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CD169 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS216689

Rat anti Mouse CD169, clone MOMA-1 recognizes murine CD169, also known as sialoadhesin or Siglec-1. CD169 is a lectin-like receptor expressed by certain populations of macrophages including marginal zone metallophils of the spleen, subcapsular macrophages of… More details

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Product Overview

Product Name
CD169, Monoclonal Antibody
Full Product Name
RAT ANTI MOUSE CD169
Product Gene Name
anti-CD169 antibody
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
MOMA-1
Host
Rat
Form/Format
Tissue Culture Supernatant - liquid
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
172,769 Da
3D Structure
ModBase 3D Structure for Q62230
Perservative Stabilisers
0.09% Sodium Azide
Preparation||Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Target Species
Mouse
Applications Tested/Suitable for anti-CD169 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-CD169 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Immunohistology - Frozen: Maximum Dilution: 1/10

Testing Data of anti-CD169 antibody

Published customer image: Intrafollicular location of MZB cells in B6.TC spleens. A. Representative spleen sections from 3 mo old B6.TC (left) and B6 (right) mice stained with Moma-1-FITC, CD1d-PE and B220-PB. The B220+ CD1d+ MZB cells show as bright pink while the other B cells show as blue. The ring of Moma-1+ metallophillic macrophages delineates the MZ inner edge. B. Percentage of CD1d+ B220+ B cells relative to total B220+ B cells outside (MZ) and inside (FO) the Moma-1+ ring in 3 mo and 10 mo old B6 and B6.TC mice. The data show means and standard errors of the mean (SEM) calculated of 4 MZ and FO areas for each mouse. ***: p < 0.001 for t tests. C. Representative spleen sections from 10 mo old B6.TC (left) and B6 (right) mice stained with CD4-FITC, CD1d-PE and B220-PB. Boxed areas show multiple contacts between green B6.TC CD4 T cells and pink MZB cells, but not in the B6 spleen. Original magnification: 200X.From: Zhou et al. BMC Immunology 2011 12:7.
Rat Anti-CD169 Monoclonal Antibody – Cat# MBS216689

Testing Data of anti-CD169 antibody

Immunofluorescence staining of mouse spleen with Rat anti Mouse CD169 (MBS216690)
Rat Anti-CD169 Monoclonal Antibody, Testing Data – Cat# MBS216689

Testing Data of anti-CD169 antibody

Published customer image: Intrafollicular location of 56R and AM14 HC Tg MZB cells. Representative spleen sections of B6.Sle2.56R and B6.56R (A, 100X) and B6.TC.AM14.IgHa/b and B6.AM14.IgHa/b (B, 200X) mice stained with Moma-1-FITC, CD1d-PE, and IgMa-biotin-SA-PB. C. Quantitation in pixels corresponding to the stain combination specific for each cell type of CD1d+ IgMa cells in the MZ (pink) and corresponding FO (blue) B cell areas in B6.Sle2.56R, B6.56R, B6.TC.AM14.IgHa/b and B6.AM14.IgHa/b mice. Paired MZ and FO values within a strain were compared with the Wilcoxon signed ranked test, and the B6.TC.AM14.IgHa/b and B6.AM14.IgHa/b MZ values were compared with the Mann-Whitney test. CD21hi CD23hi representing the T2 and MZB precursor cells expressed as the percentage of transgenic IgMa cells (D), and percentage of CD21hi CD23lo MZ B cells expressed as the percentage of transgenic IgMa cells (E) and their absolute numbers (F) in B6.Sle2.56R and B6.56R and B6.TC.AM14.IgHa/b and B6.AM14.IgHa/b mice. Graphs show means and SEMs, and the statistical significance of Student t tests. *: p < 0.05, **: p < 0.01, ***: p < 0.001.From: Zhou et al. BMC Immunology 2011 12:7.
Rat Anti-CD169 Monoclonal Antibody, Testing Data – Cat# MBS216689

Other Notes

Small volumes of anti-CD169 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD169 purchase

MBS216689 is an antibody targeting CD169. Also known as RAT ANTI MOUSE CD169. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD169. It may not necessarily be applicable to this product.

NCBI GI #
226958332
NCBI GeneID
20612
NCBI Accession #
NP_035556.3
UniProt Primary Accession #
Q62230
UniProt Secondary Accession #
O55216; Q62228; Q62229; D3YVZ3; D3YVZ4
UniProt Related Accession #
Q62230
GenBank Nucleotide #
NM_011426.3
NCBI Official Full Name
sialoadhesin
NCBI Official Synonym Full Names
sialic acid binding Ig-like lectin 1, sialoadhesin
NCBI Official Symbol
Siglec1
NCBI Official Synonym Symbols
Sn; Cd169; Siglec-1
UniProt Protein Name
Sialoadhesin
UniProt Synonym Protein Names
Sheep erythrocyte receptor; SER; Sialic acid-binding Ig-like lectin 1; Siglec-1; CD_antigen: CD169
UniProt Gene Name
Siglec1
UniProt Synonym Gene Names
Sa; Sn; SER; Siglec-1
UniProt Entry Name
SN_MOUSE
NCBI Protein Information
sialoadhesin; SER; sheep erythrocyte receptor; sialic acid-binding Ig-like lectin 1

UniProt Comments for CD169

SIGLEC1: Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid. Binds to SPN/CD43 on T-cells. May play a role in hemopoiesis. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: membrane; integral to membrane; plasma membrane; extracellular region

Molecular Function: protein binding; carbohydrate binding

Biological Process: endocytosis; cell adhesion
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Research Articles on CD169

1. Sn deficiency does not have a significant effect on the severity and progression of lupus nephritis in the NZBWF1 mouse model.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD169 antibody

 Products by Pathway  Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram

Diseases associated with anti-CD169 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >19 publications with CD169 and Inflammation
 Nervous System Diseases Antibodies  >9 publications with CD169 and Nervous System Diseases
 Skin Diseases Antibodies  >7 publications with CD169 and Skin Diseases
 Necrosis Antibodies  >7 publications with CD169 and Necrosis
 Cardiovascular Diseases Antibodies  >6 publications with CD169 and Cardiovascular Diseases
 Carcinoma Antibodies  >5 publications with CD169 and Carcinoma
 Atherosclerosis Antibodies  >4 publications with CD169 and Atherosclerosis
 Lung Diseases Antibodies  >4 publications with CD169 and Lung Diseases
 Bone Marrow Diseases Antibodies  >4 publications with CD169 and Bone Marrow Diseases
 Respiratory Tract Diseases Antibodies  >4 publications with CD169 and Respiratory Tract Diseases

Organs/Tissues associated with anti-CD169 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >25 publications with CD169 and Bone
 Lymph Node Antibodies  >20 publications with CD169 and Lymph Node
 Lung Antibodies  >4 publications with CD169 and Lung
 Brain Antibodies  >4 publications with CD169 and Brain
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