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CD54 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS216459

Mouse anti Human CD54 antibody, clone 84H10 recognizes the D1 domain of ICAM-1. It reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. This molecule is inducible to high levels by mitogenic… More details

$420 / 0.025 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CD54, Monoclonal Antibody
Full Product Name
MOUSE ANTI HUMAN CD54
Product Gene Name
anti-CD54 antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
84H10
Host
Mouse
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
57,825 Da
OMIM
147840
3D Structure
ModBase 3D Structure for P05362
Perservative Stabilisers
0.09% Sodium Azide
Preparation||Purified IgG prepared by affinity chromatography on Protein A from ascites
Immunogen
K562 cell line
Fusion Partners||Spleen cells from immunised BALB/c mice were fused with cells of the MOPC 315 mouse myeloma cell line.
Buffer Solution||Phosphate buffered saline
Target Species
Human
Applications Tested/Suitable for anti-CD54 antibody
Immunohistology Frozen*, ELISA (EIA), Flow cytometry (FC/FACS), Functional Assays (FN)*, Immunoprecipitation (IP)
Application Notes for anti-CD54 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology - Frozen: Minimum Dilution: 1/20; Maximum Dilution: 1/50; Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10
Functional Assays: Application Note: Removal of sodium azide is recommended prior to use in functional assays.
Immunoprecipitation: Maximum Dilution: 5ug / 10e7 cells

Testing Data of anti-CD54 antibody

Published customer image: ICAM-1 activates PKCa but not PKCbetaII in HMVECLs. A) At 70% confluence, HMVECLs cells pretreated with 10 ng/ml TNFa to induce ICAM-1 expression. At 24 hrs, the cells were suspended and immunolabeled with anti-ICAM-1 antibodies and examined by flow cytometry for ICAM-1 expression. B -D) HMVECLs were pretreated with TNFa as in panel A. At 24 hrs, the endothelial cells were nonstimulated (NS) or stimulated with a confluent monolayer of anti-ICAM-1-coated beads or the control anti-PECAM-1-coated beads for 20 minutes in B and D or for the times indicated in C. The cells were lysed and the activation of PKCbetaII and PKCa was examined by western blot with B) anti-phospho-PKCbetaIIThr641 or anti-PKCbetaII or C -D) anti-phospho- PKCaThr638 or anti-PKCa. Shown are representative blots. Shown are the mean +/- SEM of 3 experiments. NT, nontreated. *, p
Mouse Anti-CD54 Monoclonal Antibody – Cat# MBS216459

Other Notes

Small volumes of anti-CD54 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD54 purchase

MBS216459 is an antibody targeting CD54. Also known as MOUSE ANTI HUMAN CD54. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD54. It may not necessarily be applicable to this product.

NCBI GI #
167466198
NCBI GeneID
3383
NCBI Accession #
NP_000192.2
UniProt Primary Accession #
P05362
UniProt Secondary Accession #
Q5NKV7; Q96B50; B2R6M3
UniProt Related Accession #
P05362
GenBank Nucleotide #
NM_000201.2
NCBI Official Full Name
intercellular adhesion molecule 1
NCBI Official Synonym Full Names
intercellular adhesion molecule 1
NCBI Official Symbol
ICAM1
NCBI Official Synonym Symbols
BB2; CD54; P3.58
UniProt Protein Name
Intercellular adhesion molecule 1
UniProt Synonym Protein Names
Major group rhinovirus receptor; CD_antigen: CD54
Protein Family
Intercellular adhesion molecule
UniProt Gene Name
ICAM1
UniProt Synonym Gene Names
ICAM-1
UniProt Entry Name
ICAM1_HUMAN
NCBI Protein Information
intercellular adhesion molecule 1; ICAM-1; cell surface glycoprotein P3.58; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; major group rhinovirus receptor

NCBI Summary for CD54

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]

UniProt Comments for CD54

ICAM1: a type I membrane protein of the immunoglobulin superfamily. Is a ligand for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2) and a Rhinovirus receptor. Typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. Its expression is activated by p53 in an NF-kappaB-independent manner. Induced by TNFalpha in a process that involves IKKbeta.

