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CD4 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS216479

MBS216479 is an antibody targeting CD4. Also known as MOUSE ANTI RAT CD4 (DOMAIN 1):FITC. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CD4, Monoclonal Antibody
Full Product Name
MOUSE ANTI RAT CD4 (DOMAIN 1):FITC
Product Gene Name
anti-CD4 antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
W3/25
Host
Mouse
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
51,438 Da
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Rat thymocyte membrane glycoproteins
Fusion Partners||Spleen cells from immunised BALB/c mouse were fused with cells of the mouse NS-1 myeloma cell line.
Buffer Solution||Phosphate buffered saline
Target Species
Rat
Applications Tested/Suitable for anti-CD4 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD4 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10

Testing Data of anti-CD4 antibody

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. High power
Mouse Anti-CD4 Monoclonal Antibody – Cat# MBS216479

Testing Data of anti-CD4 antibody

Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. Low power
Mouse Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS216479

Testing Data of anti-CD4 antibody

Published customer image: Dynamics of T-regulatory cells. D -F) Photographs of double immunolabelled sections showing a representative CD4+Foxp3+ cell (arrows) found around blood vessels (BV). Bar scale = 30 um.From: Almolda B, Costa M, Montoya M, Gonz¡lez B, Castellano B (2011) Increase in Th17 and T-reg Lymphocytes and Decrease of IL22 Correlate with the Recovery Phase of Acute EAE IN Rat. PLoS ONE 6(11): e27473.
Mouse Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS216479

Testing Data of anti-CD4 antibody

Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. Medium power
Mouse Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS216479

Testing Data of anti-CD4 antibody

Staining of stimulated rat spleen cells with Mouse anti Rat CD4 (MBS213091)
Mouse Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS216479

Testing Data of anti-CD4 antibody

Immunoperoxidase staining of rat lymph node cryosetion using Mouse anti Rat CD4 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 for detection. Low power
Mouse Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS216479

Testing Data of anti-CD4 antibody

Staining of rat splenocytes with Mouse anti Rat CD4 (MBS210505)
Mouse Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS216479

Testing Data of anti-CD4 antibody

Immunoperoxidase staining of rat lymph node cryosetion using Mouse anti Rat CD4 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 for detection. High power
Mouse Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS216479

Testing Data of anti-CD4 antibody

Staining of frozen rat spleen with Mouse anti Rat CD4
Mouse Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS216479

Testing Data of anti-CD4 antibody

Published customer image: Dynamics of Th17 cells. D -F) Photographs of double immunolabelled sections showing a representative CD4+ROR?+ cell (arrow) observed around blood vessels (BV). Bar scale = 30 um.Almolda B, Costa M, Montoya M, Gonz¡lez B, Castellano B (2011) Increase in Th17 and T-reg Lymphocytes and Decrease of IL22 Correlate with the Recovery Phase of Acute EAE IN Rat. PLoS ONE 6(11): e27473.
Mouse Anti-CD4 Monoclonal Antibody, Testing Data – Cat# MBS216479

Other Notes

Small volumes of anti-CD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD4. It may not necessarily be applicable to this product.

NCBI GI #
6978631
NCBI GeneID
24932
NCBI Accession #
NP_036837.1
UniProt Related Accession #
P05540
GenBank Nucleotide #
NM_012705.1
NCBI Official Full Name
T-cell surface glycoprotein CD4
NCBI Official Synonym Full Names
Cd4 molecule
NCBI Official Symbol
Cd4
NCBI Official Synonym Symbols
p55; W3/25
UniProt Protein Name
T-cell surface glycoprotein CD4
UniProt Synonym Protein Names
T-cell surface antigen T4/Leu-3; W3/25 antigen; CD_antigen: CD4
Protein Family
T-cell surface glycoprotein
UniProt Gene Name
Cd4
UniProt Entry Name
CD4_RAT
NCBI Protein Information
T-cell surface glycoprotein CD4; CD4 antigen; T-cell surface antigen T4/Leu-3; W3/25 antigen

NCBI Summary for CD4

MHC class II binding protein that may be a candidate gene for collagen-induced arthritis; human CD4 is a receptor required for HIV infection [RGD, Feb 2006]

UniProt Comments for CD4

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.

Research Articles on CD4

1. Data show several homologies across human, rat and mouse in the kinetic of loss of several LAgs such as CD5, CD4 and CD28.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD4 antibody

 Reference Product  PubMed Publications
 Foxp3 antibody  >7195 publications with CD4 and Foxp3
 Ptprc antibody  >2890 publications with CD4 and Ptprc
 Ccr7 antibody  >840 publications with CD4 and Ccr7
 Lck antibody  >797 publications with CD4 and Lck
 Zap70 antibody  >213 publications with CD4 and Zap70
 Cd8a antibody  >83 publications with CD4 and Cd8a
 Cd247 antibody  >6 publications with CD4 and Cd247
 Lcp2 antibody  >1 publications with CD4 and Lcp2
 RT1-Da antibody  >1 publications with CD4 and RT1-Da

Pathways associated with anti-CD4 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Alpha-defensins Pathway antibodies  Alpha-defensins Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Cytokines And Inflammatory Response (BioCarta) Pathway antibodies  Cytokines And Inflammatory Response (BioCarta) Pathway Diagram
 Defensins Pathway antibodies  Defensins Pathway Diagram
 Downstream TCR Signaling Pathway antibodies  Downstream TCR Signaling Pathway Diagram

Diseases associated with anti-CD4 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >12218 publications with CD4 and Inflammation
 Disease Models, Animal Antibodies  >9067 publications with CD4 and Disease Models, Animal
 Necrosis Antibodies  >7648 publications with CD4 and Necrosis
 Lung Diseases Antibodies  >6768 publications with CD4 and Lung Diseases
 Liver Diseases Antibodies  >4684 publications with CD4 and Liver Diseases
 AIDS-Related Opportunistic Infections Antibodies  >4364 publications with CD4 and AIDS-Related Opportunistic Infections
 Carcinoma Antibodies  >3428 publications with CD4 and Carcinoma
 Cardiovascular Diseases Antibodies  >3187 publications with CD4 and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >2980 publications with CD4 and Neoplasms, Experimental
 Vascular Diseases Antibodies  >2496 publications with CD4 and Vascular Diseases

Organs/Tissues associated with anti-CD4 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >96981 publications with CD4 and Blood
 Spleen Antibodies  >9465 publications with CD4 and Spleen
 Thymus Antibodies  >7207 publications with CD4 and Thymus
 Heart Antibodies  >2602 publications with CD4 and Heart
 Muscle Antibodies  >1718 publications with CD4 and Muscle
 Prostate Antibodies  >361 publications with CD4 and Prostate
 Adipose Tissue Antibodies  >295 publications with CD4 and Adipose Tissue
 Pituitary Gland Antibodies  >71 publications with CD4 and Pituitary Gland
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