +4°C or at -20°C if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

3D Structure

ModBase 3D Structure for Q61549

Target Species

Mouse

Max Ex/Em

Fluorophore: FITC
Excitation Max (nm): 490
Emission Max (nm): 525

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

Buffer Solution

Phosphate buffered saline

Preservative Stabilizers

0.09% Sodium Azide
1% Bovine Serum Albumin

Immunogen

Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.

Fusion Partners

Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.

Applications Tested/Suitable for anti-F4/80 antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF)

Application Notes for anti-F4/80 antibody

Flow Cytometry (FC/FACS) - Suggested Dilution: Neat / Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. The suggested working dilution is given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Other Notes

Small volumes of anti-F4/80 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for F4/80. It may not necessarily be applicable to this product.

NCBI GI #

33859546

NCBI GeneID

13733

NCBI Accession #

NP_034260.1

UniProt Primary Accession #

Q61549

UniProt Related Accession #

Q61549

GenBank Nucleotide #

NM_010130.4

NCBI Official Full Name

EGF-like module-containing mucin-like hormone receptor-like 1

NCBI Official Synonym Full Names

EGF-like module containing, mucin-like, hormone receptor-like sequence 1

NCBI Official Symbol

Emr1

NCBI Official Synonym Symbols

Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7

UniProt Protein Name

EGF-like module-containing mucin-like hormone receptor-like 1

UniProt Synonym Protein Names

Cell surface glycoprotein F4/80; EGF-like module receptor 1; EMR1 hormone receptor

UniProt Gene Name

Emr1

UniProt Synonym Gene Names

Gpf480

UniProt Entry Name

EMR1_MOUSE

NCBI Protein Information

EGF-like module-containing mucin-like hormone receptor-like 1; EGF-like module receptor 1; EMR1 hormone receptor; cell surface glycoprotein F4/80; lymphocyte antigen 71

UniProt Comments for F4/80

EMR1: Could be involved in cell-cell interactions. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 2; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass

Cellular Component: membrane; integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: G-protein coupled receptor activity; signal transducer activity; transmembrane receptor activity; calcium ion binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; signal transduction

Research Articles on F4/80

1. EMR1 expression is restricted to mature blood and tissue eosinophils. Targeting of eosinophils with afucosylated anti-EMR1 antibody shows promise as a treatment for eosinophilic disorders.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-F4/80 antibody

Products by Pathway	Pathway Diagram
GPCRs, Class B Secretin-like Pathway antibodies	GPCRs, Class B Secretin-like Pathway Diagram
Non-odorant GPCRs Pathway antibodies	Non-odorant GPCRs Pathway Diagram

Diseases associated with anti-F4/80 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>31 publications with F4/80 and Inflammation
Insulin Resistance Antibodies	>15 publications with F4/80 and Insulin Resistance
Necrosis Antibodies	>8 publications with F4/80 and Necrosis
Liver Diseases Antibodies	>7 publications with F4/80 and Liver Diseases
Fibrosis Antibodies	>6 publications with F4/80 and Fibrosis
Fatty Liver Antibodies	>6 publications with F4/80 and Fatty Liver
Cardiovascular Diseases Antibodies	>4 publications with F4/80 and Cardiovascular Diseases
Atherosclerosis Antibodies	>3 publications with F4/80 and Atherosclerosis
Weight Gain Antibodies	>3 publications with F4/80 and Weight Gain
Weight Loss Antibodies	>2 publications with F4/80 and Weight Loss

Organs/Tissues associated with anti-F4/80 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>31 publications with F4/80 and Blood
Connective Tissue Antibodies	>15 publications with F4/80 and Connective Tissue
Liver Antibodies	>12 publications with F4/80 and Liver
Muscle Antibodies	>6 publications with F4/80 and Muscle
Spleen Antibodies	>4 publications with F4/80 and Spleen
Bone Antibodies	>3 publications with F4/80 and Bone
Bone Marrow Antibodies	>3 publications with F4/80 and Bone Marrow
Embryonic Tissue Antibodies	>2 publications with F4/80 and Embryonic Tissue
Kidney Antibodies	>2 publications with F4/80 and Kidney
Lung Antibodies	>2 publications with F4/80 and Lung