Protein type: Membrane protein, integral; Cell adhesion; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: extracellular space; focal adhesion; cell surface; membrane; integral to plasma membrane; plasma membrane; immunological synapse; external side of plasma membrane

Molecular Function: integrin binding; viral receptor activity; protein binding; transmembrane receptor activity; receptor activity

Biological Process: extracellular matrix organization and biogenesis; entry of virus into host cell; positive regulation of nitric oxide biosynthetic process; T cell antigen processing and presentation; response to organic cyclic substance; activation of NF-kappaB transcription factor; positive regulation of cellular extravasation; regulation of cell shape; cellular response to nutrient levels; leukocyte adhesion; sensory perception of sound; ovarian follicle development; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; membrane to membrane docking; response to sulfur dioxide; cell adhesion; regulation of leukocyte mediated cytotoxicity; acute inflammatory response to antigenic stimulus; response to drug; regulation of cell adhesion; virion attachment, binding of host cell surface receptor; regulation of immune response; negative regulation of calcium ion transport; cytokine and chemokine mediated signaling pathway; response to amphetamine; cell aging; response to amino acid stimulus; heterophilic cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; response to copper ion; positive regulation of actin filament polymerization; positive regulation of vasoconstriction; cell adhesion mediated by integrin; adhesion to host; response to ionizing radiation; leukocyte migration

Disease: Malaria, Susceptibility To
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Research Articles on CD54

1. ICAM1 is likely to be involved in the development of childhood asthma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD54 antibody

 Reference Product  PubMed Publications
 ITGB2 antibody  >880 publications with CD54 and ITGB2
 ITGAL antibody  >474 publications with CD54 and ITGAL
 ITGAM antibody  >440 publications with CD54 and ITGAM
 CD44 antibody  >371 publications with CD54 and CD44
 IL6 antibody  >153 publications with CD54 and IL6
 ITGAX antibody  >139 publications with CD54 and ITGAX
 SELE antibody  >87 publications with CD54 and SELE
 HLA-B antibody  >29 publications with CD54 and HLA-B
 DIF antibody  >3 publications with CD54 and DIF

Pathways associated with anti-CD54 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram

Diseases associated with anti-CD54 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >5639 publications with CD54 and Inflammation
 Necrosis Antibodies  >4797 publications with CD54 and Necrosis
 Cardiovascular Diseases Antibodies  >3657 publications with CD54 and Cardiovascular Diseases
 Atherosclerosis Antibodies  >1470 publications with CD54 and Atherosclerosis
 Reperfusion Injury Antibodies  >884 publications with CD54 and Reperfusion Injury
 Myocardial Ischemia Antibodies  >797 publications with CD54 and Myocardial Ischemia
 Kidney Diseases Antibodies  >760 publications with CD54 and Kidney Diseases
 Hypertension Antibodies  >527 publications with CD54 and Hypertension
 Asthma Antibodies  >502 publications with CD54 and Asthma
 Stroke Antibodies  >388 publications with CD54 and Stroke

Organs/Tissues associated with anti-CD54 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >11797 publications with CD54 and Blood
 Vascular Antibodies  >8826 publications with CD54 and Vascular
 Lung Antibodies  >1776 publications with CD54 and Lung
 Heart Antibodies  >1288 publications with CD54 and Heart
 Skin Antibodies  >1198 publications with CD54 and Skin
 Liver Antibodies  >1180 publications with CD54 and Liver
 Kidney Antibodies  >1177 publications with CD54 and Kidney
 Brain Antibodies  >1177 publications with CD54 and Brain
 Muscle Antibodies  >1049 publications with CD54 and Muscle
 Embryonic Tissue Antibodies  >774 publications with CD54 and Embryonic Tissue
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